Описание: Part I: Approaches to the Detection and Analysis of HPV Types
1. Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer
Zigui Chen, Luciana Bueno de Freitas, and Robert D. Burk
2. A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus
M. Isabel Micalessi, Gaelle A. Boulet, and J. Jean-Paul Bogers
Part II: Molecular Pathogenesis of CxCA
3. Replication of Human Papillomavirus in Culture
Eric J. Ryndock, Jennifer Biryukov, and Craig Meyers
4. HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocytes
Sarah A. Brendle and Neil D. Christensen
5. Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellular Proliferation and Induction of a DNA Damage Response
Michaлl Lehoux, Amйlie Fradet-Turcotte, and Jacques Archambault
6. Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle
Jason M. Bodily
7. Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes
Hsu-Kun Wang, Thomas R. Broker, and Louise T. Chow
8. A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Human Papillomavirus Types
David Gagnon and Jacques Archambault
9. Retroviral Expression of Human Cystatin Genes in HeLa Cells
Crystal M. Diep, Gagandeep Kaur, Daniel Keppler, and Athena W. Lin
Part III: Vaccinations and Immune Responses to HPV Infections
10. Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro
Andrew W. Woodham, Adam B. Raff, Diane M. Da Silva, and W. Martin Kast
Part IV: Identification of Novel Potential Drug Targets
11. Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells
Peralta-Zaragoza Oscar, De la O-Gуmez Faustino, Deas Jessica, Fernбndez-Tilapa Gloria, Fierros-Zбrate Geny del Socorro, Gуmez-Cerуn Claudia, Burguete-Garcнa Ana, Torres-Poveda Kirvis, Bermъdez-Morales Victor Hugo, Rodrнguez-Dorantes Mauricio, Pйrez-Plasencia Carlos, and Madrid-Marina Vicente
12. Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells
Wenyi Gu, Nigel McMillan, and Chengzhong Yu
13. Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays
Gina J. Ferris, Lauren B. Shunkwiler, and Charles A. Kunos
14. Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe
Colleen Rivard and Martina Bazzaro
Part V: Approaches to Detecting, Typing, and Staging of CxCA
15. Immunocytochemical Analysis of the Cervical Pap Smear
Terry K. Morgan and Michelle Berlin
16. Diagnosis of HPV-Negative, Gastric-Type Adenocarcinoma of the Endocervix
Edyta C. Pirog
17. Targeting of the HPV-16 E7 Protein by RNA Aptamers
Julia Dolores Toscano-Garibay, Marнa Luisa Benнtez-Hess, and Luis Marat Alvarez-Salas
18. The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer
Cara M. Martin, Katharine Astbury, Louise Kehoe, Jacqueline Barry O'Crowley, Sharon O'Toole, and John J. O'Leary
19. Fixation Methods for the Preservation of Morphology, RNAs, and Proteins in Paraffin-Embedded Human Cervical Cancer Cell Xenografts in Mice
Yoko Matsuda and Toshiyuki Ishiwata
Part VI: Genomic