Adeno-Associated Virus (AAV) Vectors in Gene Therapy, Kenneth I. Berns; Catherine Giraud

Автор: Merten
Название: Viral Vectors for Gene Therapy
ISBN: 161779094X ISBN-13(EAN): 9781617790942
Издательство: Springer
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Цена: 22359.00 р.
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Описание: The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

Автор: Candiani
Название: Non-Viral Gene Delivery Vectors
ISBN: 1493937162 ISBN-13(EAN): 9781493937165
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery—also termed reversetransfection—and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods andProtocols is written for experimentalists, and is an essential part of manylaboratory bookshelves. This book will help novice and professionals alikesucceed in their research in this field.

Автор: Sven Klimpel; Heinz Mehlhorn
Название: Bats (Chiroptera) as Vectors of Diseases and Parasites
ISBN: 3642393322 ISBN-13(EAN): 9783642393327
Издательство: Springer
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Цена: 23508.00 р.
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Описание: Bats (Chiroptera) as Vectors of Diseases and Parasites

Автор: Kenneth Palmer; Yuri Gleba
Название: Plant Viral Vectors
ISBN: 3642408281 ISBN-13(EAN): 9783642408281
Издательство: Springer
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Цена: 26122.00 р.
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Описание: This wide-ranging review shows how far our understanding of plant viral vectors has come, with experts in `vectorology` contributing chapters on their work applying the science to cutting-edge techniques in genetics, nanotechnology, and other fields.

Автор: Didier Trono
Название: Lentiviral Vectors
ISBN: 364262667X ISBN-13(EAN): 9783642626678
Издательство: Springer
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Цена: 18167.00 р.
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Описание: For the first time a compilation of chapters that depict the biological bases underlying the development of lentiviral vectors, the techniques involved in the manufacture of this new gene delivery tool, and its most promising applications.