Ribosomes, Alexander Spirin

Автор: Marina V. Rodnina; Wolfgang Wintermeyer; Rachel Gr
Название: Ribosomes Structure, Function, and Dynamics
ISBN: 3709119286 ISBN-13(EAN): 9783709119280
Издательство: Springer
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Описание: This book collects over 40 articles based on the talks presented at the 2010 Ribosome Meeting, held in Orvieto, Italy, covering all facets of the structure and function of the ribosome. The up-to-date reviews will serve as a source of essential information.

Автор: Alexander S. Spirin
Название: Ribosomes
ISBN: 0306461455 ISBN-13(EAN): 9780306461453
Издательство: Springer
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Описание: This text is for advanced undergraduates and new graduate students and covers the structure, function, and biosynthesis of ribosomes. Ribosomes are important in protein synthesis, which is currently a hot topic in many different areas of research.

Автор: Boyd Hardesty; Gisela Kramer
Название: Structure, Function, and Genetics of Ribosomes
ISBN: 1461293464 ISBN-13(EAN): 9781461293460
Издательство: Springer
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Описание: In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was transmitted from one organism to another, or the extent to which the chemical basis of life is unified.

Автор: A.A. Hadjiolov
Название: The Nucleolus and Ribosome Biogenesis
ISBN: 3709187443 ISBN-13(EAN): 9783709187449
Издательство: Springer
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Автор: Julie A. Douthwaite; Ronald H. Jackson
Название: Ribosome Display and Related Technologies
ISBN: 1493958720 ISBN-13(EAN): 9781493958726
Издательство: Springer
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Описание: Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread. Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols. The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.

Автор: H. Bielka; H. Bielka; U. A. Bommer; H. Welfle; F.
Название: The Eukaryotic Ribosome
ISBN: 364268274X ISBN-13(EAN): 9783642682742
Издательство: Springer
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Описание: Contents References 11 II. A short historical survey 13 References 16 III. Ribosomes within the cell 20 1. Proportion of ribosomes in tissues 21 2. Free and membrane-bound ribo- mes 22 3. Ribosomes in nucleoli 26 4. Structure of polysomes in the cell 26 5. Ribosomal crystals 33 6. References 33 IV. Some general properties of ribosomes 41 1. Physical characteristics 41 2. Chemical characteristics 43 3. References 44 V. Morphology of ribosomes and poly- somes 47 1. Electron microscopy 47 a) The small ribosomal subunit 48 b) The large ribosomal subunit 48 c) The monomeric ribosome 52 d) Three-dimensional models 54 e) Polysomes 55 2. Small-angle X-ray scattering 55 a) The monomeric ribosome 56 b) Polysomes 57 3. References 57 VI. Chemical components 61 1. Ribosomal proteins 61 a) Electrophoretic separation and num- ber 61 b) Preparation of single ribosomal pro- teins 67 c) Molecular weights 68 d) Amino acid composition and amino acid sequences 71 e) Stoichiometry 71 f) Posttranslational modifications 72 g) Comparison of ribosomal proteins of different tissues 73 h) Species specificities and evolution 74 i) Ribosomal proteins of mitochondria and chloroplasts 75 k) Ribosomal mutants with altered pro- teins 75 5 3. Formation of preribosomal 2. Phosphorylation of ribosomal prote- particles 139 ins 76 4. References 141 a) In vitro phosphorylation and de- phosphorylation 76 VIII. Dissociation - reassociation processes b) In vivo phosphorylation 77 of ribosomal particles 151 c) Phosphorylation of ribosomal pro- tein S 6 78 1.