Coronavirus Replication and Reverse Genetics, Luis Enjuanes

Автор: Sunil K. Lal
Название: Molecular Biology of the SARS-Coronavirus
ISBN: 364242435X ISBN-13(EAN): 9783642424359
Издательство: Springer
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Цена: 39130.00 р.
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Описание: SARS was the ?rst new plague of the twenty-?rst century. Within months, it spread worldwide from its “birthplace” in Guangdong Province, China, affecting over 8,000 people in 25 countries and territories across ?ve continents. SARS exposed the vulnerability of our modern globalised world to the spread of a new emerging infection. SARS (or a similar new emerging disease) could neither have spread so rapidly nor had such a great global impact even 50 years ago, and arguably, it was itself a product of our global inter-connectedness. Increasing af?uence and a demand for wild-game as exotic food led to the development of large trade of live animal and game animal markets where many species of wild and domestic animals were co-housed, providing the ideal opportunities for inter-species tra- mission of viruses and other microbes. Once such a virus jumped species and attacked humans, the increased human mobility allowed the virus the opportunity for rapid spread. An infected patient from Guangdong who stayed for one day at a hotel in Hong Kong led to the transmission of the disease to 16 other guests who travelled on to seed outbreaks of the disease in Toronto, Singapore, and Vietnam, as well as within Hong Kong itself. The virus exploited the practices used in modern intensive care of patients with severe respiratory disease and the weakness in infection control practices within our health care systems to cause outbreaks within hospitals, further amplifying the spread of the disease. Health-care itself has become a two-edged sword.

Автор: Monika Glinkowska; Lidia Boss; Grzegorz Wegrzyn
Название: DNA Replication Control in Microbial Cell Factories
ISBN: 3319105329 ISBN-13(EAN): 9783319105321
Издательство: Springer
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Цена: 9141.00 р.
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Описание: Introduction.- Coordinating duplication of genetic material with cell growth - current views and old questions.- Structural organization of cellular processes and the regulation of cell cycle.- The link between metabolism and DNA replication.- Transcriptional activation - the unsolved problem.- Activity of DnaA is inhibited by components of translation machinery.- Nucleoid-associated proteins modulate activity of the DnaA initiator.- Interaction of DnaA with acidic components of fluid cellular membrane regulates its nucleotide-bound status and binding to oriC.- Influence of DNA replication on cell structure and function.- Conclusions.

Автор: Craig E. Cameron; Matthias Gotte; Kevin Raney
Название: Viral Genome Replication
ISBN: 1489984682 ISBN-13(EAN): 9781489984685
Издательство: Springer
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Цена: 32651.00 р.
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Описание: This book provides the first comprehensive review of viral genome replication strategies, emphasizing not only pathways and regulation but also the structure-function, mechanism, and inhibition of proteins and enzymes required for this process.

Автор: Leyi Wang
Название: Animal Coronaviruses
ISBN: 1493934120 ISBN-13(EAN): 9781493934126
Издательство: Springer
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Цена: 18167.00 р.
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Описание: It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses.

Автор: Helena Jane Maier; Erica Bickerton; Paul Britton
Название: Coronaviruses
ISBN: 1493924370 ISBN-13(EAN): 9781493924370
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.