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Phospho-Proteomics, Louise von Stechow


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Автор: Louise von Stechow
Название:  Phospho-Proteomics
ISBN: 9781493950010
Издательство: Springer
Классификация:

ISBN-10: 1493950010
Обложка/Формат: Paperback
Страницы: 364
Вес: 0.72 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Размер: 254 x 178 x 20
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание:

Part I: Techniques for Tagging Phospho-Moieties

1. Thiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins

Kaelyn E. Wilke and Erin E. Carlson

2. Phosphopeptide Detection with Biotin-Labeled Phos-tag

Emiko Kinoshita-Kikuta, Eiji Kinoshita and Tohru Koike

3. Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports

Heinz Nika, Ruth Hogue Angeletti, and David H. Hawke

Part II: Labeling Techniques for Quantitative Phosphoproteomics

4. Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics

Lei Cheng, Trairak Pisitkun, Mark A. Knepper, and Jason D. Hoffert

5. Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles

Daniel A. Rothenberg, Elizabeth A. Gordon, Forest M. White, and Sebastian Lourido

6. Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM)

Ryohei Narumi and Takeshi Tomonaga

Part III: Phosphopeptide Enrichment Strategies

7. Enrichment Strategies in Phosphoproteomics

Alexander Leitner

8. Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography

Tine E. Thingholm and Martin R. Larsen

9. The use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides

Tine E. Thingholm and Martin R. Larsen

10. Sequential Elution from IMAC (SIMAC) - An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated Peptides

Tine E. Thingholm and Martin R. Larsen

11. Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation

Kasper Engholm-Keller and Martin R. Larsen

12. Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage

Tanveer S Batth, Jesper V Olsen

13. Phosphopeptide Enrichment Using Various Magnetic Nanocomposites - An Overview

Нris L. Batalha and Ana Cecнlia A. Roque

Part IV: Phosphoprotein Analysis by Mass Spectrometry

14. Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics

Chao Han and Pingfang Yang

15. Variable Digestion Strategies for Phosphoproteomics Analysis

Humberto Gonczarowska-Jorge, Margherita DellAica, Clarissa Dickhut, and Renй P. Zahedi

16. On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins

Hongyan Zhao, Andrew J. Creese, and Helen J. Cooper

17. Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High Sensitivity

Rosa R. Jersie-Christensen, Abida Sultan, and Jesper V. Olsen

Part V: High-throughput Studies of the Phosphoproteome

18. Identification of Direct Kinase Substrates via Kinase Assay Linked Phosphoproteomics

Liang Xue, Justine V. Arrington, and W. Andy Tao

19. Phosphoprotein Detection by High-Throughput Flow Cytometry

Johannes Landskron and Kjetil Taskйn

Part VI: Bioinformatics Resources for Phosphoproteomics Analyses

20. Resources for Assignment of Phosphorylation Sites on Peptides and Proteins

Vaishnavi Ravikumar, Boris Macek, and Ivan Mijakovic

21. From Phosphosites to Kinases

Stephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen, and Lars J. Jensen

22. Search Databases and Statistics - Pitfalls



Mass Spectrometry Data Analysis in Proteomics

Автор: Matthiesen Rune
Название: Mass Spectrometry Data Analysis in Proteomics
ISBN: 158829563X ISBN-13(EAN): 9781588295637
Издательство: Springer
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Цена: 23757.00 р.
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Описание: Aims to support researchers in deciding which programs to use in various tasks related to analysis of mass spectrometry data in proteomics. This work covers chapters that give a description of the theoretical background of each topic followed by descriptions of programs and the parameters best suited for different cases.

Genomics and Proteomics for Clinical Discovery and Development

Автор: Gy?rgy Marko-Varga
Название: Genomics and Proteomics for Clinical Discovery and Development
ISBN: 9401792011 ISBN-13(EAN): 9789401792011
Издательство: Springer
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Цена: 19564.00 р.
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Описание: The book is intended to be a resource for students as well as scientists in education and for the general public to learn about proteomics and genomics. Chromosomes form the basis for our genetic heritage and are the code for protein synthesis. The Human Genome Map came out in 2002, and the Proteome Sequence Map is under currently being created by a global consortia initiative. Proteome and genome building blocks already form the basis of scientific research areas as well as large parts of the pharmaceutical and biomedical industry. The book initiative will provide the background to and our current understanding of these gene and protein areas, as well as describe how cutting-edge science is using these resources to develop new medicines and new diagnostics for patient care and treatment. The book will be useful for undergraduate students as well as university students and researchers who need a good understanding of genomics and proteomics within the clinical field. The book will also be targeted at a broad public as well as readers not specialized within this field. Dr. Marko-Varga is the head of the Head of Div. Clinical Protein Science & Imaging at the Biomedical Center, Dept. of Measurement Technology and Industrial Electrical Engineering, Lund University, and Professor at the 1st Department of Surgery, Tokyo Medical University, Tokyo, Japan.

Phospho-Proteomics

Автор: Marjo de Graauw
Название: Phospho-Proteomics
ISBN: 1617379174 ISBN-13(EAN): 9781617379178
Издательство: Springer
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Цена: 20263.00 р.
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Описание: For this volume, top researchers have contributed both established protocols and some of the newest strategies to identify and evaluate protein phosphorylation on Tyr, Ser, and Thr residues, including topics such as 2-dimensional gel electrophoresis.

Phospho-proteomics

Название: Phospho-proteomics
ISBN: 1493930486 ISBN-13(EAN): 9781493930487
Издательство: Springer
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Цена: 20962.00 р.
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Chemical Proteomics

Автор: Gerard Drewes; Marcus Bantscheff
Название: Chemical Proteomics
ISBN: 1493958003 ISBN-13(EAN): 9781493958009
Издательство: Springer
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Цена: 16070.00 р.
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Описание: The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function. In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.

Proteomics in Human Reproduction

Автор: Agarwal
Название: Proteomics in Human Reproduction
ISBN: 3319484168 ISBN-13(EAN): 9783319484167
Издательство: Springer
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Цена: 7685.00 р.
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Описание: This Brief explores the use of proteomics as a tool for biomarker discovery in human reproduction and summarizes current findings and trends of proteomic studies in both male and female infertility. This simplifies this important but complex topic and equips the novice reader with sufficient background information on the use of proteomics in human reproduction. The up-to-date scenario on proteomic investigations will also appeal to researchers and post graduate students looking to keep abreast with the latest developments in reproductive research. This review summarizes current findings of contemporary proteomic studies on infertility in both males and females with various reproductive pathologies, and its use in predicting the outcome of assisted reproduction. In human reproduction, the search for biomarkers via proteomics is a fast-evolving approach that involves the analysis of proteins in the reproductive tissues and fluids, such as the male gametes, seminal plasma, ovarian and endometrial tissue, and follicular and uterine fluid. By comparing the protein profile of a healthy, fertile individual against that of an infertile individual, the differentially expressed proteins may give an indication to certain proteins that could serve as useful biomarkers that are related to infertility. As proteomic studies continue to unravel the dynamic proteome behind various infertility conditions, there is potential for the discovery of prognostic markers that could ultimately help in both natural and assisted human reproduction.

Structural Proteomics

Автор: Bostjan Kobe; Mitchell Guss; Thomas Huber
Название: Structural Proteomics
ISBN: 1617377899 ISBN-13(EAN): 9781617377891
Издательство: Springer
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Цена: 23058.00 р.
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Описание: This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives.

Membrane Proteomics

Автор: Matthew J. Peirce; Robin Wait
Название: Membrane Proteomics
ISBN: 1617378895 ISBN-13(EAN): 9781617378898
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This book collects together in one volume the diverse practical approaches to analyzing membrane proteins. This is an ideal guide for investigators wishing to apply state-of-the-art membrane proteomic methodologies in their own research programs.

Heart Proteomics

Автор: Fernando Vivanco
Название: Heart Proteomics
ISBN: 1493960008 ISBN-13(EAN): 9781493960002
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This Methods in Molecular Biology (TM) book offers protocols and updated methods with a particular focus on MS-based methods of protein and peptide quantification and the analysis of posttranslational modifications as well as descriptions of system biology methods.

Mass Spectrometry Data Analysis in Proteomics

Автор: Rune Matthiesen
Название: Mass Spectrometry Data Analysis in Proteomics
ISBN: 1617376442 ISBN-13(EAN): 9781617376443
Издательство: Springer
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Цена: 25853.00 р.
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Описание: This is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters.

Organelle Proteomics

Автор: Delphine Pflieger; Jean Rossier
Название: Organelle Proteomics
ISBN: 1617377724 ISBN-13(EAN): 9781617377723
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This is the first book to examine organelle proteomics in depth.It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis.


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