Free Radicals and Antioxidant Protocols, Rao M. Uppu; Subramanyam N. Murthy; William A. Pry

Автор: Armstrong Donald
Название: Oxidative Stress Biomarkers and Antioxidant Protocols
ISBN: 0896038505 ISBN-13(EAN): 9780896038509
Издательство: Springer
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Цена: 21661.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Автор: Halliwell
Название: Free Radicals in Biology & Medicine
ISBN: 0198500440 ISBN-13(EAN): 9780198500445
Издательство: Oxford Academ
Цена: 7128.00 р.
Наличие на складе: Поставка под заказ.

Описание: Since the publication of the second edition of "Free Radicals in Biology and Medicine" in 1989 the techniques of modern molecular, cellular, and structural biology have provided new data that have transformed scientific thinking about topics within free radical research. Reactive species, such as peroxynitrite, have been discovered to have a role in biological systems. Therefore much of the third edition has been completely rewritten and what remains from the second has been thoroughly updated. There are new chapters on antioxidant defence systems in bacteria, plants, and animals; the detection of free radicals; the role of antioxidants in ageing, nutrition, disease, and therapy; and a new appendix on molecular biology. The chapter on oxidative stress and its consequences includes the damage and repair of DNA, proteins, and lipids, cytokines, and cell death (both apoptosis and necrosis). Free radicals and other reactive species are now implicated in a wide range and number of diseases and the third edition covers their role in hypertension, diabetes, lung damage, cystic fibrosis, nervous system disorders, viral infections, and cancer. This book is intended for postgraduate researchers, academics, industrial scientists, clinicians, and medical workers in chemistry, biochemistry and biomedical fields.