Arabidopsis: A Practical Approach, 

Автор: Berrar Daniel P., Dubitzky Werner, Granzow Martin
Название: A Practical Approach to Microarray Data Analysis
ISBN: 1402072600 ISBN-13(EAN): 9781402072604
Издательство: Springer
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Цена: 4890.00 р. 6986.00 -30%
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Описание: A Practical Approach to Microarray Data Analysis is for all life scientists, statisticians, computer experts, technology developers, managers, and other professionals tasked with developing, deploying, and using microarray technology including the necessary computational infrastructure and analytical tools. The book addresses the requirement of scientists and researchers to gain a basic understanding of microarray analysis methodologies and tools. It is intended for students, teachers, researchers, and research managers who want to understand the state of the art and of the presented methodologies and the areas in which gaps in our knowledge demand further research and development. The book is designed to be used by the practicing professional tasked with the design and analysis of microarray experiments or as a text for a senior undergraduate- or graduate level course in analytical genetics, biology, bioinformatics, computational biology, statistics and data mining, or applied computer science. Key topics covered include: -Format of result from data analysis, analytical modeling/experimentation; -Validation of analytical results; -Data analysis/Modeling task; -Analysis/modeling tools; -Scientific questions, goals, and tasks; -Application; -Data analysis methods; -Criteria for assessing analysis methodologies, models, and tools.

Автор: Salinas Julio, Sanchez-Serrano Jose J.
Название: Arabidopsis Protocols
ISBN: 1588293955 ISBN-13(EAN): 9781588293954
Издательство: Springer
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Цена: 25853.00 р.
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Описание: This collection of readily reproducible Arabidopsis protocols has been updated to reflect recent advances in plant biology, the completion of the Arabidopsis genome sequence-essential for studying plant function, and the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics. The authors have included nearly all techniques developed in Arabidopsis, others recently adapted from the traditional work in crop species, and the most recent ones using Arabidopsis as a model system. Highlights include the most recent methods-transcriptomics, proteomics, and metabolomics-and their novel applications (phosphoproteomics, DNA microarray-based genotyping, high throughput metabolite profiling, and single-cell RNA).

Название: Epitope Mapping: A Practical Approach
ISBN: 0199636532 ISBN-13(EAN): 9780199636532
Издательство: Oxford Academ
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Цена: 22968.00 р.
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Описание: Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific eptiope. Investigation of carbohydrates can also be useful to eptitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.

Название: Arabidopsis: A Practical Approach
ISBN: 0199635641 ISBN-13(EAN): 9780199635641
Издательство: Oxford Academ
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Цена: 13464.00 р.
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Описание: Arabidopsis is known as the "Botanical Drosophila". With its small genome, low levels of repetitive DNA, small size and fast generation, it is an ideal molecular genetic tool for analyzing development in higher plants. This is an introduction to key techniques required for the use of Arabidopsis.