Описание: Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins. Many cellular processes involve special proteins that bind to specific DNA sites with high affinity. How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DNA at specific sites and indicate that DNA deformability is a key factor in site-specific recognition. However, the dynamics of DNA deformations have been difficult to capture, thus obscuring our understanding of recognition mechanisms. The experiments presented in this thesis uncover, for the first time, rapid (~100-500 microseconds) DNA unwinding/bending attributed to nonspecific interrogation, prior to slower (~5-50 milliseconds) DNA kinking/bending/nucleotide-flipping during recognition. These results help illuminate how a searching protein interrogates DNA deformability and eventually “stumbles” upon its target site. Submillisecond interrogation may promote preferential stalling of the rapidly scanning protein at cognate sites, thus enabling site-recognition. Such multi-step search-interrogation-recognition processes through dynamic conformational changes may well be common to the recognition mechanisms for diverse DNA-binding proteins.
Автор: N. Ulfig Название: Calcium-Binding Proteins in the Human Developing Brain ISBN: 3540434631 ISBN-13(EAN): 9783540434634 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In the mature brain calcium ions play pivotal roles in transmembrane and intracellular transmission of signals. Thus, calcium is involved in numerous neuronal functions, and this text explores the complex processes dependent on and modulated by calcium.
Автор: Claus W. Heizmann Название: Calcium-Binding Proteins and RAGE ISBN: 149395976X ISBN-13(EAN): 9781493959761 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology (TM) book reviews common techniques for studying calcium binding proteins, underlining the diagnostic and clinical importance of this family of proteins. Offers materials lists, protocols, troubleshooting advice and more.
Автор: Claus W. Heizmann Название: Novel Calcium-Binding Proteins ISBN: 3642761526 ISBN-13(EAN): 9783642761522 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A wide variety of hormones, neurotransmitters and growth factors exert their cellular effects by reacting first with membrane receptors resulting in an increase of intracellular calcium and the cellular response.
Автор: Charles Gerday; L. Bolis; R. Gilles Название: Calcium and Calcium Binding Proteins ISBN: 3642730442 ISBN-13(EAN): 9783642730443 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A Specialized Symposium Organized for the European Society for Comparative Physiology and Biochemistry in Crans-sur- Sierre, Switzerland, September 16-18, 1986
Автор: J. Robin Harris Название: Cholesterol Binding and Cholesterol Transport Proteins: ISBN: 9048186218 ISBN-13(EAN): 9789048186211 Издательство: Springer Рейтинг: Цена: 36570.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Knowledge of cholesterol and its interaction with protein molecules is of fundamental importance. This books examines the structural and functional aspects of the families of cholesterol-binding and cholesterol transport proteins.
Автор: Robert K. Poole Название: Recent Advances in Microbial Oxygen-Binding Proteins,67 ISBN: 0128032987 ISBN-13(EAN): 9780128032985 Издательство: Elsevier Science Рейтинг: Цена: 20886.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This latest volume in Advances in Microbial Physiology continues the long tradition of topical and important reviews in microbiology. Contains contributions from leading authorities in the field of microbial physiology Informs and updates on all the latest developments in the field
Автор: James L. Keck Название: Single-Stranded DNA Binding Proteins ISBN: 1493962515 ISBN-13(EAN): 9781493962518 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology (TM) book offers techniques for examining fundamental properties of SSBs and for exploiting the biochemical functions of SSBs as in vitro and in vivo reagents. Includes materials lists, protocols, troubleshooting tips and more.
Автор: Barry L. Stoddard Название: Computational Design of Ligand Binding Proteins ISBN: 1493935674 ISBN-13(EAN): 9781493935673 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today`s leaders in the field ofprotein engineering.
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