Автор: Shelley D. Minteer Название: Enzyme Stabilization and Immobilization ISBN: 1493964976 ISBN-13(EAN): 9781493964970 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).
Автор: Pratyoosh Shukla; Brett I. Pletschke Название: Advances in Enzyme Biotechnology ISBN: 813221093X ISBN-13(EAN): 9788132210931 Издательство: Springer Рейтинг: Цена: 23508.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book covers the wide variety of enzymes in the field of enzyme biotechnology and details their industrial applications. It also includes new methods and state-of-the-art information on modern methods of enzyme discovery.
Автор: Antonio Baici Название: Kinetics of Enzyme-Modifier Interactions ISBN: 3709114012 ISBN-13(EAN): 9783709114018 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.
Описание: This book addresses issues arising from discharge of effluents from sugar industry on to surrounding land or into a water body such as physicochemical properties of soil, changes in the micro flora, quantification of soil enzyme activities as influenced by effluents. Disposal of effluents without neutralization has become general practice. These effluents are chemically heterogeneous, contain organic and inorganic pollutants including, sugar baggage, molasses, carbonates, bicarbonates. The impact of sugar industry effluents on microbial activities in terrestrial ecosystem is scanty. There is also significant interest in the study of soil enzymes because such effect reflects the potential capacity of a soil to perform certain biological transformation of soil fertility.
Автор: Judith Klinman; Sharon Hammes- Schiffer Название: Dynamics in Enzyme Catalysis ISBN: 3642389619 ISBN-13(EAN): 9783642389610 Издательство: Springer Рейтинг: Цена: 39130.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Cindy Schulenburg and Donald Hilvert, Protein Conformational Disorder and Enzyme Catalysis. Brooks III, Multiple Intermediates, Diverse Conformations, and Cooperative Conformational Changes Underlie the Catalytic Hydride Transfer Reaction of Dihydrofolate Reductase.
Автор: Jeffrey W. Kelly; Thomas O. Baldwin Название: Applications of Enzyme Biotechnology ISBN: 1475792379 ISBN-13(EAN): 9781475792379 Издательство: Springer Рейтинг: Цена: 26122.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Proceedings of the Texas A&M University IUCCP Ninth Annual Symposium held in College Station, Texas, March 18-21, 1991.
Описание: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with mo than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Описание: This volume of Methods in Enzymology is a companion to Volume 347 and addresses direct sensing of reactive oxygen species and related free radicals by thiol enzymes and proteins.
Автор: Colin Suckling Название: Enzyme Chemistry ISBN: 9401073171 ISBN-13(EAN): 9789401073172 Издательство: Springer Рейтинг: Цена: 20896.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: As the first edition of this book was going through the publication process, a revolution was taking place in the technologies available for the study of enzymes. The second edition of `Enzyme Chemistry, Impact and Applications` takes on board these new develop- ments whilst maintaining the overall aims and views of the first edition.
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