In Situ Detection of DNA Damage, Vladimir V. Didenko

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Ian A. Darby; Tim D. Hewitson
Название: In Situ Hybridization Protocols
ISBN: 1617375446 ISBN-13(EAN): 9781617375446
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Emphasizes tissue and cell in situ hybridization methods. In this title, among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. It includes protocols that follow the successful "Methods in Molecular Biologya" series format.

Автор: G.R. Coulton; J. de Belleroche
Название: In Situ Hybridization: Medical Applications
ISBN: 0792389891 ISBN-13(EAN): 9780792389897
Издательство: Springer
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Цена: 23058.00 р.
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Описание: In situ hybridization has developed as a means of localizing specific DNA and RNA sequences within tissues. This book describes theoretical and practical aspects of the technology. It explains the contribution made by in situ hybridization to specific areas of medical research.

Автор: Jiang Gu
Название: In Situ PCR and Related Technology
ISBN: 1468468456 ISBN-13(EAN): 9781468468458
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology.

Автор: Liehr
Название: Fluorescence In Situ Hybridization (FISH)
ISBN: 3662529572 ISBN-13(EAN): 9783662529577
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.

Автор: Franck Pellestor
Название: PRINS and In Situ PCR Protocols
ISBN: 1617376361 ISBN-13(EAN): 9781617376368
Издательство: Springer
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Цена: 16769.00 р.
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Описание: Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.

Автор: Zhiguo Wang; Baofeng Yang
Название: MicroRNA Expression Detection Methods
ISBN: 3642425186 ISBN-13(EAN): 9783642425189
Издательство: Springer
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Цена: 28732.00 р.
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Описание: This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression as well as their application in miRNA research and their potential as tools for clinical diagnosis and prognosis.