MicroRNA Expression Detection Methods, Zhiguo Wang; Baofeng Yang

Автор: Zhang
Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
ISBN: 1607617684 ISBN-13(EAN): 9781607617686
Издательство: Springer
Рейтинг:
Цена: 22359.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.

Автор: Xueji Zhang; Haifeng Dong; Yaping Tian
Название: MicroRNA Detection and Pathological Functions
ISBN: 3662472929 ISBN-13(EAN): 9783662472927
Издательство: Springer
Рейтинг:
Цена: 7685.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.

Автор: Singh Shree Ram
Название: MicroRNA in Development and in the Progression of Cancer
ISBN: 1489980644 ISBN-13(EAN): 9781489980649
Издательство: Springer
Рейтинг:
Цена: 23757.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: miRNAs are a class of endogenous, small non-protein coding RNA molecules (~ 22 nucleotides) which are novel post-transcriptional regulators of gene expression. Since we have hundreds of miRNAs, the major challenge is now to understand their specific biological function. In fact the experimental evidence suggests that signaling pathways could be ideal candidates for miRNA-mediated regulation. Several studies suggest that miRNAs affect the responsiveness of cells to signaling molecules such as WNT, Notch, TGF-? and EGFR. Altered expression of particular miRNAs has been implicated in the onset and development of cancer and could be used as potential biomarkers for the disease. Recently, many studies have found miRNAs have crucial regulatory roles in Cancer stem cells (CSCs) a kind of tumor initiating cells (TICs) and dormancy. Findings also suggest that DNA methylation may be important in regulating the expression of many miRNAs in several cancer initiating cells. Several miRNAs are known to either upregulated or downregulated in CSCs when compared to non-cancerous cells from the same tissues. CSCs are a small subpopulation of cells identified in a variety of tumors and involve in self-renewal, differentiation, chemoresistance and tumorigenesis. The volume will give a comprehensive account of important advancements in the area of miRNAs and cancer.

Автор: Rani
Название: MicroRNA Profiling
ISBN: 1493965220 ISBN-13(EAN): 9781493965229
Издательство: Springer
Рейтинг:
Цена: 18167.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

Автор: Shao-Yao Ying
Название: MicroRNA Protocols
ISBN: 1493976001 ISBN-13(EAN): 9781493976003
Издательство: Springer
Рейтинг:
Цена: 16769.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание:

This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that's cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource that provides researchers with a comprehensive study of miRNAs and its numerous components and uses.

Автор: Suresh Alahari
Название: MicroRNA in Cancer
ISBN: 9401784930 ISBN-13(EAN): 9789401784931
Издательство: Springer
Рейтинг:
Цена: 15672.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book explores the rapidly emerging field of microRNA biology, highlighting the importance of microRNAs in cancer. This first reference of its kind will benefit working scientists in cancer biology, as well as advanced undergraduate and graduate students.

Автор: Jian-Bing Fan
Название: Next-Generation MicroRNA Expression Profiling Technology
ISBN: 1493962175 ISBN-13(EAN): 9781493962174
Издательство: Springer
Рейтинг:
Цена: 20263.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: The rapid pace of research in this field continues to drive technical advances that make methods more sensitive and applicable. This volume features many new methodologies described by leading researchers, including reproducible laboratory protocols.

Автор: Tamas Dalmay
Название: MicroRNA Detection and Target Identification
ISBN: 1493968645 ISBN-13(EAN): 9781493968640
Издательство: Springer
Рейтинг:
Цена: 20962.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This collection of miRNA detection and target identification protocols details new developments in traditional detection such as northern blot, quantitative real-time PCR and in situ hybridization, and guides through novel approaches such as nanotechnology, microfluidics, based detection methods, miRNAs as biomarkers and others.

Автор: Fazlul H. Sarkar
Название: MicroRNA Targeted Cancer Therapy
ISBN: 3319051334 ISBN-13(EAN): 9783319051338
Издательство: Springer
Рейтинг:
Цена: 23508.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: The most attractive feature of miRNAs is that one miRNA can regulate many target genes (mRNAs) and thus miRNA targeted therapy is highly promising because multiple genes could be regulated by targeting a single miRNA, which becomes very important for the killing of highly heterogeneous populations of cancer cells within a tumor mass.