Functional Glycomics, Jianjun Li

Автор: Gordan Lauc
Название: High-Throughput Glycomics and Glycoproteomics
ISBN: 1493964917 ISBN-13(EAN): 9781493964918
Издательство: Springer
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Цена: 18167.00 р.
Наличие на складе: Нет в наличии.

Описание: This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.

Автор: Jianjun Li
Название: Functional Glycomics
ISBN: 1493956973 ISBN-13(EAN): 9781493956975
Издательство: Springer
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Цена: 19564.00 р.
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Описание: The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.

Автор: Nicolle H. Packer; Niclas G. Karlsson
Название: Glycomics
ISBN: 1617377694 ISBN-13(EAN): 9781617377693
Издательство: Springer
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Цена: 20263.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: To aid scientists in a vital subject, this compendium of laboratory protocols reflects the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools designed for the analysis of glycosylation.