Adult and Pluripotent Stem Cells, J?rgen Hescheler; Erhard Hofer

Автор: Turksen
Название: Adult Stem Cells. 2004
ISBN: 1588291529 ISBN-13(EAN): 9781588291523
Издательство: Springer
Рейтинг:
Цена: 28734.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: 2-D Protein Gel Electrophoresis: An Overview, Jenny Fichmann and Reiner Westermeier. Solubilization of Proteins in 2-D Electrophoresis: An Outline, Thierry Rabilloud. Preparation of Escherichia coli Samples for 2-D Gel Analysis, Ruth A. VanBogelen and Frederick C. Neidhardt. Preparing 2-D Protein Extracts from Yeast, David M. Schieltz. 2-D Protein Extracts from Drosophila melanogaster, Christer Ericsson. Preparing 2-D Protein Extracts from Caenorhabditis elegans, Robert Zwilling. Eukaryotic Cell Labeling and Preparation for 2-D, Nick Bizios. Differential Detergent Fractionation of Eukaryotic Cells: Analysis by Two-Dimensional Gel Electrophoresis, Melinda L. Ramsby and Gregory S. Makowski. Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis, Joachim Klose. Preparation and Solubilization of Body Fluids for 2-D, Jean Charles Sanchez and Denis F. Hochstrasser. 2-D Electrophoresis of Plant Proteins, Akira Tsugita and Masaharu Kamo. Quantifying Protein in 2-D PAGE Solubilization Buffers, Louis S. Ramagli. Measuring the Radioactivity of 2-D Protein Extracts, Andrew J. Link and Nick Bizios. Advantages of Carrier Ampholyte IEF, Mary F. Lopez. 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF), Mary F. Lopez. Nonequilibrium pH Gel Electrophoresis (NEPHGE), Mary F. Lopez. High Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment, Marion Sarmiento. Large-Gel 2-D Electrophoresis, Joachim Klose. Advantages of Immobilized pH Gradients, Jenny Fichmann. Casting Immobilized pH Gradients (IPGs), Elisabetta Gianazza. Analytical IPG-Dalt, Angelika GГ¶rg and Walter Weiss. IPG-Dalt of Very Alkaline Proteins, Angelika GГ¶rg. Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D, Neil M. Matsui, Diana M. Smith-Beckerman, Jenny Fichmann, and Lois B. Epstein. In-Gel Sample Rehydration of Immobilized pH Gradient, Jean-Charles Sanchez, Denis Hochstrasser, and Thierry Rabilloud. High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis, Jean-Charles Sanchez and Denis F. Hochstrasser. Horizontal SDS-PAGE for IPG-Dalt, Angelika GГ¶rg and Walter Weiss. Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE, Bradley J. Walsh and Benjamin R. Herbert. Nonreducing 2-D Polyacrylamide Gel Electrophoresis, Hong Ji and Richard J. Simpson. 2-D Diagonal Gel Electrophoresis, Joan Goverman. 2-D Phosphopeptide Mapping, Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky, and Steven F. Dowdy. Internal Standards for 2-D, Andrew J. Link. Autoradiography of 2-D Gels, Andrew J. Link. Double-Label Analysis, Kelvin H. Lee and Michael G. Harrington. Silver Staining of 2-D Electrophoresis Gels, Thierry Rabilloud. Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining, Neil M. Matsui, Diana M. Smith-Beckerman, and Lois B. Epstein. Electroblotting of Proteins from 2-D Polyacrylamide Gels, Michael J. Dunn. Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels, Michael J. Dunn. Protein Detection Using Reversible Metal Chelate Stains, Wayne F. Patton, Mark J. Lim, and David Shepro. Gylcoprotein Detection of 2-D Separated Proteins, Nicolle H. Packer, Malcolm S. Ball, and Peter L. Devine. Image Acquisition in 2-D Electrophoresis, Wayne F. Patton, Mark J. Lim, and David Shepro. Computer Analysis of 2-D Images, Ron D. Appel and Denis F. Hochstrasser. 2-D Databases on the World Wide Web, Ron D. Appel, Amos Bairoch, and Denis F. Hochstrasser. Comparing 2-D Electrophoresis Gels Across Internet Databases, Peter F. Lemkin. Constructing a 2-D Database for the World Wide Web, Ron D. Appel, Christine Hoogland, Amos Bairoch, and Denis F. Hochstrasser. Absolute Quantitation of 2-D Protein Spots, Steven P. Gygi and Ruedi Aebersold. Generating a Bacterial Genome Inventory: Identifying 2-D Spots by Comigrating Products of the Genome on 2-D Gels, Ruth A. VanBogelen. Immunoaffinity Identification of 2-DE Separated Proteins, Barbara Magi, Luca Bini, Barbara Marzocchi, Sabrina Liberatori, Roberto Raggiaschi, and Vitaliano Pallini. 2-DE Spot Amino Acid Analysis with 9-Fluroenylmethyl Chloroformate, Marc R. Wilkins, Jun X. Yan, and Andrew A. Gooley. N-Terminal Amino Acid Sequencing of 2-DE Spots, Masaharu Kamo and Akira Tsugita. Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategies for Microsample Handling, Hediye Erdjument-Bromage, Mary Lui, Lynne Lacomis, and Paul Tempst. Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry, Rachel R. Ogorzalek Loo, Joseph A. Loo, and Philip C. Andrews. Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses, Paul L. Courchesne and Scott D. Patterson. Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Protein Identification and Analysis Tools in the ExPASy Server, Marc R. Wilkins, Elisabeth Gasteiger, Amos Bairoch, Jean-Charles Sanchez, Keith L. Williams, Ron D. Appel, and Denis F. Hochstrasser. Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry, John R. Yates III, Edwin Carmack, Lara Hays, Andrew J. Link, and Jimmy K. Eng. Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Index.

Автор: Xiaoyang Zhao
Название: Studies of pluripotency in embryonic stem cells and induced
ISBN: 9401788189 ISBN-13(EAN): 9789401788182
Издательство: Springer
Рейтинг:
Цена: 18167.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book covers methods to derive embryonic stem cells (ESCs) from several different species. It describes an efficient system to generate induced pluripotent stem cells (iPSCs) and reports on iPSC mice.

Автор: Kursad Turksen
Название: Adult Stem Cells
ISBN: 1461495687 ISBN-13(EAN): 9781461495680
Издательство: Springer
Рейтинг:
Цена: 23757.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Adult Stem Cells, second edition, takes a critical look at issues concerning the developmental or differentiation potential for a variety of tissue types and for specific adult stem cell types. Since the first edition appeared a decade ago, our understanding of adult stem cells, and more specifically tissue-specific adult stem cells, has advanced tremendously. And an increased interest in regenerative medicine and potential stem cell applications has driven a quest for better understanding of stem cell biology. In turn, this has spawned much activity on generation and utilization of more and better reagents to identify and isolate stem cells and stem cell-like subpopulations, and on assays elucidating their developmental or differentiation potential and functional integration with host tissues and organs. In this fully updated new edition, chapters cover topics ranging from signaling pathways maintaining stemness in hematopoietic cells to regeneration after injury and endocrine mechanisms underlying the stem cell theory of aging. Other chapters cover stem cells by organ or system including pituitary, cardiac, epithelial, teeth, lung, ovary, prostate, liver, and many more. Importantly, the authors of the chapters have not only summarized their successes, but have also summarized some of the difficulties that each particular field is still facing with respect to maximizing the utility of stem cells in clinical settings. Collectively, they impart both the excitement and challenges facing stem cell utilization for repair and regeneration making this book essential reading for those involved in stem cell research as well as those involved in clinical assays.

Автор: Kursad Turksen
Название: Adult and Embryonic Stem Cells
ISBN: 162703952X ISBN-13(EAN): 9781627039529
Издательство: Springer
Рейтинг:
Цена: 23508.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Collecting reviews first presented at the 2010 Stem Cell Symposia in Samsun, Turkey, this book covers embryonic and adult stem cell sources. Reviews the current state of knowledge as well as the challenges that underlie translational medicine applications.

Автор: Paolo Di Nardo; Sanjiv Dhingra; Dinender K. Singla
Название: Adult Stem Cells
ISBN: 1493967541 ISBN-13(EAN): 9781493967544
Издательство: Springer
Рейтинг:
Цена: 22359.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание:

Preface...
Table of Contents...
Contributing Authors...

1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells
Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta

2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells
Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra

3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice
Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran

4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer
Giulia Prosdocimo and Mauro Giacca

5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells
Ghazaleh Hajmousa and Martin C. Harmsen

6. Isolation and In Vitro Characterization of Epidermal Stem Cells
Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen

7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization
Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore

8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID
Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar

9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID
Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar

10. Isolation, Characterization, and Expansion of Cancer Stem Cells
Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia

11. Cardiac Progenitor Cell Isolation from Human Auricles
Paolo Di Nardo and Francesca Pagliari

12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle
Antonio Musarт and Silvia Carosio

13. Isolation of Stromal Stem Cells from Adipose Tissue
Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone

14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth
Mohammed Bakkar, Younan Liu , Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran

16. Measurement of Autophagy by Flow Cytometry
Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia

17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model
Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen

18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells
Federico Franchi and Martin Rodriguez-Porcel

19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells
Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra

20. Therapeutic Application of Adult Stem Cells in the Heart
Taylor A. Johnson and Dinender K. Singla

21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering
Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu

22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering
Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran

23. Optimal Enviro