Описание: Part I. Screening and Design Purification Strategies
1. Protein Purification: An Overview
Nikolaos Labrou
2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE
Ignacio Amadeo, Laura Mauro, Eduardo Ortн, and Guillermina Forno
3. High Throughput Process Development: I. Process Chromatography
A. S. Rathore and R. Bhambure
4. High Throughput Process Development: II. Membrane Chromatography
A. S. Rathore and S. Muthukumar
5. Media Selection in Ion Exchange Chromatography in a Single Microplate
Charlotte Cabanne and Xavier Santarelli
6. High Throughput Screening of Dye-Ligands for Chromatography
Sunil Kumar and Narayan S. Punekar
7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips
Sunil Chhatre and Daniel G. Bracewell
Part II. Low-Resolution Protein Purification Methods
8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline
Zhengrong GU
9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants
Oscar Aguilar and Marco Rito-Palomares
10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins
A. S. Rathore and R. Bhambure
11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)
Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta
12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm
Ario de Marco
13. Immunoglobulin Purification by Caprylic Acid
Victor Morais, Patricia Berasain, and Hugo Massaldi
Part III: High-Resolution Protein Purification Methods
14. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview
Ana Sofia Pina, Нris L. Batalha, and Ana Cecнlia A. Roque
15. Expression and Purification of Large Active GST Fusion Enzymes
Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa
16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins
Sharon Williams, Phil Morton, and Dev Baines
17. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery
My Hedhammar, Johan Nilvebrant, and Sophia Hober
18. An Orthogonal Fusion Tag for Efficient Protein Purification
Johan Nilvebrant, Mikael Еstrand, and Sophia Hober
19. Phage Display of Engineered Binding Proteins
Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servй W.M. Kengen, and John van der Oost
20. Biomimetic Affinity Ligands for Protein Purification
Isabel T. Sousa and M. Вngela Taipa
21. Synthesis and Application of Dye-Ligand Affinity Adsorbents
Evangelia Chronopoulou and Nikolaos E. Labrou
22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification
S. A. Camperi, M. C. Martнnez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone
23. Affinity Chromatography of Proteins on Monolithic Columns
E.G. Vlakh, G.A. Platonova, and T.B. Tennikova
24. Sample Displacement Batch Chromatography of Proteins
Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlьter
Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization
25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches
Catalin E. Doneanu, Weibin Chen, an
