PRINS and In Situ PCR Protocols, Franck Pellestor

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Ian A. Darby; Tim D. Hewitson
Название: In Situ Hybridization Protocols
ISBN: 1617375446 ISBN-13(EAN): 9781617375446
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Emphasizes tissue and cell in situ hybridization methods. In this title, among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. It includes protocols that follow the successful "Methods in Molecular Biologya" series format.

Автор: Ralph Rapley
Название: PCR Sequencing Protocols
ISBN: 1489940383 ISBN-13(EAN): 9781489940384
Издательство: Springer
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Цена: 16979.00 р.
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Описание: Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

Автор: Jiang Gu
Название: In Situ PCR and Related Technology
ISBN: 1468468456 ISBN-13(EAN): 9781468468458
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology.

Автор: Bing-Yuan Chen; Harry W. Janes
Название: PCR Cloning Protocols
ISBN: 1617372811 ISBN-13(EAN): 9781617372810
Издательство: Springer
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Цена: 25853.00 р.
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Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White`s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.

Автор: Connell Joe
Название: RT-PCR Protocols
ISBN: 0896038750 ISBN-13(EAN): 9780896038752
Издательство: Springer
Цена: 13270.00 р.
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Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.