Описание: The use of supercritical fluids in analytical chemistry is still grow- ing. The advantages of supercritical CO , such as having dif- 2 fusivity like a gas and solvating power depending on temperature and pressure, are also valid for other fluids and modified fluids.
Автор: Bailon Pascal Название: Affinity Chromatography / Methods and Protocols ISBN: 0896036944 ISBN-13(EAN): 9780896036949 Издательство: Springer Цена: 7540.00 р. Наличие на складе: Поставка под заказ.
Автор: Walls Название: Protein Chromatography ISBN: 1607619121 ISBN-13(EAN): 9781607619123 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Автор: Luck Martin Название: Hormones: A Very Short Introduction ISBN: 0199672873 ISBN-13(EAN): 9780199672875 Издательство: Oxford Education Рейтинг: Цена: от 1138.00 р. Наличие на складе: Есть
Описание: Hormones are vital to the workings of the body, and while many people are aware of what hormones do, few have an appreciation of the nature and importance of the endocrine system. In this Very Short Introduction, Martin Luck explains what hormones are, what they do, where they come from, and how they work.
Автор: Andrew Kenney; Susan Fowell Название: Practical Protein Chromatography ISBN: 1489940162 ISBN-13(EAN): 9781489940162 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 0ne 0fthe many 1mpact5 0frec0m61nant DNA techn0109y 0ver the 1a5t 15 year5 ha5 6een a 5tr0n91y refre5hed 1ntere5t 1n meth0d5 f0r the 5eparat10n and pur1f1cat10n 0f pr0te1n5.7h15 1ntere5t ha5 enc- pa55ed n0t 0n1y ana1yt1ca1 5eparat10n5, 6ut a150 5ma11- and 1ar9e-5ca1e preparat1ve meth0d5 d1rected t0 60th pure and app11ed re5earch thr0u9h0ut 610109y and 610med1c1ne. Many 0f the new 0r 5u65tant1a11y m0d1f1ed techn14ue5 deve1- 0ped have 6een rep0rted 1n the 11terature, 6ut a 5uff1c1ency 0f deta11ed pract1ca1 he1p 1n e5ta6115h1n9 the5e meth0d5 f0r the f1r5t t1me 1n a new 1a60rat0ry ha5 0ften 6een d1ff1cu1t t0 f1nd. W1th the5e pr061em5 1n m1nd, we expect that Pract1ca1Pr0te1n Chr0mat09raphy, de519ned a5 a key v01ume 1n the Meth0d5 1n M01ecu1ar 810109y 5er1e5, w111 pr0v1de c0nc15e pract1ca1 he1p t0 th05e carry1n9 0ut new techn14ue5 f0r the f1r5t t1me. Each chapter ha5 6een wr1tten 6y expert auth0r5 kn0wn t0 have d1rect and re9u1ar pract1ca1 exper1ence w1th the1r ch05en techn14ue5. 7he 5tructure 0feach chapter 15 de519ned t0 make 1t ea5y f0r a w0rker new t0 the meth0d t0 f0110w 1t t0 an effect1ve c0nc1u510n. An 1nt- duct10n treat5 the the0ry 6eh1nd the meth0d 6e1n9 de5cr16ed. 7he Mater1a15 and Meth0d5 5ect10n5 a110w the reader t0 prepare f0r, and then perf0rm techn14ue5 1n a rat10na1 5tepw15e manner. 7he N0te5 5ect10n5 pr0v1de the 50rt 0f6ack9r0und h1nt5 and tr1ck5 that are 50 0ften e55ent1a1 f0r 5ucce55, 6ut are rare1y rep0rted 1n the 11terature.
Автор: Heinz Engelhardt; K. Aitzetm?ller; J. Asshauer; P. Название: Practice of High Performance Liquid Chromatography ISBN: 3642692273 ISBN-13(EAN): 9783642692277 Издательство: Springer Рейтинг: Цена: 14365.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: During its short 20 year history High Performance Liquid Chro- matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis.
Автор: Alfred L. Yergey; Charles G. Edmonds; Ivor A.S. Le Название: Liquid Chromatography/Mass Spectrometry ISBN: 1489936076 ISBN-13(EAN): 9781489936073 Издательство: Springer Рейтинг: Цена: 19591.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
