Lymphocyte Signal Transduction, Constantine Tsoukas
Автор: Kalyuzhny Название: Signal Transduction Immunohistochemistry ISBN: 1617790230 ISBN-13(EAN): 9781617790232 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.
Автор: Sudhir Gupta; Frederick W. Alt; Max D. Cooper; Fri Название: Mechanisms of Lymphocyte Activation and Immune Regulation XI ISBN: 1441942831 ISBN-13(EAN): 9781441942838 Издательство: Springer Рейтинг: Цена: 26120.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In recent years, major developments have increased understanding of various genetic and epigenetic regulatory processes that are critical for the generation of B cell repertoires.
Автор: Cornelis Murre Название: Epigenetic Regulation of Lymphocyte Development ISBN: 3642447678 ISBN-13(EAN): 9783642447679 Издательство: Springer Рейтинг: Цена: 32004.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The studies described in this volume serve as a starting point to familiarize one self with the multifarious differences in epigenetic designs that orchestrate the progression of developing blood cells. They also may serve as a general paradigm for the mechanisms that underpin the control of eukaryotic gene expression.
Автор: Alexander E. Kalyuzhny Название: Signal Transduction Immunohistochemistry ISBN: 1493967576 ISBN-13(EAN): 9781493967575 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications Kathy Brumbaugh, Wen-Chie Liao, J. P. Houchins, Jeff Cooper, and Steve Stoesz
2. Antibodies That Work Again and Again and Again Kameron Simpson and Michael Browning 3. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods Biji T. Kurien and R. Hal Scofield 4. Acquisition and Post-Processing of Immunohistochemical Images Jerry Sedgewick 5. Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction Jessica Kalra and Jennifer Baker 6. EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis Christianne Bandeira-Melo, Ligia Almeida Paiva, Natбlia R. T. Amorim, Peter F. Weller, and Patricia T. Bozza 7. Preservation of Specific Protein Signaling States using Heat Based Stabilizor System Mats Borйn 8. Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species Jodi Hagen, David Schwartz, and Alexander E. Kalyuzhny 9. Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages Charles A. Warwick and Yuriy M. Usachev 10. Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines and Surface Markers Chris Goetz, Li-Jen Peng, Birte Aggeler, and Jody Bonnevier 11. A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy Aishwarya Sathyanarayan 12. Apoptotic Bodies: Selective Detection in Extracellular Vesicles Paul Hauser, Sha Wang, and Vladimir V. Didenko 13. Activation and Differentiation of Mesenchymal Stem Cells Pravin J. Mishra and Debabrata Banerjee 14. High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP Michael Grahek, Ana Ptak, and Alexander E. Kalyuzhny 15. Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry David Schwartz, Michael Grahek, Yingwei He, Wei Wang, Jennifer Nguyen, and Alexander E. Kalyuzhny 16. Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D Candace C. Minchew and Vladimir V. Didenko 17. Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment Jessica Kalra and Jennifer Baker 18. Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors Jacob Ricca, Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan, Matthew Brendel, Dmitriy Zamarin, Jedd D. Wolchok, and Katia Manova-Todorova 19. Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan, Matthew Brendel, and Katia Manova-Todorova 20. Automated High Content Screening of g--H2AX Expression in HeLa Cells Nate Hopp, Jodi Hagen, Birte Aggeler, and Alexander E. Kalyuzhny
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