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DNA Methylation Protocols, J?rg Tost


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Автор: J?rg Tost
Название:  DNA Methylation Protocols
ISBN: 9781493974795
Издательство: Springer
Классификация:

ISBN-10: 1493974793
Обложка/Формат: Hardcover
Страницы: 597
Вес: 1.45 кг.
Дата издания: 10.12.2017
Серия: Methods in Molecular Biology
Язык: English
Издание: 3rd ed. 2019
Иллюстрации: 67 illustrations, color; 35 illustrations, black and white; xviii, 704 p. 102 illus., 67 illus. in color.
Размер: 254 x 178 x 38
Читательская аудитория: Postgraduate, research & scholarly
Основная тема: Biomedicine
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание:
This third edition volume expands on the previous editions by providing a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials
and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.


Дополнительное описание:
A Summary of the Biological Processes, Disease-Associated Changes and Clinical Applications of DNA Methylation.- Considerations for Design and Analysis of DNA Methylation Studies.- Quantification of Global DNA Methylation Levels by Mass Spectrometry.



In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
Наличие на складе: Есть (4 шт.)
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Forensic DNA Typing Protocols

Автор: Goodwin
Название: Forensic DNA Typing Protocols
ISBN: 149393595X ISBN-13(EAN): 9781493935956
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Thisvolume presents a series of protocols and methods, some of which are not widelyused by researchers/practitioners, and will aid in the execution of differentlaboratory techniques. Forensic DNA Typing Protocols, Second Edition isarranged into a series of related chapters. Chapter 1-3 examines two differentaspects of RNA analysis for body fluid identification. Chapters 4-7 focuses onthe storage of biological materials and the extraction of DNA from hardtissues. Chapters 8-10 present methods for monitoring the quality of DNAextracts, and steps to aid in the purification of DNA. Chapters 11-16 talkabout methods on non-standard markers, such as INDELs, Y chromosome STRs, andmitochondrial DNA. Detailed procedures and data analysis for phenotypes andancestry are explored in Chapter 17-19. The last chapter (20) looks at theapplication of DNA typing to the identification of non-human material tospecies level. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics,lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Practical and thorough, Forensic DNA Typing Protocols, Second Edition, isa valuable resource for forensic specialists, researchers, and anyoneinterested in the field of forensic science.

DNA Methylation: Development, Genetic Disease and Cancer

Автор: Walter Doerfler; Petra B?hm
Название: DNA Methylation: Development, Genetic Disease and Cancer
ISBN: 3642068308 ISBN-13(EAN): 9783642068300
Издательство: Springer
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Цена: 26120.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: It has become apparent that the genomes of many organisms are characterized by unique patterns of DNA methylation which can differ from genome segment to genome segment and cell type to cell type.

RNA Methylation

Автор: Alexandra Lusser
Название: RNA Methylation
ISBN: 149396805X ISBN-13(EAN): 9781493968053
Издательство: Springer
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Цена: 20962.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation.

DNA Electrophoresis Protocols for Forensic Genetics

Автор: Antonio Alonso
Название: DNA Electrophoresis Protocols for Forensic Genetics
ISBN: 1493962191 ISBN-13(EAN): 9781493962198
Издательство: Springer
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Цена: 20263.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume in the Methods in Molecular Biology (TM) series covers methods in forensic DNA profiling, including protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2.

DNA Repair Protocols

Автор: Lotte Bjergb?k
Название: DNA Repair Protocols
ISBN: 1493959549 ISBN-13(EAN): 9781493959549
Издательство: Springer
Рейтинг:
Цена: 21661.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: In its 3rd edition, this Methods in Molecular Biology (TM) book covers the eukaryotic response to genomic insult including advanced protocols and standard techniques in the field of DNA repair. Offers expert guidance for DNA repair, recombination, and replication.

DNA Methylation

Автор: J. Jost; H. Saluz
Название: DNA Methylation
ISBN: 3034899157 ISBN-13(EAN): 9783034899154
Издательство: Springer
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Цена: 15672.00 р.
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Описание: The explosive increase in the number of publications dealing with DNA methylation attests to its importance and makes it impossible to write a comprehensive coverage of the literature within the scope of a general review. Since the publication of the 3 most recent books dealing with the subject (DNA methylation by Razin A.


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