Recombinant Protein Protocols, Rocky S. Tuan

Автор: Balbas Paulina, Lorence Argelia
Название: Recombinant Gene Expression / Reviews and Protocols
ISBN: 1588292622 ISBN-13(EAN): 9781588292629
Издательство: Springer
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Цена: 27950.00 р.
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Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Автор: Paulina Balbas; Argelia Lorence
Название: Recombinant Gene Expression
ISBN: 1617374601 ISBN-13(EAN): 9781617374609
Издательство: Springer
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Цена: 23058.00 р.
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Описание: Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on-gene expression.

Автор: Amanda S. Coutts
Название: Adhesion Protein Protocols
ISBN: 1627035370 ISBN-13(EAN): 9781627035378
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This book covers techniques in cellular adhesion. It features lists of the necessary materials and reagents; step-by-step, readily reproducible protocols; and notes on troubleshooting and avoiding known pitfalls.

Автор: Andrew F. Hill
Название: Prion Protein Protocols
ISBN: 1617378313 ISBN-13(EAN): 9781617378317
Издательство: Springer
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Цена: 18167.00 р.
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Описание: While much information has been gained regarding the molecular nature of prion proteins, only recently have several research groups begun to cast light on turning the normal prion protein into its aberrant, infectious form.

Автор: Ren-Jang Lin
Название: RNA-Protein Interaction Protocols
ISBN: 1617375551 ISBN-13(EAN): 9781617375552
Издательство: Springer
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Цена: 23058.00 р.
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Описание: This updated and expanded second edition examines cutting-edge techniques developed recently, along with tried-and-tested methods. Expert contributors explore the isolation and characterization of RNA-protein complexes and related novel techniques.

Автор: Yawen Bai
Название: Protein Folding Protocols
ISBN: 1617376760 ISBN-13(EAN): 9781617376764
Издательство: Springer
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Цена: 20263.00 р.
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Описание: Covering experiment and theory, bioinformatics approaches, and state-of-the-art simulation protocols for better sampling of the conformational space, this volume describes a broad range of techniques to study, predict, and analyze the protein folding process.

Автор: MГјller Kristian, Arndt Katja
Название: Protein Engineering Protocols
ISBN: 1588290727 ISBN-13(EAN): 9781588290724
Издательство: Springer
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Цена: 25853.00 р.
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Описание: Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering. The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented.Following the successful Methods in Molecular Biologyв„ў series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators.