Описание: A Safety Considerations Genomie sequencing involves a number oj hazard- ous steps, such as high eurrent, high voltage, radioaetive and highly toxie chemieals. It is, the- jore, absolutely essential that the instruetions oj equipment manu/aeturers bejollowed and that par- tieular attention is paid to the loeal and jederal safety regulations. I Introduction 13 B Introduction Hypomethylation ofDNA has been positively correlated with thc activation of many eucaryotic genes. During the transition from inactive to active genes changes in the protein/DNA interaction pattern occur. Tran- scriptional activation of eucaryotic genes is mediated by specific interac- tions oftransacting factors with their respective DNA binding sites in Lhe control regions (promoters, enhancers) ofthe genes. This process is ofLen accompanied by changes in local chromatin strucLure, witnessed by the appearance of nuclease hypersensitive sites, as weil as by changes in protein-DNA interactions and, in the case of higher eucaryotes, alterations ofthe cytosine methylation pattern. The sole available experimental tech- nique that permits the study ofthe latter phenomena at single nucleotide resolution is direct genomic sequencing/footprinting, pioneered by Church and Gilbert (1984). This method combines the chemical DNA- sequencing procedure of Maxam amI Gilbert (1980) with thc detection 01' DNA sequences by electroblotting and indirect end-Iabeling by hybridiza- tl0n. An alternative possibility is the novel procedure (Saluz and . lost, 1989), using Taq polymerase. The first steps 01' both meLhods are essen- tially the same: total genomic DNA is digested wiLh a suilable restriction enzyme and the resulting DNA fragments are chemically sequeneed.
Описание: Since it has been demonstrated that reducing the steady-state plasma LDL-cholesterol concentration produces a proportional decrease in the risk of developing atherosclerosis 2), a major effort is now underway to develop safe and effective lipid lowering agents.
Автор: Fu Haian Название: Protein-Protein Interactions / Methods and Applications ISBN: 1588291200 ISBN-13(EAN): 9781588291202 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A collection of highly successful biochemical, biophysical, genetic, and computational techniques for studying protein-protein interactions. These readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions in vitro or in vivo, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics and bioinformatics-based approaches, such as the Scansite program for computational analysis. These powerful tools are essential for deciphering how proteins interact with each other to form biological networks, as well as for unraveling protein-protein interactions in disease to find novel therapeutic targets.
Описание: Proceedings of a NATO ARW held in Cargese, Corsica, France, June 1-5, 1993
Автор: Mark C. Williams; L. James Maher, III Название: Biophysics of DNA-Protein Interactions ISBN: 1461426952 ISBN-13(EAN): 9781461426950 Издательство: Springer Рейтинг: Цена: 16977.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Focusing on the biophysics of DNA-protein interactions, this volume presents new methods and results that explore the mechanism behind prtein and DNA interaction. Recent advances in biophysics are discussed, in addition to proteins that alter DNA properties to faciliate interactions for transcription or replication.
Автор: Enno Klussmann; John Scott Название: Protein-Protein Interactions as New Drug Targets ISBN: 3642091946 ISBN-13(EAN): 9783642091940 Издательство: Springer Рейтинг: Цена: 65305.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book covers therapeutically relevant protein-protein interactions, focusing on scaffolding proteins tethering signal transduction processes to defined cellular compartments by direct protein-protein interactions. Recent advances in the field are included.
Автор: K.W.A. Wirtz; Lester Packer; J.?. Gustafsson; A.E. Название: New Developments in Lipid-Protein Interactions and Receptor Function ISBN: 1461362393 ISBN-13(EAN): 9781461362395 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Proceedings of a NATO ASI held in Spetsai, Greece, August 16-27, 1992
Автор: Gabriel Waksman Название: Proteomics and Protein-Protein Interactions ISBN: 1489996117 ISBN-13(EAN): 9781489996114 Издательство: Springer Рейтинг: Цена: 28732.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Gabriel Waksman Institute of Structural Molecular Biology, Birkbeck and University College London, Malet Street, London WC1E 7HX, United Kingdom Address for correspondence: Professor Gabriel Waksman Institute of Structural Molecular Biology Birkbeck and University College London Malet Street London WC1E 7H United Kingdom Email: g.
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