In Situ Hybridization Protocols, Nielsen Boye Schnack, Jones Julia

Автор: Nuovo Gerard J.
Название: In Situ Molecular Pathology And Co-Expression Analyses
ISBN: 0128206535 ISBN-13(EAN): 9780128206539
Издательство: Elsevier Science
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Цена: 15210.00 р. 21728.00 -30%
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Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-Expression Analyses, Second Edition continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other cook-book type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry.

Автор: J.G. Hawkes; Nigel Maxted; B.V. Ford-Lloyd
Название: The Ex Situ Conservation of Plant Genetic Resources
ISBN: 0792364422 ISBN-13(EAN): 9780792364429
Издательство: Springer
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Описание: Article 8 of the Convention clearly states the need to develop more effective and efficient guidelines to conserve biological diversity, while Article 9, along with the FAO International Undertaking on Plant Genetic Resources, promotes the adoption of a complementary approach to conservation that incorporates both ex situ and in situ techniques.

Автор: Liehr
Название: Fluorescence In Situ Hybridization (FISH)
ISBN: 3662529572 ISBN-13(EAN): 9783662529577
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.

Автор: Giselbert Hauptmann
Название: In Situ Hybridization Methods
ISBN: 1493923021 ISBN-13(EAN): 9781493923021
Издательство: Springer
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Цена: 17468.00 р.
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Описание: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications.