Transcriptional Control of Lineage Differentiation in Immune Cells, Wilfried Ellmeier; Ichiro Taniuchi

Автор: Lugli
Название: T-Cell Differentiation
ISBN: 1493965468 ISBN-13(EAN): 9781493965465
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails, approaches to measure telomerase activity in terminally differentiated T cells, and approaches to define Treg cells at the phenotypic and functional level. The final part of the book is dedicated to the analysis of the differentiation and effector functions of innate T cells, namely the well-known ?/? T cells, and the recently identified CD8+ mucosal associated invariant T (MAIT) cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Differentiation: Methods and Protocols aims to provide protocols that are fundamental to monitor the T cell compartment at the level of single cells in pathological and immunotherapy conditions.

Автор: Bing Sun
Название: T Helper Cell Differentiation and Their Function
ISBN: 9401794863 ISBN-13(EAN): 9789401794862
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This book will focus on the differentiation and regulation of subsets of CD4+ T cells. It will also cover other aspects of research on these cells, which has made great advances in recent years, such as subsets’ plasticity and their role in healthy and disease conditions. The book provides researchers and graduate students with a cutting-edge and comprehensive overview of essential research on CD4+ T cells.