Steger Gerhard Dnazymes 9781071620465

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Автор: Steger Gerhard
Название: Dnazymes
ISBN: 9781071620465
Издательство: Springer
Классификация:

Генетика (немедицинская)

Энзимология

ISBN-10: 1071620460
Обложка/Формат: Hardcover
Страницы: 330
Вес: 0.79 кг.
Дата издания: 01.03.2022
Серия: Methods in molecular biology
Язык: English
Издание: 1st ed. 2022
Иллюстрации: 77 illustrations, color; 18 illustrations, black and white; xvi, 312 p. 95 illus., 77 illus. in color.
Размер: 25.40 x 17.78 x 1.91 cm
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Acknowledgement...Preface...Table of Contents...Contributing Authors...
Part I SELEX1. A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzymeWenjing Li, Yanwei Cao, and Renjun Pei
2. Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzymeLuyan Yang, Yanwei Cao, and Renjun Pei
3. PCR Methods for the Generation of Catalytic ssDNADeni Szokoli, Kristian K. Le Vay, and Hannes Mutschler
Part II Design - Kinetics/Performance/Turnover
4. Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzymeGerhard Steger and Julian Victor
5. Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymesHannah Rosenbach and Gerhard Steger
6. Stability and Activity of the 10-23 DNAzyme under Molecular Crowding ConditionsNina Kirchgдssler, Hannah Rosenbach, and Ingrid Span
7. Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR SpectroscopyChristine Wuebben and Olav Schiemann
Part III Biophysical Characterization of DNAzymes
8. Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking MethodHannah Rosenbach and Ingrid Span
9. Crystallization and Structural Determination of 8-17 DNAzymeHehua Liu, Song Mao, Jia Sheng, and Jianhua Gan
10. Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and FunctionJan Borggrдfe and Manuel Etzkorn
11. Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model SystemChristoph G.W. Gertzen and Holger Gohlke
Part IV Further Biophysical Methods
12. Single-Molecule Kinetic Studies of Nucleic Acids by Fцrster Resonance Energy TransferMйlodie C.A.S. Hadzic, Roland K.O. Sigel, and Richard Bцrner
13. Chemical Dual End-Labeling of Large RibozymesEsra Ahunbay, Fabio D. Steffen, Susann Zelger-Paulus, and Roland K. O. Sigel
14. Spin Labeling of Long RNAs via Click Reaction and Enzymatic LigationMaria Francesca Vicino, Christine Wuebben, Mark Kerzhner, Michael Famulok, and Olav Schiemann
15. Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic AlphabetLisa Bornewasser and Stephanie Kath-Schorr
16. PELDOR Measurements on Nitroxide-Labelled OligonucleotidesTobias Hett and Olav Schiemann
Part V Use of DNAzymes
17. Sensing Metal Ions with Phosphorothioate-Modified DNAzymesPo-Jung Jimmy Huang and Juewen Liu
18. Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click ChemistryJoanna Kosman
19. Generation of RNA with 2, 3-cyclic Phosphates by Deoxyribozyme Cleavage in Frozen SolutionsKristian K. Le Vay and Hannes Mutschler
Subject Index List...

Дополнительное описание: A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme.- Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzyme.- PCR Methods for the Generation of Catalytic ssDNA.

Dnazymes, Steger Gerhard