High-Throughput Next Generation Sequencing, Young Min Kwon; Steven C. Ricke
Автор: Gordan Lauc Название: High-Throughput Glycomics and Glycoproteomics ISBN: 1493964917 ISBN-13(EAN): 9781493964918 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.
Автор: Lee-Jun C. Wong Название: Next Generation Sequencing ISBN: 1461470005 ISBN-13(EAN): 9781461470007 Издательство: Springer Рейтинг: Цена: 30745.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book examines next generation sequencing applications. It details current technologies, various gene enrichment methods and massively parallel sequencing platforms, potential limitations, and the application of these technologies.
Автор: Wei Wu; Hani Choudhry Название: Next Generation Sequencing in Cancer Research ISBN: 1461476445 ISBN-13(EAN): 9781461476443 Издательство: Springer Рейтинг: Цена: 23508.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book offers an interdisciplinary perspective on applying Next Generation Sequencing (NGS) technology to cancer research, surveying the foundations of the technology and its implications in cancer biology, and introducing single cancer cell sequencing.
Автор: Ali Masoudi-Nejad; Zahra Narimani; Nazanin Hossein Название: Next Generation Sequencing and Sequence Assembly ISBN: 1461477255 ISBN-13(EAN): 9781461477259 Издательство: Springer Рейтинг: Цена: 9141.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface.- Next generation sequencing methodologies.- Emergence of next generation sequencing.- Assembly of sequencing data.- De novo assembly algorithms.- Index.
Автор: C. Alexander Valencia; M. Ali Pervaiz; Ammar Husam Название: Next Generation Sequencing Technologies in Medical Genetics ISBN: 1461490316 ISBN-13(EAN): 9781461490319 Издательство: Springer Рейтинг: Цена: 9141.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: High throughput sequencing (HTS) technologies have conquered the genomics and epigenomics worlds. The applications of HTS methods are wide, and can be used to sequence everything from whole or partial genomes, transcriptomes, non-coding RNAs, ribosome profiling, to single-cell sequencing. Having such diversity of alternatives, there is a demand for information by research scientists without experience in HTS that need to choose the most suitable methodology or combination of platforms and to define their experimental designs to achieve their specific objectives. Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing aims to collect in a single volume all aspects that should be taken into account when HTS technologies are being incorporated into a research project and the reasons behind them. Moreover, examples of several successful strategies will be analyzed to make the point of the crucial features. This book will be of use to all scientist that are unfamiliar with HTS and want to incorporate such technologies to their research.
Автор: Naiara Rodr?guez-Ezpeleta; Michael Hackenberg; Ana Название: Bioinformatics for High Throughput Sequencing ISBN: 1489998764 ISBN-13(EAN): 9781489998767 Издательство: Springer Рейтинг: Цена: 22201.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Next generation sequencing is revolutionizing molecular biology. The generation of gigabases of sequence information for each of this huge bandwidth of applications in just a few days makes the development of bioinformatics applications for next generation sequencing data analysis as urgent as challenging.
Автор: Wei Wu; Hani Choudhry Название: Next Generation Sequencing in Cancer Research, Volume 2 ISBN: 3319158104 ISBN-13(EAN): 9783319158105 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Latest generation sequencing revolutionizes the fields of cancer research and oncology. This follow-up volume focuses more extensively on single cell sequencing of cancer and trials in drug resistance. A reasonable cancer treatment plan and potential cure is more optimistic now with the unfolding of the cancer genome.
Rapid technological developments have led to increasingly efficient sequencing approaches. Next Generation Sequencing (NGS) is increasingly common and has become cost-effective, generating an explosion of sequenced data that need to be analyzed. The skills required to apply computational analysis to target research on a wide range of applications that include identifying causes of cancer, vaccine design, new antibiotics, drug development, personalized medicine and higher crop yields in agriculture are highly sought after.
This invaluable book provides step-by-step guides to complex topics that make it easy for readers to perform essential analyses from raw sequenced data to answering important biological questions. It is an excellent hands-on material for teachers who conduct courses in bioinformatics and as a reference material for professionals. The chapters are written to be standalone recipes making it suitable for readers who wish to self-learn selected topics. Readers will gain skills necessary to work on sequenced data from NGS platforms and hence making themselves more attractive to employers who need skilled bioinformaticians to handle the deluge of data.
Автор: Wei Wu; Hani Choudhry Название: Next Generation Sequencing in Cancer Research ISBN: 1489988084 ISBN-13(EAN): 9781489988089 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides an interdisciplinary perspective of applying Next Generation Sequencing (NGS) technology to cancer research. It aims to systematically introduce the concept of NGS, a variety of NGS platforms and their practical implications in cancer biology.This unique and comprehensive text will integrate the unprecedented NGS technology into various cancer research projects as opposed to most books which offer a detailed description of the technology. This volume will present true experimental results with concrete data processing pipelines, discuss the bottleneck of each platform for real project in cancer research. In additional, single cancer cell sequencing as the proof of concept will be introduced in this book, along with cutting-edge information provided will help the intended audience to develop a comprehensive understanding of the NGS technology and practical whole genome sequencing data analysis and rapidly translate into their own research, specifically in the field of cancer biology.
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