Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 11962 р.
Наличие на складе: Есть (3 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Theophilus Bimal D. M., Rapley Ralph
Название: PCR Mutation Detection Protocols
ISBN: 0896036170 ISBN-13(EAN): 9780896036178
Издательство: Springer
Цена: 6018 р.
Наличие на складе: Поставка под заказ.

Описание: Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Автор: Tuan Rocky S.
Название: Recombinant Protein Protocols / Detection and Isolation
ISBN: 089603481X ISBN-13(EAN): 9780896034815
Издательство: Springer
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Цена: 15419 р.
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Описание: Leading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products-recombinant proteins. These state-of-the-art techniques describe a large number of molecular tags and labels, including enzymes, ligand-binding moieties, and immunodetectable molecules. There are also methods to detect interactive proteins and gene expression-mediated alterations in cellular activity, as well as chapters on in situ detection of gene expression. When combined with a companion volume by the same editor, Recombinant Gene Expression Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.

Автор: Pells Steve
Название: Nuclear Reprogramming / Methods and Protocols
ISBN: 1588293793 ISBN-13(EAN): 9781588293794
Издательство: Springer
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Цена: 13612 р.
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Описание: A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.

Автор: Vladimir V. Didenko (Ed.)
Название: In Situ Detection of DNA Damage
ISBN: 1603274081 ISBN-13(EAN): 9781603274081
Издательство: Springer
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Цена: 11962 р.
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Описание: Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.

Автор: Pellestor Franck
Название: PRINS and In Situ PCR Protocols
ISBN: 1588295494 ISBN-13(EAN): 9781588295491
Издательство: Springer
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Цена: 9899 р.
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Описание: This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.

Название: In Situ Hybridization: A Practical Approach
ISBN: 0199636583 ISBN-13(EAN): 9780199636587
Издательство: Oxford Academ
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Цена: 7916 р.
Наличие на складе: Нет в наличии.

Описание: This edition contains protocols detailing the major techniques of "in situ" hybridization in the late 1990s. It includes topics such as: "in situ" hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); and analysis using light and electron microscopes.

Название: Fluorescence in situ hybridization (fish) - application guide
ISBN: 3540705805 ISBN-13(EAN): 9783540705802
Издательство: Springer
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Цена: 12623 р.
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Описание: Provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), which are used to study many aspects of genomic behavior and alterations. This work describes methods using various cell types and tissues as well as different organisms, such as mammalians.

Автор: Polak, Julia M.; McGee, James O`D.
Название: In Situ Hybridization Principles and Practice 2/e (Hardback)
ISBN: 019854880X ISBN-13(EAN): 9780198548805
Издательство: Oxford Academ
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Цена: 16530 р.
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Описание: The technique of in situ hybridisation is vital to molecular biologists and their understanding of the pathophysiology of cellular functions. This practical guide covers all aspects of in situ hybridisation, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology.

Автор: Liehr
Название: Fluorescence In Situ Hybridization (FISH)
ISBN: 3662529572 ISBN-13(EAN): 9783662529577
Издательство: Springer
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Цена: 9074 р.
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Описание: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.

Автор: Copeland William C.
Название: Mitochondrial DNA / Methods and Protocols
ISBN: 0896039722 ISBN-13(EAN): 9780896039728
Издательство: Springer
Цена: 7008 р.
Наличие на складе: Поставка под заказ.

Описание: Internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers.

Автор: Rampal Jang B.
Название: DNA Arrays / Methods and Protocols
ISBN: 089603822X ISBN-13(EAN): 9780896038226
Издательство: Springer
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Цена: 9899 р.
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Описание: In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct DNA microarrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions, these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project.