Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences, Julian E. Beesley

Автор: Ganjei-Azar
Название: Color Atlas of Immunocytochemistry in Diagnostic Cytology
ISBN: 0387321217 ISBN-13(EAN): 9780387321219
Издательство: Springer
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Описание: This atlas examines the benefits and limitations of immunocytochemistry (ICC) in contemporary cytology. This practical handbook will allow for quick reference in the selection and interpretation of markers in specific differential diagnostic in the daily practice of diagnostic cytology.

Автор: Burry, Richard W.
Название: Immunocytochemistry: Practical Guide for Biomedical Research
ISBN: 1441913033 ISBN-13(EAN): 9781441913036
Издательство: Springer
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Описание: Due to the dramatic increase in productivity in biomedical research, immunocytochemistry has emerged as a major technique. This book is the first practical guide to planning, performing and evaluating immunocytochemistry experiments.

Автор: Richard W. Burry
Название: Immunocytochemistry
ISBN: 1489984429 ISBN-13(EAN): 9781489984425
Издательство: Springer
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Цена: 9083.00 р.
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Описание: Description: In biomedical research, because of a dramatic increase in productivity, immunocytochemistry has emerged as a major technique. The proposed book will provide the first practical guide to planning, performing, and evaluating immunocytochemical experiments. In today’s graduate education the emphasis is on doing research and not on formal class work. Graduate students therefore lack the background in many essential techniques necessary to perform research in fields in which they were not trained. As director of a university core microscopy facility which sees students and faculty from dozens of laboratories each year, Dr. Burry has surmised the vast majority of these novice microscope users need considerable help. In an attempt to educate users, Dr. Burry has initiated immunocytochemistry seminars and workshops which serve to train people in this powerful research tool. The proposed book is an outgrowth of these presentations and conversations with, by now, hundreds of people who have asked for help. The philosophy which separates this book from other books in this field is that it is practical, rather than academic. In looking at other important immunocytochemistry titles, the predominant orientation is academic, with the author attempting to comprehensively discuss the topic. For example, one book with sample preparation lists ten fixatives which can be used; however, only two such fixatives are commonly used today. In this particular title, the detailed discussion of old methods might be seen as important in establishing the author as an expert. By contrast, the approach for Burry’s book would be to discuss methods based on what works in animal research laboratories today, and focus only on the most productive methods. An additional distinction with this proposed book is the focus on animal research and not human pathology. There is a certification program for pathology technicians which requires them to learn a set body of material based on processing human tissue for examination by a pathologist. Many of the books on immunocytochemistry aim at this large pathology user base. Due to historical reasons, pathology laboratories process human tissues in a specific way and embed the tissue in paraffin, as has been done for over a century. In the last ten years, the power of immunocytochemistry in clinical diagnosis has become clear and has accordingly been adapted to pathology. However, the extensive processing needed for paraffin sections is not needed if the tissues are from research animals. Processing for animal-based tissues takes about a third of the time and results in higher quality images. The focus of this book is on processing these animal research tissues for immunocytochemistry. Today, there are no technique books which are aimed at this user base. As a subject matter expert in the area of the proposed book, Dr. Burry will make recommendations and offer opinions. Because this field is new and is emerging, there are numerous advantages of specific methods over other, more generalized methods. The purpose of this book is to show a novice how to do immunocytochemistry without engaging in a discussion of possible advanced methods. For the advanced user, there are several good books which discuss the unusual methods, yet for the novice there are currently none. Main Author : Richard W. Burry, The Ohio State University (United States). The Outline of the Book : Each chapter supplies a set of important principals and steps necessary for good immunocytochemistry. The information is distilled down to include only the most important points and does not attempt to cover infrequently used procedures or reagents. At the end of most chapters is a section on trouble-shooting many of the common problems using the Sherlock Holmes method. Each chapter also includes specific protocols which can be used. The goal of each chapter is to present the reader with enough information to successfully design experiments and solve many

Автор: Boye Schnack Nielsen
Название: In Situ Hybridization Protocols
ISBN: 1493955268 ISBN-13(EAN): 9781493955268
Издательство: Springer
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Описание: In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules.

Автор: Gareth Griffiths; B. Burke; J. Lucocq
Название: Fine Structure Immunocytochemistry
ISBN: 3642770975 ISBN-13(EAN): 9783642770975
Издательство: Springer
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Цена: 15672.00 р.
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Описание: Admittedly, many of the processes involved, such as those required to embed tissue in epoxy resins, involve multiple interconnected steps, which make it difficult to follow the details of anyone of these steps.

Автор: George Uhl
Название: In Situ Hybridization in Brain
ISBN: 146159488X ISBN-13(EAN): 9781461594888
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain.

Автор: Ian A. Darby; Tim D. Hewitson
Название: In Situ Hybridization Protocols
ISBN: 1617375446 ISBN-13(EAN): 9781617375446
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Emphasizes tissue and cell in situ hybridization methods. In this title, among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. It includes protocols that follow the successful "Methods in Molecular Biologya" series format.

Автор: Adalberto Merighi; Laura Lossi
Название: Immunocytochemistry and Related Techniques
ISBN: 1493923129 ISBN-13(EAN): 9781493923120
Издательство: Springer
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Цена: 20962.00 р.
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Описание: Immunocytochemistry and Related Techniques