Автор: Chen Bing-Yuan, Janes Harry W. Название: PCR Cloning Protocols ISBN: 0896039730 ISBN-13(EAN): 9780896039735 Издательство: Springer Рейтинг: Цена: 10280 р. Наличие на складе: Поставка под заказ.
Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Автор: Chen Bing-Yuan, Janes Harry W. Название: PCR Cloning Protocols ISBN: 0896039692 ISBN-13(EAN): 9780896039698 Издательство: Springer Рейтинг: Цена: 17297 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Автор: Rapley Ralph Название: PCR Sequencing Protocols ISBN: 0896033449 ISBN-13(EAN): 9780896033443 Издательство: Springer Цена: 6821 р. Наличие на складе: Поставка под заказ.
Описание: A superb, comprehensive collection of the most reliable protocols for PCR sequencing-all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.
Автор: Walker John M. Название: PCR Protocols on CD (Institutional) ISBN: 1588294099 ISBN-13(EAN): 9781588294098 Издательство: Springer Цена: 22347 р. Наличие на складе: Поставка под заказ.
Описание: In the tradition of the acclaimed Methods in Molecular Biology series, this collection of more than 500 tried-and-tested PCR protocols will become the standard PCR reference set in all biological science laboratories. Comprehensive in scope, yet easily searchable, these protocols will enable PCR labs to have access to the widest array of PCR protocols available and thus be able to complete any kind of PCR experiment with ease. The protocols are presented in a step-by-step fashion and cover every aspect of PCR, making this an essential reference for all biological scientists.
Автор: Meltzer Stephen J Название: PCR in Bioanalysis ISBN: 0896034976 ISBN-13(EAN): 9780896034976 Издательство: Springer Рейтинг: Цена: 13557 р. Наличие на складе: Поставка под заказ.
Описание: PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
Автор: Yuryev Anton Название: PCR Primer Design ISBN: 158829725X ISBN-13(EAN): 9781588297259 Издательство: Springer Рейтинг: Цена: 15894 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
Автор: Theophilus Bimal D. M., Rapley Ralph Название: PCR Mutation Detection Protocols ISBN: 0896036170 ISBN-13(EAN): 9780896036178 Издательство: Springer Цена: 6821 р. Наличие на складе: Поставка под заказ.
Описание: Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.
Описание: Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification.
Автор: Walker John M. Название: PCR Protocols on CD (Personal) ISBN: 1588297136 ISBN-13(EAN): 9781588297136 Издательство: Springer Рейтинг: Цена: 11215 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In the tradition of the acclaimed Methods in Molecular Biology series, this collection of more than 500 tried-and-tested PCR protocols will become the standard PCR reference set in all biological science laboratories. Comprehensive in scope, yet easily searchable, these protocols will enable PCR labs to have access to the widest array of PCR protocols available and thus be able to complete any kind of PCR experiment with ease. The protocols are presented in a step-by-step fashion and cover every aspect of PCR, making this an essential reference for all biological scientists.
Автор: Mark Wilks (Ed.) Название: PCR Detection of Microbial Pathogens ISBN: 1603273484 ISBN-13(EAN): 9781603273480 Издательство: Springer Цена: 7167 р. Наличие на складе: Поставка под заказ.
Автор: Mark Wilks (Ed.) Название: PCR Detection of Microbial Pathogens ISBN: 1603273522 ISBN-13(EAN): 9781603273527 Издательство: Springer Рейтинг: Цена: 15894 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one`s own tests or introducing tests developed by other workers.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 16829 р. Наличие на складе: Есть (3 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
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