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RT-PCR Protocols, Connell Joe


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Цена: 8878р.
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Наличие: Отсутствует. Возможна поставка под заказ.

При оформлении заказа до: 25 окт 2019
Ориентировочная дата поставки: конец Ноября
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Автор: Connell Joe
Название:  RT-PCR Protocols   (Протоколы RT-PCR в реальном времени)
Издательство: Springer
Классификация:
Клеточная биология

ISBN: 0896038750
ISBN-13(EAN): 9780896038752
ISBN: 0-89603-875-0
ISBN-13(EAN): 978-0-89603-875-2
Обложка/Формат: Hardcover
Страницы: 380
Вес: 0.752 кг.
Дата издания: 2002
Серия: Methods in Molecular Biology
Язык: ENG
Размер: 23.42 x 15.49 x 2.67 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
Дополнительное описание: Формат: 229x152
Илюстрации: 100
Круг читателей: Biomedical researchers and molecular biologists working in university laboratories, hospital research and diagnostic laboratories, and in the biotechnology and pharmaceutical industries
Ключевые слова:
Язык: eng





PCR Cloning Protocols

Автор: Chen Bing-Yuan, Janes Harry W.
Название: PCR Cloning Protocols
ISBN: 0896039730 ISBN-13(EAN): 9780896039735
Издательство: Springer
Рейтинг:
Цена: 10280 р.
Наличие на складе: Поставка под заказ.

Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

PCR Cloning Protocols

Автор: Chen Bing-Yuan, Janes Harry W.
Название: PCR Cloning Protocols
ISBN: 0896039692 ISBN-13(EAN): 9780896039698
Издательство: Springer
Рейтинг:
Цена: 17297 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

PCR Sequencing Protocols

Автор: Rapley Ralph
Название: PCR Sequencing Protocols
ISBN: 0896033449 ISBN-13(EAN): 9780896033443
Издательство: Springer
Цена: 6821 р.
Наличие на складе: Поставка под заказ.

Описание: A superb, comprehensive collection of the most reliable protocols for PCR sequencing-all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.

PCR Protocols on CD (Institutional)

Автор: Walker John M.
Название: PCR Protocols on CD (Institutional)
ISBN: 1588294099 ISBN-13(EAN): 9781588294098
Издательство: Springer
Цена: 22347 р.
Наличие на складе: Поставка под заказ.

Описание: In the tradition of the acclaimed Methods in Molecular Biology series, this collection of more than 500 tried-and-tested PCR protocols will become the standard PCR reference set in all biological science laboratories. Comprehensive in scope, yet easily searchable, these protocols will enable PCR labs to have access to the widest array of PCR protocols available and thus be able to complete any kind of PCR experiment with ease. The protocols are presented in a step-by-step fashion and cover every aspect of PCR, making this an essential reference for all biological scientists.

PCR in Bioanalysis

Автор: Meltzer Stephen J
Название: PCR in Bioanalysis
ISBN: 0896034976 ISBN-13(EAN): 9780896034976
Издательство: Springer
Рейтинг:
Цена: 13557 р.
Наличие на складе: Поставка под заказ.

Описание: PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.

PCR Primer Design

Автор: Yuryev Anton
Название: PCR Primer Design
ISBN: 158829725X ISBN-13(EAN): 9781588297259
Издательство: Springer
Рейтинг:
Цена: 15894 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

PCR Mutation Detection Protocols

Автор: Theophilus Bimal D. M., Rapley Ralph
Название: PCR Mutation Detection Protocols
ISBN: 0896036170 ISBN-13(EAN): 9780896036178
Издательство: Springer
Цена: 6821 р.
Наличие на складе: Поставка под заказ.

Описание: Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Species Diagnostics Protocols / PCR and Other Nucleic Acid Methods

Автор: Clapp Justin P.
Название: Species Diagnostics Protocols / PCR and Other Nucleic Acid Methods
ISBN: 0896033236 ISBN-13(EAN): 9780896033238
Издательство: Springer
Рейтинг:
Цена: 17297 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification.

PCR Protocols on CD (Personal)

Автор: Walker John M.
Название: PCR Protocols on CD (Personal)
ISBN: 1588297136 ISBN-13(EAN): 9781588297136
Издательство: Springer
Рейтинг:
Цена: 11215 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: In the tradition of the acclaimed Methods in Molecular Biology series, this collection of more than 500 tried-and-tested PCR protocols will become the standard PCR reference set in all biological science laboratories. Comprehensive in scope, yet easily searchable, these protocols will enable PCR labs to have access to the widest array of PCR protocols available and thus be able to complete any kind of PCR experiment with ease. The protocols are presented in a step-by-step fashion and cover every aspect of PCR, making this an essential reference for all biological scientists.

PCR Detection of Microbial Pathogens

Автор: Mark Wilks (Ed.)
Название: PCR Detection of Microbial Pathogens
ISBN: 1603273484 ISBN-13(EAN): 9781603273480
Издательство: Springer
Цена: 7167 р.
Наличие на складе: Поставка под заказ.

PCR Detection of Microbial Pathogens

Автор: Mark Wilks (Ed.)
Название: PCR Detection of Microbial Pathogens
ISBN: 1603273522 ISBN-13(EAN): 9781603273527
Издательство: Springer
Рейтинг:
Цена: 15894 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one`s own tests or introducing tests developed by other workers.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Рейтинг:
Цена: 16829 р.
Наличие на складе: Есть (3 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.


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