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Signal Transduction Immunohistochemistry, Alexander E. Kalyuzhny


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Автор: Alexander E. Kalyuzhny
Название:  Signal Transduction Immunohistochemistry
ISBN: 9781493958047
Издательство: Springer
Классификация:

ISBN-10: 1493958046
Обложка/Формат: Paperback
Страницы: 302
Вес: 0.60 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Размер: 254 x 178 x 17
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: This book presents protocols that address the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Coverage includes protocols in digital imaging and image analysis of stained cells and techniques used in cancer and stem cell research.


Immunohistochemistry

Автор: Buchwalow, Igor B. Bocker, Werner
Название: Immunohistochemistry
ISBN: 3642046088 ISBN-13(EAN): 9783642046087
Издательство: Springer
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Цена: 21522.00 р. 30745.00 -30%
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Описание: This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.

Cellular Signal Processing: An Introduction to the Molecular Mechanisms of Signal Transduction

Автор: Friedrich Marks, Ursula Klingmuller, Karin Muller- Decker
Название: Cellular Signal Processing: An Introduction to the Molecular Mechanisms of Signal Transduction
ISBN: 0815345348 ISBN-13(EAN): 9780815345343
Издательство: Taylor&Francis
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Цена: 11023.00 р.
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Описание:

Cellular Signal Processing offers a unifying view of cell signaling based on the concept that protein interactions act as sophisticated data processing networks that govern intracellular and extracellular communication. It is intended for use in signal transduction courses for undergraduate and graduate students working in biology, biochemistry, bioinformatics, and pharmacology, as well as medical students. The text is organized by three key topics central to signal transduction: the protein network, its energy supply, and its evolution. It covers all important aspects of cell signaling, ranging from prokaryotic signal transduction to neuronal signaling, and also highlights the clinical aspects of cell signaling in health and disease. This new edition includes expanded coverage of prokaryotes, as well as content on new developments in systems biology, epigenetics, redox signaling, and small, non-coding RNA signaling.

Signal Transduction Immunohistochemistry

Автор: Alexander E. Kalyuzhny
Название: Signal Transduction Immunohistochemistry
ISBN: 1493967576 ISBN-13(EAN): 9781493967575
Издательство: Springer
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Цена: 19564.00 р.
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Описание:

Preface...
Table of Contents...
Contributing Authors...

1. Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications
Kathy Brumbaugh, Wen-Chie Liao, J. P. Houchins, Jeff Cooper, and Steve Stoesz

2. Antibodies That Work Again and Again and Again
Kameron Simpson and Michael Browning

3. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods
Biji T. Kurien and R. Hal Scofield

4. Acquisition and Post-Processing of Immunohistochemical Images
Jerry Sedgewick

5. Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction
Jessica Kalra and Jennifer Baker

6. EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
Christianne Bandeira-Melo, Ligia Almeida Paiva, Natбlia R. T. Amorim, Peter F. Weller, and Patricia T. Bozza

7. Preservation of Specific Protein Signaling States using Heat Based Stabilizor System
Mats Borйn

8. Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species
Jodi Hagen, David Schwartz, and Alexander E. Kalyuzhny

9. Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages
Charles A. Warwick and Yuriy M. Usachev

10. Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines and Surface Markers
Chris Goetz, Li-Jen Peng, Birte Aggeler, and Jody Bonnevier

11. A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy
Aishwarya Sathyanarayan

12. Apoptotic Bodies: Selective Detection in Extracellular Vesicles
Paul Hauser, Sha Wang, and Vladimir V. Didenko

13. Activation and Differentiation of Mesenchymal Stem Cells
Pravin J. Mishra and Debabrata Banerjee

14. High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP
Michael Grahek, Ana Ptak, and Alexander E. Kalyuzhny

15. Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry
David Schwartz, Michael Grahek, Yingwei He, Wei Wang, Jennifer Nguyen, and Alexander E. Kalyuzhny

16. Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D
Candace C. Minchew and Vladimir V. Didenko

17. Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment
Jessica Kalra and Jennifer Baker

18. Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors
Jacob Ricca, Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan, Matthew Brendel, Dmitriy Zamarin, Jedd D. Wolchok, and Katia Manova-Todorova

19. Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt
Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan, Matthew Brendel, and Katia Manova-Todorova

20. Automated High Content Screening of g--H2AX Expression in HeLa Cells
Nate Hopp, Jodi Hagen, Birte Aggeler, and Alexander E. Kalyuzhny

Immunohistochemistry: Basics and Methods

Автор: Igor B. Buchwalow; Werner B?cker
Название: Immunohistochemistry: Basics and Methods
ISBN: 364242502X ISBN-13(EAN): 9783642425028
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.

Immunohistochemistry  Essential Elements and Beyond

Автор: Kalyuzhny, Alexander E.
Название: Immunohistochemistry Essential Elements and Beyond
ISBN: 3319308912 ISBN-13(EAN): 9783319308913
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Short Historic Overview.- Primary Antibodies.- Choosing Commercial Suppliers of Primary Antibodies.- Detection and Visualization of Antigens.- Ancillary Reagents for IHC.- Preparing Tissues for IHC Staining.- Staining Protocols.- Microscopy Examination and Collection of Digital Images and Their Presentation.- Concluding Remarks.

Signal Transduction Immunohistochemistry

Автор: Kalyuzhny
Название: Signal Transduction Immunohistochemistry
ISBN: 1617790230 ISBN-13(EAN): 9781617790232
Издательство: Springer
Рейтинг:
Цена: 20263.00 р.
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Описание: Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.  In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC).  Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.

Technical aspects of toxicological immunohistochemistry

Название: Technical aspects of toxicological immunohistochemistry
ISBN: 1493915150 ISBN-13(EAN): 9781493915156
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This authoritative volume examines immunohistochemical methods aimed at investigating the toxicologic pathology of rodent, non-human primate and aquatic animal tissues.

Signal Transduction and the Gasotransmitters / NO, CO, and H2S in Biology and Medicine

Автор: Wang Rui
Название: Signal Transduction and the Gasotransmitters / NO, CO, and H2S in Biology and Medicine
ISBN: 1588293491 ISBN-13(EAN): 9781588293497
Издательство: Springer
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Цена: 23757.00 р.
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Описание: Distinguished researchers and clinicians review the biological and biomedical aspects of gasotransmitters, emphasizing signaling transduction mechanisms in general, and ion channel regulation in particular. The authors discuss the endogeneous metabolism and regulation of gasotransmitters, their toxicological profiles and biological actions, and their interactions in terms of their production and effects. The physiological roles of NO, CO, and H2S in the regulation of the cardiovascular, neuronal, and gastrointestinal systems, as well as of cell metabolism, are also reviewed, along with the interaction of the gastrotransmitters with KATP,KCa voltage-gated Ca2+, voltage-gated Na+, and cyclic nucleotide-gated ion channels.


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