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RT-PCR Protocols, Connell Joe



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Цена: 9922р.
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При оформлении заказа до: 26 мар 2021
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Автор: Connell Joe
Название:  RT-PCR Protocols   (Протоколы RT-PCR в реальном времени)
Издательство: Springer
Классификация:
Клеточная биология

ISBN: 0896038750
ISBN-13(EAN): 9780896038752
ISBN: 0-89603-875-0
ISBN-13(EAN): 978-0-89603-875-2
Обложка/Формат: Hardcover
Страницы: 380
Вес: 0.752 кг.
Дата издания: 2002
Серия: Methods in Molecular Biology
Язык: English
Размер: 23.42 x 15.49 x 2.67 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
Дополнительное описание: Формат: 229x152
Илюстрации: 100
Круг читателей: Biomedical researchers and molecular biologists working in university laboratories, hospital research and diagnostic laboratories, and in the biotechnology and pharmaceutical industries
Ключевые слова:
Язык: eng





Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Рейтинг:
Цена: 18809 р.
Наличие на складе: Есть (3 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Recombinant Gene Expression / Reviews and Protocols

Автор: Balbas Paulina, Lorence Argelia
Название: Recombinant Gene Expression / Reviews and Protocols
ISBN: 1588292622 ISBN-13(EAN): 9781588292629
Издательство: Springer
Рейтинг:
Цена: 20899 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Автор: Walker John M.
Название: PCR Protocols on CD (Institutional)
ISBN: 1588294099 ISBN-13(EAN): 9781588294098
Издательство: Springer
Цена: 24976 р.
Наличие на складе: Поставка под заказ.

Описание: In the tradition of the acclaimed Methods in Molecular Biology series, this collection of more than 500 tried-and-tested PCR protocols will become the standard PCR reference set in all biological science laboratories. Comprehensive in scope, yet easily searchable, these protocols will enable PCR labs to have access to the widest array of PCR protocols available and thus be able to complete any kind of PCR experiment with ease. The protocols are presented in a step-by-step fashion and cover every aspect of PCR, making this an essential reference for all biological scientists.

Species Diagnostics Protocols / PCR and Other Nucleic Acid Methods

Автор: Clapp Justin P.
Название: Species Diagnostics Protocols / PCR and Other Nucleic Acid Methods
ISBN: 0896033236 ISBN-13(EAN): 9780896033238
Издательство: Springer
Рейтинг:
Цена: 19331 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification.

PCR Cloning Protocols

Автор: Chen Bing-Yuan, Janes Harry W.
Название: PCR Cloning Protocols
ISBN: 0896039730 ISBN-13(EAN): 9780896039735
Издательство: Springer
Рейтинг:
Цена: 11490 р.
Наличие на складе: Поставка под заказ.

Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

PRINS and In Situ PCR Protocols

Автор: Pellestor Franck
Название: PRINS and In Situ PCR Protocols
ISBN: 1588295494 ISBN-13(EAN): 9781588295491
Издательство: Springer
Рейтинг:
Цена: 14629 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.

PCR Mutation Detection Protocols

Автор: Theophilus Bimal D. M., Rapley Ralph
Название: PCR Mutation Detection Protocols
ISBN: 0896036170 ISBN-13(EAN): 9780896036178
Издательство: Springer
Цена: 7623 р.
Наличие на складе: Поставка под заказ.

Описание: Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

PCR Cloning Protocols

Автор: Chen Bing-Yuan, Janes Harry W.
Название: PCR Cloning Protocols
ISBN: 0896039692 ISBN-13(EAN): 9780896039698
Издательство: Springer
Рейтинг:
Цена: 19331 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

PCR Protocols

Автор: Bartlett John M.S., Stirling David
Название: PCR Protocols
ISBN: 0896036278 ISBN-13(EAN): 9780896036277
Издательство: Springer
Рейтинг:
Цена: 16602 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly since the first edition, and has expanded its use in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The meethods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR techinque in their work.

Автор: Walker John M.
Название: PCR Protocols on CD (Personal)
ISBN: 1588297136 ISBN-13(EAN): 9781588297136
Издательство: Springer
Рейтинг:
Цена: 12535 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: In the tradition of the acclaimed Methods in Molecular Biology series, this collection of more than 500 tried-and-tested PCR protocols will become the standard PCR reference set in all biological science laboratories. Comprehensive in scope, yet easily searchable, these protocols will enable PCR labs to have access to the widest array of PCR protocols available and thus be able to complete any kind of PCR experiment with ease. The protocols are presented in a step-by-step fashion and cover every aspect of PCR, making this an essential reference for all biological scientists.

PCR in Bioanalysis

Автор: Meltzer Stephen J
Название: PCR in Bioanalysis
ISBN: 0896034976 ISBN-13(EAN): 9780896034976
Издательство: Springer
Рейтинг:
Цена: 15151 р.
Наличие на складе: Поставка под заказ.

Описание: PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.

PCR Primer Design

Автор: Yuryev Anton
Название: PCR Primer Design
ISBN: 158829725X ISBN-13(EAN): 9781588297259
Издательство: Springer
Рейтинг:
Цена: 17764 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.


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