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Protein Engineering Protocols, Müller Kristian, Arndt Katja



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: 30 2022
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: Müller Kristian, Arndt Katja
 Protein Engineering Protocols
ISBN: 9781588290724
: Springer
:

ISBN-10: 1588290727
/: Hardback
: 328
: 0.724 .
: 15.09.2006
: Methods in Molecular Biology
: English
: 64 black & white illustrations, 1 colour illustrations, biography
: 235 x 158 x 28
: Professional & vocational
: Link
:
:
: : 229x152
: 64
: Biologists, biotechnologists, biochemists, chemists, molecular and cell biologists
:
: eng





Principles of Tissue Engineering,

: Robert Lanza
: Principles of Tissue Engineering,
ISBN: 0123983584 ISBN-13(EAN): 9780123983589
: Elsevier Science
:
: 17556 . 21945.00 -20%
  : (2 .)
: Now in its fourth edition, Principles of Tissue Engineering has been the definite resource in the field of tissue engineering for more than a decade. The fourth edition provides an update on this rapidly progressing field, combining the prerequisites for a general understanding of tissue growth and development, the tools and theoretical information needed to design tissues and organs, as well as a presentation by the worlds experts of what is currently known about each specific organ system. As in previous editions, this book creates a comprehensive work that strikes a balance among the diversity of subjects that are related to tissue engineering, including biology, chemistry, material science, and engineering, among others, while also emphasizing those research areas that are likely to be of clinical value in the future. . This edition includes greatly expanded focus on stem cells, including induced pluripotent stem (iPS) cells, stem cell niches, and blood components from stem cells. This research has already produced applications in disease modeling, toxicity testing, drug development, and clinical therapies. This up-to-date coverage of stem cell biology and other emerging technologies such as brain-machine interfaces for controlling bionics and neuroprostheses is complemented by a series of new and updated chapters on recent clinical experience in applying tissue engineering, as well as a new section on the application of tissue-engineering techniques for food production. The result is a comprehensive textbook that will be useful to students and experts alike.

Protein Purification Protocols

: Cutler Paul
: Protein Purification Protocols
ISBN: 1588290670 ISBN-13(EAN): 9781588290670
: Springer
:
: 23099 .
  : .

: This new edition of Protein Purification Protocols (1996) completely updates the existing protocols to reflect recent advances and adds the enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins.

Recombinant Protein Protocols / Detection and Isolation

: Tuan Rocky S.
: Recombinant Protein Protocols / Detection and Isolation
ISBN: 0896034003 ISBN-13(EAN): 9780896034006
: Springer
:
: 9234 .
  : .

: Leading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products-recombinant proteins. These state-of-the-art techniques describe a large number of molecular tags and labels, including enzymes, ligand-binding moieties, and immunodetectable molecules. There are also methods to detect interactive proteins and gene expression-mediated alterations in cellular activity, as well as chapters on in situ detection of gene expression. When combined with a companion volume by the same editor, Recombinant Gene Expression Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.

The Protein Protocols Handbook

: Walker John M.
: The Protein Protocols Handbook
ISBN: 0896039412 ISBN-13(EAN): 9780896039414
: Springer
:
: 11544 .
  : .

: This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover with many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls and on modifying the method to function within your own experimental circumstances.

Protein Kinase C Protocols

: Newton Alexandra C.
: Protein Kinase C Protocols
ISBN: 1588290689 ISBN-13(EAN): 9781588290687
: Springer
:
: 19056 .
  : .

: The first compilation of classic and emerging laboratory techniques for the study of the biochemistry and cell biology of protein kinase C (PKC). Described in step-by-step detail, these methods can be easily used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C.. Each protocol is introduced in the context of PKC function and regulation and contains many notes on how best to deal with the problems that may occur. Comprehensive and authoritative, Protein Kinase C Protocols is a timely compilation of biophysical, biochemical, cell biological, and molecular biological approaches that brings protein kinase C research into any laboratory interested in studying it.

Antibody Engineering / Methods and Protocols

: Lo Benny K. C.
: Antibody Engineering / Methods and Protocols
ISBN: 1588290921 ISBN-13(EAN): 9781588290922
: Springer
:
: 20211 .
  : .

: A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.

Lentivirus Gene Engineering Protocols

: Federico Maurizio
: Lentivirus Gene Engineering Protocols
ISBN: 1607615320 ISBN-13(EAN): 9781607615323
: Springer
:
: 13859 .
  : .

: Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition, covers the most relevant issues and techniques of LV-based gene engineering. This practical guide is aimed at those who wish to know more about this vital area of study.

Analytics of ProteinDNA Interactions (Advances in Biochemical Engineering/Biotechnology)

: Harald Seitz
: Analytics of ProteinDNA Interactions (Advances in Biochemical Engineering/Biotechnology)
ISBN: 3642080073 ISBN-13(EAN): 9783642080074
: Springer
:
: 36845 .
  : .

: With contributions by numerous experts

Protein Engineering and Design

: Park
: Protein Engineering and Design
ISBN: 1420076582 ISBN-13(EAN): 9781420076585
: Taylor&Francis
:
: 23375 .
  : .

: The emerging use of the computational design approach as a means of engineering proteins with novel functions has led to widespread usage of computational analysis in protein engineering at large. However, because the structure and function of protein molecules are coupled at the molecular level, many critical questions are left unanswered and many of the intricate design rules unclear. This book de-mystifies computational analysis by providing a lucid description of computations role in protein engineering and tracing the history of protein design.

Protein Engineering Techniques

: Krishna Mohan Poluri; Khushboo Gulati
: Protein Engineering Techniques
ISBN: 9811027315 ISBN-13(EAN): 9789811027314
: Springer
:
: 6351 .
  : .

: This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.

Oxidative Stress Biomarkers and Antioxidant Protocols

: Armstrong Donald
: Oxidative Stress Biomarkers and Antioxidant Protocols
ISBN: 0896038505 ISBN-13(EAN): 9780896038509
: Springer
:
: 17901 .
  : .

: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Regulatory Protein Modification / Techniques and Protocols

: Hemmings Hugh C.
: Regulatory Protein Modification / Techniques and Protocols
ISBN: 0896034151 ISBN-13(EAN): 9780896034150
: Springer
:
: 21366 .
  : .

: Part I. Introduction. Recombinant Gene Expression Protocols: Overview of Experimental Strategies for the Expression of Recombinant Genes, Rocky S. Tuan. Part II. Expression in E. coli. Designing Expression Plasmid Vectors in E. coli, Paulina Balbás. Design of Bacterial Hosts for lac-Based Expression Vectors, Herbert P. Schweizer and RoxAnn R. Karkhoff-Schweizer. Bacteriophage l-Based Expression Vectors, Alan C. Christensen. Strategies for Gene Fusions, Stefan Ståhl, Per-Åke Nygren, and Mathias Uhlén. Application of the E. coli trp Promoter, Daniel G. Yansura and Steven H. Bass. Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7, George A. Garcia and Shaorong Chong. Expression and Secretion of Proteins in E. coli, Ophry Pines and Masayori Inouye. Synthetic Two-Cistron Expression System, Brigitte E. Schoner. Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translation, Christopher Coleclough. Part III. Expression in Yeast. Yeast Plasmids, Keshav K. Singh and Jack A. Heinemann. Inducible Expression Cassettes in Yeast: GAL4, Lawrence M. Mylin and James E. Hopper. Inducible Expression Cassettes in Yeast: ADH2, Virginia L. Price. Constitutive Expression Vectors: PGK, Ian R. Graham and Alistair Chambers. Part IV. Viral Expression Systems. Design and Construction of Recombinant Vaccinia Viruses, Christopher C. Broder and Patricia L. Earl. A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus, Haifeng Chen and R. Padmanabhan. Design of Retroviral Expression Vectors, Kenine E. Comstock, Nathaniel F. Watson, and John C. Olsen. Use of Defective Herpes-Derived Plasmid Vectors, Filip Lim, Dean Hartley, Philip Starr, Song Song, Phung Lang, Linda Yu, Yaming Wang, and Alfred I. Geller. Part V. Expression in Nonmammalian Higher Eukaryotes. Use of Baculovirus Expression Vectors, David R. O'Reilly. Expression of Exogenous Genes in Xenopus Oocytes, Eggs, and Embryos, Koichiro Shiokawa, Chie Koga, Yuzuru Ito, and Mikihito Shibata. Analysis of Heterologous Gene Expression in Xenopus Blastomeres, Sally A. Moody. Part VI. Expression in Mammalian Cells. Overview of Vector Design for Mammalian Gene Expression, Randal J. Kaufman. Experimental Strategies in Efficient Transfection of Mammalian Cells: Calcium Phosphate and DEAE-Dextran, Gregory S. Pari and Wayne A. Keown. Experimental Strategies in Efficient Transfection of Mammalian Cells: Electroporation, Donald C. Chang. Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer, Rémy A. Aubin, Michael Weinfeld, Marzieh Taghavi, Razmik Mirzayans, and Malcolm C. Paterson. Selection of Transfected Cells: Magnetic Affinity Cell Sorting, Raji Padmanabhan, Snorri S. Thorgeirsson, and R. Padmanabhan. Selection of Transfected Cells and Coamplification of Transfected Genes, Susan E. Kane. Optimization of Growth, Viability, and Specific Productivity for Expression of Recombinant Proteins in Mammalian Cells, Jennie P. Mather, Alison Moore, and Robert Shawley. Part VII. Expression in Transgenic Organisms. Promoters to Express Cloned Genes Uniformly in Drosophila, Jean-Paul Vincent and Charles Girdham. Application of Micromechanical Piercing Structures for Genetic Transformation of Nematodes, Sarwar Hashmi and Randy Gaugler. Strategies in Generating Transgenic Mammals, Olena Jacenko. Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens, Nathalie Allioli, Gérard Verdier, and Catherine Legras. Strategies for the Production of Transgenic Chickens, Robert J. Etches and Ann M. Verrinder Gibbins. Part VIII. Expression in Plants. Plant Cell Transfection by Electroporation, William B. Terzaghi and Anthony R. Cashmore. Transformation and Foreign Gene Expression in Plants Mediated by Microprojectile Bombardment, Henry Daniell. Transformation of the Cereals Using Agrobacterium, Jean Gould. Transformation of Microalgae Using Silicon Carbide Whiskers, Terri G. Dunahay, Sally A. Adler, and Jonathan W. Jarvik. Index.


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