Signal Transduction Immunohistochemistry, Alexander E. Kalyuzhny

Автор: Buchwalow, Igor B. Bocker, Werner
Название: Immunohistochemistry
ISBN: 3642046088 ISBN-13(EAN): 9783642046087
Издательство: Springer
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Описание: This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.

Автор: Kalyuzhny, Alexander E.
Название: Immunohistochemistry Essential Elements and Beyond
ISBN: 3319308912 ISBN-13(EAN): 9783319308913
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Short Historic Overview.- Primary Antibodies.- Choosing Commercial Suppliers of Primary Antibodies.- Detection and Visualization of Antigens.- Ancillary Reagents for IHC.- Preparing Tissues for IHC Staining.- Staining Protocols.- Microscopy Examination and Collection of Digital Images and Their Presentation.- Concluding Remarks.

Автор: Constantine Tsoukas
Название: Lymphocyte Signal Transduction
ISBN: 1489990135 ISBN-13(EAN): 9781489990136
Издательство: Springer
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Цена: 34937.00 р.
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Описание: Signal transduction through leukocyte receptors involves a variety of signaling molecules including kinases, phosphatases, adaptor proteins, small GTPases GTP exchange factors, membrane phospholipids as well as others. These signal transducers, regulated by inter- and intra-molecular interactions, as well as by various post-translational modifications, lead to the activation of transcription factors that mediate cellular differentiation and growth, effector cell functions, and apoptotic cell death. Several investigators from various parts of the world convened at the 3rd Lymphocyte Signal Transduction Workshop in Crete, Greece from May 27 to June 1, 2005 to discuss their most recent findings in leukocyte signaling. This volume represents a collection of topics discussed during the conference.

Автор: Kalyuzhny
Название: Signal Transduction Immunohistochemistry
ISBN: 1617790230 ISBN-13(EAN): 9781617790232
Издательство: Springer
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Описание: Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.  In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC).  Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.