Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 ), Amini, Shohreh (Editor), White, Martyn K (Editor)

Автор: Andras Perl (Editor)
Название: Autoimmunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1607617196 ISBN-13(EAN): 9781607617198
Издательство: Springer
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Описание: Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.

Автор: Agata Copani; Ferdinando Nicoletti
Название: Cell-Cycle Mechanisms and Neuronal Cell Death
ISBN: 1489998357 ISBN-13(EAN): 9781489998354
Издательство: Springer
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Описание: Cell-Cycle Mechanisms and Neuronal Cell Death examines the role of cell cycle activation in the molecular mechanisms leading to neuronal degeneration. Leading Authors discuss this topic in relation to the major neurological disorders, including Alzheimer’s disease, stroke and epilepsy. This book serves to gain new insights into the molecular determinants of neuronal death and to establish new targets for therapeutic intervention.

Автор: Kerry S. Campbell
Название: Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology, Vol. 612)
ISBN: 1607613611 ISBN-13(EAN): 9781607613619
Издательство: Springer
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Описание: Research has revealed that natural killer (NK) cells are sentinels of the innate immune response. This text offers detailed protocols plus the latest methods and techniques designed to study NK cells within specialized tissue sites in both mice and humans.

Автор: Irving C. Allen (Editor)
Название: Mouse Models of Innate Immunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034803 ISBN-13(EAN): 9781627034807
Издательство: Springer
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Описание: The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense;

Автор: Race L. Kao (Editor)
Название: Cellular Cardiomyoplasty: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627035109 ISBN-13(EAN): 9781627035101
Издательство: Springer
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Описание: In Cellular Cardiomyoplasty: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study cellular cardiomyoplasty, only adult stem cells or adult progenitor cells are included.

Автор: Andrea A. Zachary (Editor), Mary S. Leffell
Название: Transplantation Immunology: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034927 ISBN-13(EAN): 9781627034920
Издательство: Springer
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Описание: PART I: Overview Chapters1. Alloreactivity

Sidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey

2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury

Nicole M. Valenzuela and Elaine F. Reed

3. Cell Mediated Rejection

Kathryn J Wood, Masaaki Zaitsu and Ryoichi Goto

4. Transplantation Tolerance

Colin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg

5. Composite Tissue Transplantation

Gerald Brandacher

6. Transplantation of the Sensitized Patient: Histocompatibility Testing

Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary

7. Thoracic Organ Transplantation: Laboratory Methods

Jignesh K. Patel and Jon A. Kobashigawa

PART II: Methods Chapters

8. HLA Typing by Sequence Specific Primers

Mike Bunce, Biofortuna and Ben Passey, Biofortuna

9. Human Leukocyte Antigen (HLA) Typing By DNA Sequencing

Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley

10. Next-Generation HLA Sequencing using the 454 GS FLX System

Elizabeth A. Trachtenberg and Cherie L. Holcomb

11. Chimerism Testing by Quantitative PCR Using Indel Markers

Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer

12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR)

Raja Rajalingam and Elham Ashouri

13. Complement-dependent Cytotoxicity Crossmatch

Jeremy Ryan Peсa, Donna Fitzpatrick, and Susan L. Saidman

14. Lymphocyte Crossmatching by Flow Cytometry

Robert A. Bray

15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies

John D Smith and Marlene L Rose

16. C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens

Ge Chen and Dolly B. Tyan

17. Tetramer Staining for the Detection of HLA-specific B cells

Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary

18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood

Annette M. Jackson, Donna P. Lucas, Jessica L. Badders

19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation

Duska Dragun

20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA)

Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar

21. Cylex ImmuKnow Cell Function Assay

Adriana Zeevi and John Lunz

22. Detection of Intracellular Cytokines

Nancy L. Reinsmoen and Chih-Hung Lai

23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients

George Vlad and Nicole Suciu-Foca

24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA

Tara K. Sigdel, and Minnie. M. Sarwal

25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing

Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian

Автор: Daryl S. Henderson
Название: DNA Repair Protocols: Eukaryotic Systems (Methods in Molecular Biology)
ISBN: 1468471449 ISBN-13(EAN): 9781468471441
Издательство: Springer
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Автор: Dmitry M. Kolpashchikov (Editor), Yulia V. Gerasim
Название: Nucleic Acid Detection: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627035346 ISBN-13(EAN): 9781627035347
Издательство: Springer
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Описание: This Methods in Molecular Biology book details approaches for the detection of DNA and RNA including recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, step-by-step, reproducible protocols, materials lists, tips and more.

Автор: Susanne M. Bailer (Editor), Diana Lieber (Editor)
Название: Virus-Host Interactions: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627036008 ISBN-13(EAN): 9781627036009
Издательство: Springer
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Описание: This volume covers various aspects of virological research, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level. It features step-by-step, readily reproducible protocols and key implementation advice.

Автор: Kelly M. Fulton (Editor), Susan M. Twine (Editor)
Название: Immunoproteomics: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627035885 ISBN-13(EAN): 9781627035880
Издательство: Springer
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Описание: Providing diverse, up-to-date approaches and techniques for studying the immunoproteome, this Methods in Molecular Biology book offers materials lists, step-by-step reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Автор: Gwyn Davies (Editor)
Название: Vaccine Adjuvants: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1607615843 ISBN-13(EAN): 9781607615842
Издательство: Springer
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Описание: The study of vaccine adjuvants has developed into an exciting area of work. In this authoritative and versatile book, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products.

Автор: Lo Benny K. C.
Название: Antibody Engineering / Methods and Protocols
ISBN: 1588290921 ISBN-13(EAN): 9781588290922
Издательство: Springer
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Описание: A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.