In Situ Hybridization Protocols, Ian A. Darby; Tim D. Hewitson
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Описание: The restrictions on base pairing- A pairing with T (U), and G pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcription.
Автор: G. Kalloo; J.B. Chowdhury Название: Distant Hybridization of Crop Plants ISBN: 3642843085 ISBN-13(EAN): 9783642843082 Издательство: Springer Рейтинг: Цена: 18284.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In most of the cases, backcross or modified backcross methods have been followed to utilize wild species, and thus numerous types of resistance and other economical attributes have been transferred in the recurrent parents.
Автор: Franck Pellestor Название: PRINS and In Situ PCR Protocols ISBN: 1617376361 ISBN-13(EAN): 9781617376368 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
Автор: Vladimir V. Didenko Название: In Situ Detection of DNA Damage ISBN: 1617372722 ISBN-13(EAN): 9781617372728 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning "on site" or "in place." It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning "in situ" is used as part of the terms "in situ PCR," "in situ transcription," "in situ hybridization," "in situ end labeling," and "in situ ligation." Sometimes the "in situ" term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.
Автор: Jiang Gu Название: In Situ PCR and Related Technology ISBN: 1468468456 ISBN-13(EAN): 9781468468458 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology.
Автор: Liehr Название: Fluorescence In Situ Hybridization (FISH) ISBN: 3662529572 ISBN-13(EAN): 9783662529577 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.
Автор: G.R. Coulton; J. de Belleroche Название: In Situ Hybridization: Medical Applications ISBN: 0792389891 ISBN-13(EAN): 9780792389897 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In situ hybridization has developed as a means of localizing specific DNA and RNA sequences within tissues. This book describes theoretical and practical aspects of the technology. It explains the contribution made by in situ hybridization to specific areas of medical research.
Автор: Ephrussi Boris Название: Hybridization of Somatic Cells ISBN: 0691619468 ISBN-13(EAN): 9780691619460 Издательство: Wiley Рейтинг: Цена: 5544.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Since its discovery in I960, the hybridization of somatic cells has evolved from a biological curiosity into an analytical method that today underlies nearly all investigations of the genetic aspects of various biological phenomena. As an eyewitness to this development from its inception forward, Boris Ephrussi here relates the history of somatic h
Автор: Toshiyuki Nagata; Y.P.S. Bajaj Название: Somatic Hybridization in Crop Improvement II ISBN: 3642625002 ISBN-13(EAN): 9783642625008 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This richly illustrated volume describes how somatic hybrids can contribute to the improvement of crops. It comprises 24 chapters dealing with interspecific and intergeneric somatic hybridization and cybridization, providing valuable tools for plant breeders.
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