Proteomic Methods in Neuropsychiatric Research, Paul C. Guest

Автор: Nedelkov Dobrin, Nelson Randall W.
Название: New and Emerging Proteomic Techniques
ISBN: 1588295192 ISBN-13(EAN): 9781588295194
Издательство: Springer
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Цена: 20263.00 р.
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Описание: Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biologyв„ў series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Автор: Pawel Ciborowski
Название: Proteomic Profiling and Analytical Chemistry
ISBN: 0444636889 ISBN-13(EAN): 9780444636881
Издательство: Elsevier Science
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"Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition" helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.

The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measureIncludes practical examples illustrating the importance of problems in quantitation and validation of biomarkersHelps in designing and executing proteomic experiments with sound analytics

Автор: P. Michael Conn
Название: Handbook of Proteomic Methods
ISBN: 1617375047 ISBN-13(EAN): 9781617375040
Издательство: Springer
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Цена: 26120.00 р.
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Описание: Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms.