Julie A. Douthwaite; Ronald H. Jackson Ribosome Display and Related Technologies 9781493958726

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Автор: Julie A. Douthwaite; Ronald H. Jackson
Название: Ribosome Display and Related Technologies
ISBN: 9781493958726
Издательство: Springer
Классификация:

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ISBN-10: 1493958720
Обложка/Формат: Paperback
Страницы: 424
Вес: 0.83 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 10 tables, black and white; xiii, 424 p.
Размер: 254 x 178 x 23
Читательская аудитория: General (us: trade)
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread. Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols. The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.
Дополнительное описание: Ribosome Display: A Perspective.- Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts.- Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate.- Stabilized Ribosome Display for In Vitro Selection.- Eukaryotic

Ribosome Display and Related Technologies, Julie A. Douthwaite; Ronald H. Jackson