High-Resolution Profiling of Protein-RNA Interactions, Mathias Munschauer
Автор: Ren-Jang Lin Название: RNA-Protein Complexes and Interactions ISBN: 1493935895 ISBN-13(EAN): 9781493935895 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2
Lindsey Skrdlant and Ren-Jang Lin
2. Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)
Je-Hyun Yoon and Myriam Gorospe
3. Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro
Shuai Hou, Lei Shi, and Haixin Lei
4. Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting
Xuexiu Zheng, Sunghee Cho, Heegyum Moon, Tiing Jen Loh, Hana Jang, and Haihong Shen
5. Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture
Naoyuki Kataoka
6. Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
Keith T. Gagnon
7. In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly
Hua-Lin Zhou and Hua Lou
8. Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases
Ya-Ming Hou
9. Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles
Chao Huang, Guowei Wu, and Yi-Tao Yu
10. Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture
Shangbing Zang and Ren-Jang Lin
11. Measuring mRNA Translation by Polysome Profiling
Marek Kudla and Fedor V. Karginov
12. Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq
Cheryl Stork and Sika Zheng
13. Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment
Sarah F. Mitchell and Roy Parker
14. Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks
Hsin-Sung Yeh, Jae-Woong Chang, and Jeongsik Yong
15. Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking
Fu-Lung Yeh, Luh Tung, and Tien-Hsien Chang
16. Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
Jiehua Zhou and John J. Rossi
17. mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins
Sebastien M. Weyn-Vanhentenryck and Chaolin Zhang
18. Design
Автор: Elliott Michael Название: Molecular Biology of RNA ISBN: 0199288372 ISBN-13(EAN): 9780199288373 Издательство: Oxford Academ Рейтинг: Цена: 6493.00 р. Наличие на складе: Поставка под заказ.
Описание: Molecular Biology of RNA provides a broad survey of RNA structure and function. Describing the life cycle of an RNA, it reveals how hugely diverse gene products stem from a relatively modest gene pool. It also describes how RNA molecules have a range of biological functions beyond messenger RNA, including catalysts and regulatory molecules.
Название: Rna interference ISBN: 3540751564 ISBN-13(EAN): 9783540751564 Издательство: Springer Рейтинг: Цена: 28734.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In the last few years the major effect that RNAi has had in invertebrate systems is beginning to take hold in mammalian systems through both single gene knockdown experiments and genome-scale screens. Through the introduction of RNAi, mammalian systems have finally gained admittance to the pantheon of model genetic systems.
Автор: Mathias Munschauer Название: High-Resolution Profiling of Protein-RNA Interactions ISBN: 3319162527 ISBN-13(EAN): 9783319162522 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universit?t Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.
Автор: J?rg Epplen; Thomas Lubjuhnn Название: DNA Profiling and DNA Fingerprinting ISBN: 3764360186 ISBN-13(EAN): 9783764360184 Издательство: Springer Рейтинг: Цена: 13060.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This text reflects practical approaches to answering a variety of biological and medical questions, using DNA fingerprinting and genetic profiling in a broad sense that also includes statistical evaluation of the data. The book is aimed at students as well as academic teachers and researchers.
Автор: Marvin Jens Название: Dissecting Regulatory Interactions of RNA and Protein ISBN: 3319070819 ISBN-13(EAN): 9783319070810 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The work described in this book is an excellent example of interdisciplinary research in systems biology. It shows how concepts and approaches from the field of physics can be efficiently used to answer biological questions and reports on a novel methodology involving creative computer-based analyses of high-throughput biological data. Many of the findings described in the book, which are the result of collaborations between the author (a theoretical scientist) and experimental biologists and between different laboratories, have been published in high-quality peer-reviewed journals such as Molecular Cell and Nature. However, while those publications address different aspects of post-transcriptional gene regulation, this book provides readers with a complete, coherent and logical view of the research project as a whole. The introduction presents post-transcriptional gene regulation from a distinct angle, highlighting aspects of information theory and evolution and laying the groundwork for the questions addressed in the subsequent chapters, which concern the regulation of the transcriptome as the primary functional carrier of active genetic information.
Автор: Marvin Jens Название: Dissecting Regulatory Interactions of RNA and Protein ISBN: 3319361171 ISBN-13(EAN): 9783319361178 Издательство: Springer Рейтинг: Цена: 15672.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The introduction presents post-transcriptional gene regulation from a distinct angle, highlighting aspects of information theory and evolution and laying the groundwork for the questions addressed in the subsequent chapters, which concern the regulation of the transcriptome as the primary functional carrier of active genetic information.
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