MRNA processing :, Shi, Yongsheng.

Автор: Thomas K?hler; Dirk La?ner; Anne-Katrin Rost; Barb
Название: Quantitation of mRNA by Polymerase Chain Reaction
ISBN: 3642797148 ISBN-13(EAN): 9783642797149
Издательство: Springer
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Описание: In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g.

Автор: Fabrice Lejeune
Название: Nonsense-Mediated mRNA Decay in Human Disease
ISBN: 0128044683 ISBN-13(EAN): 9780128044681
Издательство: Elsevier Science
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Описание: . Nonsense Mutation Correction in Human Diseases: An Approach for Targeted Medicine provides an introduction on genetic diseases, discusses the prevalence of nonsense mutations, the consequences of a nonsense mutation for the expression of the mutant gene, and the presentation of the nonsense-mediated mRNA decay (NMD). . It presents the mechanism of action and rationale associated with each strategy to correct nonsense mutations with the results of clinical trials to further support this basis. In addition, the book shows how it may be possible to combine several of these strategies to ultimately improve the efficiency of correction, also suggesting the future goals and objectives to improve treatment modalities in this evolving sphere of personalized medicine.

Автор: Klemens J. Hertel
Название: Spliceosomal Pre-mRNA Splicing
ISBN: 149396044X ISBN-13(EAN): 9781493960446
Издательство: Springer
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Цена: 16769.00 р.
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Описание:

Part I: Introductory Chapters

1. The Pre-mRNA Splicing Reaction

Somsakul Wongpalee and Shalini Sharma

2. Diversity and Evolution of Spliceosomal Systems

Scott William Roy and Manuel Irimia

3. Mechanisms of Spliceosomal Assembly

Ni-ting Chiou and Kristen W. Lynch

4. Alternative Pre-mRNA Splicing

Stacey D. Wagner and J. Andrew Berglund

5. Regulation of Alternative Pre-mRNA Splicing

Miguel B. Coelho and Christopher W.J. Smith

6. Introduction to Co-Transcriptional RNA Splicing

Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson

7. Chromatin and Splicing

Nazmul Haque and Shalini Oberdoerffer

Part II: Methods Chapters

8. Preparation of Splicing Competent Nuclear Extracts

Chiu-Ho T. Webb and Klemens J. Hertel

9. Preparation of Yeast Whole Cell Splicing Extract

Elizabeth A. Dunn and Stephen D. Rader

10. Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase

Martha R. Stark and Stephen D. Rader

11. In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract

Maliheh Movassat, William F. Mueller, and Klemens J. Hertel

12. Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract

William F. Mueller and Klemens J. Hertel

13. In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation

Eric G. Folco and Robin Reed

14. Isolation and Accumulation of Spliceosomal Assembly Intermediates

Janine O. Ilagan and Melissa S. Jurica

15. Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function

Martha R. Stark and Stephen D. Rader

16. Expression and Purification of Splicing Proteins from Mammalian Cells

Eric Allemand and Michelle L. Hastings

17. Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis

Eric G. Anderson and Aaron A. Hoskins

18. Cell-Based Splicing of Minigenes

Sarah A. Smith and Kristen W. Lynch

19. Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA

Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal

20. Antisense Methods to Modulate Pre-mRNA Splicing

Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh

21. Using Yeast Genetics to Study Splicing Mechanisms

Munshi Azad Hossain and Tracy L. Johnson

22. Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR

Ryan Percifield, Daniel Murphy, and Peter Stoilov

23. Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing

Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer

24. Computational Approaches to Mine Publicly Available Databases

Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund

25. Approaches to Link RNA Secondary Structures with Splicing Regulation

Mireya Plass and Eduardo Eyras

26. Methods to Study Splicing from High-Throughput RNA Sequencing Data

Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras

27. Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq

Chengguo Yao, Lingjie Weng, and Yongsheng Shi

28. Predicting Alternative Splicing

Yoseph Barash and Jorge Vaquero Garcia

Автор: Robert E. Rhoads
Название: Synthetic mRNA
ISBN: 1493936239 ISBN-13(EAN): 9781493936236
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy.

Автор: Klemens J. Hertel
Название: Spliceosomal Pre-mRNA Splicing
ISBN: 1627039791 ISBN-13(EAN): 9781627039796
Издательство: Springer
Рейтинг:
Цена: 16769.00 р.
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Описание:

Part I: Introductory Chapters

1. The Pre-mRNA Splicing Reaction

Somsakul Wongpalee and Shalini Sharma

2. Diversity and Evolution of Spliceosomal Systems

Scott William Roy and Manuel Irimia

3. Mechanisms of Spliceosomal Assembly

Ni-ting Chiou and Kristen W. Lynch

4. Alternative Pre-mRNA Splicing

Stacey D. Wagner and J. Andrew Berglund

5. Regulation of Alternative Pre-mRNA Splicing

Miguel B. Coelho and Christopher W.J. Smith

6. Introduction to Co-Transcriptional RNA Splicing

Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson

7. Chromatin and Splicing

Nazmul Haque and Shalini Oberdoerffer

Part II: Methods Chapters

8. Preparation of Splicing Competent Nuclear Extracts

Chiu-Ho T. Webb and Klemens J. Hertel

9. Preparation of Yeast Whole Cell Splicing Extract

Elizabeth A. Dunn and Stephen D. Rader

10. Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase

Martha R. Stark and Stephen D. Rader

11. In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract

Maliheh Movassat, William F. Mueller, and Klemens J. Hertel

12. Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract

William F. Mueller and Klemens J. Hertel

13. In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation

Eric G. Folco and Robin Reed

14. Isolation and Accumulation of Spliceosomal Assembly Intermediates

Janine O. Ilagan and Melissa S. Jurica

15. Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function

Martha R. Stark and Stephen D. Rader

16. Expression and Purification of Splicing Proteins from Mammalian Cells

Eric Allemand and Michelle L. Hastings

17. Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis

Eric G. Anderson and Aaron A. Hoskins

18. Cell-Based Splicing of Minigenes

Sarah A. Smith and Kristen W. Lynch

19. Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA

Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal

20. Antisense Methods to Modulate Pre-mRNA Splicing

Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh

21. Using Yeast Genetics to Study Splicing Mechanisms

Munshi Azad Hossain and Tracy L. Johnson

22. Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR

Ryan Percifield, Daniel Murphy, and Peter Stoilov

23. Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing

Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer

24. Computational Approaches to Mine Publicly Available Databases

Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund

25. Approaches to Link RNA Secondary Structures with Splicing Regulation

Mireya Plass and Eduardo Eyras

26. Methods to Study Splicing from High-Throughput RNA Sequencing Data

Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras

27. Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq

Chengguo Yao, Lingjie Weng, and Yongsheng Shi

28. Predicting Alternative Splicing

Yoseph Barash and Jorge Vaquero Garcia

Автор: Friedrich Marks, Ursula Klingmuller, Karin Muller- Decker
Название: Cellular Signal Processing: An Introduction to the Molecular Mechanisms of Signal Transduction
ISBN: 0815345348 ISBN-13(EAN): 9780815345343
Издательство: Taylor&Francis
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Цена: 11023.00 р.
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Описание:

Cellular Signal Processing offers a unifying view of cell signaling based on the concept that protein interactions act as sophisticated data processing networks that govern intracellular and extracellular communication. It is intended for use in signal transduction courses for undergraduate and graduate students working in biology, biochemistry, bioinformatics, and pharmacology, as well as medical students. The text is organized by three key topics central to signal transduction: the protein network, its energy supply, and its evolution. It covers all important aspects of cell signaling, ranging from prokaryotic signal transduction to neuronal signaling, and also highlights the clinical aspects of cell signaling in health and disease. This new edition includes expanded coverage of prokaryotes, as well as content on new developments in systems biology, epigenetics, redox signaling, and small, non-coding RNA signaling.

Автор: Edgardo R. Donati; Wolfgang Sand
Название: Microbial Processing of Metal Sulfides
ISBN: 9048174023 ISBN-13(EAN): 9789048174027
Издательство: Springer
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Цена: 26120.00 р.
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Описание: The application of microbiological methods to the extraction of metals from minerals is supported by several bioleaching and biooxidation processes operating in different sites over the world.

Автор: Michael Lones; Andy Tyrrell; Stephen Smith; Gary F
Название: Information Processing in Cells and Tissues
ISBN: 3319231073 ISBN-13(EAN): 9783319231075
Издательство: Springer
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Цена: 6708.00 р.
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Описание: This book constitutes the proceedings of the 10th International Conference on Information Processing in Cells and Tissues, IPCAT 2015, held in San Diego, CA, USA, in September 2015. They were organized in topical sections named: biochemical information processing; biochemical regulatory networks; and neural modelling and neural networks.

Автор: Guillaume S. Masson; Uwe J. Ilg
Название: Dynamics of Visual Motion Processing
ISBN: 1489989951 ISBN-13(EAN): 9781489989956
Издательство: Springer
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Цена: 27950.00 р.
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Описание: Motion processing is an essential piece of the complex brain machinery that allows us to reconstruct the 3D layout of objects in the environment, to break camouflage, to perform scene segmentation, to estimate the ego movement, and to control our action. Although motion perception and its neural basis have been a topic of intensive research and modeling the last two decades, recent experimental evidences have stressed the dynamical aspects of motion integration and segmentation. This book presents the most recent approaches that have changed our view of biological motion processing. These new experimental evidences call for new models emphasizing the collective dynamics of large population of neurons rather than the properties of separate individual filters. Chapters will stress how the dynamics of motion processing can be used as a general approach to understand the brain dynamics itself.

Автор: Yeo
Название: RNA Processing
ISBN: 3319290711 ISBN-13(EAN): 9783319290713
Издательство: Springer
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Цена: 22203.00 р.
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Описание:

Автор: Amit Kumar; Fahimuddin Shaik; B Abdul Rahim; D.Sra
Название: Signal and Image Processing in Medical Applications
ISBN: 981100689X ISBN-13(EAN): 9789811006890
Издательство: Springer
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Цена: 9141.00 р.
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Описание: This book highlights recentfindings on and analysesconducted on signalsand images in the area of medicine. The bookexplains how signal and image processing methods can be used to detect andforecast abnormalities in an easy-to-followmanner, offering a valuable resource for researchers, engineers,physicians and bioinformatics researchers alike.

Автор: Nikolaos E. Labrou
Название: Protein Downstream Processing
ISBN: 1627039767 ISBN-13(EAN): 9781627039765
Издательство: Springer
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Цена: 30745.00 р.
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Описание:

Part I. Screening and Design Purification Strategies

1. Protein Purification: An Overview

Nikolaos Labrou

2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE

Ignacio Amadeo, Laura Mauro, Eduardo Ortн, and Guillermina Forno

3. High Throughput Process Development: I. Process Chromatography

A. S. Rathore and R. Bhambure

4. High Throughput Process Development: II. Membrane Chromatography

A. S. Rathore and S. Muthukumar

5. Media Selection in Ion Exchange Chromatography in a Single Microplate

Charlotte Cabanne and Xavier Santarelli

6. High Throughput Screening of Dye-Ligands for Chromatography

Sunil Kumar and Narayan S. Punekar

7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips

Sunil Chhatre and Daniel G. Bracewell

Part II. Low-Resolution Protein Purification Methods

8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline

Zhengrong GU

9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants

Oscar Aguilar and Marco Rito-Palomares

10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins

A. S. Rathore and R. Bhambure

11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)

Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta

12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm

Ario de Marco

13. Immunoglobulin Purification by Caprylic Acid

Victor Morais, Patricia Berasain, and Hugo Massaldi

Part III: High-Resolution Protein Purification Methods

14. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

Ana Sofia Pina, Нris L. Batalha, and Ana Cecнlia A. Roque

15. Expression and Purification of Large Active GST Fusion Enzymes

Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa

16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins

Sharon Williams, Phil Morton, and Dev Baines

17. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery

My Hedhammar, Johan Nilvebrant, and Sophia Hober

18. An Orthogonal Fusion Tag for Efficient Protein Purification

Johan Nilvebrant, Mikael Еstrand, and Sophia Hober

19. Phage Display of Engineered Binding Proteins

Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servй W.M. Kengen, and John van der Oost

20. Biomimetic Affinity Ligands for Protein Purification

Isabel T. Sousa and M. Вngela Taipa

21. Synthesis and Application of Dye-Ligand Affinity Adsorbents

Evangelia Chronopoulou and Nikolaos E. Labrou

22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

S. A. Camperi, M. C. Martнnez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone

23. Affinity Chromatography of Proteins on Monolithic Columns

E.G. Vlakh, G.A. Platonova, and T.B. Tennikova

24. Sample Displacement Batch Chromatography of Proteins

Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlьter

Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization

25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches

Catalin E. Doneanu, Weibin Chen, an