Описание: This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.
Автор: Bhupendra Pushkar Название: Protein Purification ISBN: 1774077027 ISBN-13(EAN): 9781774077023 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 22730.00 р. Наличие на складе: Нет в наличии.
Описание: Describes the concepts underlying the process of protein purification. The strategies and equipment needed in protein purification is described. Advanced techniques like Polyacrylamide Gel Electrophoresis and Mass spectroscopy are also explained.
Автор: Argelia Lorence Название: Recombinant Gene Expression ISBN: 1493962213 ISBN-13(EAN): 9781493962211 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Recombinant gene expression research has brought new advances such as the birth of the `omics` technologies. Here, expert researchers detail many RGE methods now used, including those for bacteria, fungi, plants and plant cells, and animals and animal cells.
Автор: Hacker Название: Recombinant Protein Expression in Mammalian Cells ISBN: 1493987291 ISBN-13(EAN): 9781493987290 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Автор: David L. Hacker Название: Recombinant Protein Expression in Mammalian Cells ISBN: 1493993771 ISBN-13(EAN): 9781493993772 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Поставка под заказ.
Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
Автор: Jacqueline MacDonald; Igor Kolotilin; Rima Menassa Название: Recombinant Proteins from Plants ISBN: 1493932888 ISBN-13(EAN): 9781493932887 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This volume provides up-to-date scientific achievements from the world's top researchers. RecombinantProteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RecombinantProteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.
Автор: Robert K. Scopes Название: Protein Purification ISBN: 0387940723 ISBN-13(EAN): 9780387940724 Издательство: Springer Рейтинг: Цена: 15672.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: New textbooks at all levels of chemistry appear with great regularity. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc- tive introductions to their fields.
Автор: Christopher Schirwitz Название: Purification of Peptides in High-Complexity Arrays ISBN: 3319008064 ISBN-13(EAN): 9783319008066 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book outlines an improvement of the quality of in situ synthesized high-complexity peptide micro arrays. It details the development of a new method allowing for fast one-step purification of entire arrays without loss of resolution or spatial information.
Автор: Richard R Burgess Название: Guide to Protein Purification,436 ISBN: 0123749786 ISBN-13(EAN): 9780123749789 Издательство: Elsevier Science Рейтинг: Цена: 12127.00 р. Наличие на складе: Поставка под заказ.
Описание: Examines some of the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology. This book relates how these traditional methods connect to the explosive advancements in the field. It includes chapters on both common and less-common relevant techniques.
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