Oxidative Stress Biomarkers and Antioxidant Protocols, Donald Armstrong

Автор: Armstrong Donald
Название: Oxidative Stress Biomarkers and Antioxidant Protocols
ISBN: 0896038505 ISBN-13(EAN): 9780896038509
Издательство: Springer
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Описание: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Автор: Rao M. Uppu; Subramanyam N. Murthy; William A. Pry
Название: Free Radicals and Antioxidant Protocols
ISBN: 1617796832 ISBN-13(EAN): 9781617796838
Издательство: Springer
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Описание: This cutting-edge and updated book offers methods for the rapid detection of RONs and redox stress. It includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers.

Автор: Donald Armstrong
Название: Advanced Protocols in Oxidative Stress III
ISBN: 1493914405 ISBN-13(EAN): 9781493914401
Издательство: Springer
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Описание: Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.

Автор: Sami Ahmad
Название: Oxidative Stress and Antioxidant Defenses in Biology
ISBN: 1461596912 ISBN-13(EAN): 9781461596912
Издательство: Springer
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Описание: The ground-state of molecular oxygen, O, is essential to many 2 indispensible metabolic processes of all aerobic life forms ranging from prokaryotes, protists, plants, and fungi to animals. Research by mammalian toxicologists and clinicians has unravelled persua- sive evidence that O dependence imposes universal toxicity to all 2 aerobic life processes. The basis of this paradox is that one-electron reduction of O2 generates the superoxide anion free radical, 0;-, from numerous biological sources; for example, redox-active autoxidizable molecules such as catecholamines, oxidoreductases, and subcellular organelles such as mitochondria, endoplasmic reti'C- ulum (microsomes), nuclei, and chloroplasts. Oxygen is also acti- vated in biologically relevant photosensitizing reactions to highly re- active singlet oxygen, 10, 2 In all biological systems, 0;- undergoes further reduction to H 0 2 2 via Fenton reaction to the hydroxyl radical, -OH. These, and some other forms of activated O, constitute reactive oxygen species (ROS) 2 and/or metabolites (ROM). Both 'OH and 10 are the most reactive 2 forms of ROS known and among their deleterious reactions are ox- idation of proteins, DNA, steroidal compounds, and peroxidation of the cell membrane's unsaturated lipids to form unstable hydro- peroxides. Their many breakdown products include malondialde- hyde and hydroxynonenals that are themselves highly reactive and threaten cellular integrity and function. More importantly, they de- compose to free radicals that can continue to propagate the vicious lipid peroxidation chain reaction. This is the so-called endogenous oxidative stress with which all aerobic organisms must cope.

Автор: Tomris ?zben
Название: Free Radicals, Oxidative Stress, and Antioxidants
ISBN: 0306458136 ISBN-13(EAN): 9780306458132
Издательство: Springer
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Описание: Proceedings of a NATO ASI held in Antalya, Turkey, May 24-June 4, 1997

Автор: Huangxian Ju
Название: Immunosensing for Detection of Protein Biomarkers
ISBN: 0081019998 ISBN-13(EAN): 9780081019993
Издательство: Elsevier Science
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Описание:

Immunosensing for Detection of Protein Biomarkers not only introduces the principles, methods, and classification of immunoassay, but also presents the latest achievements in areas such as electrochemical immunosensors, nanoprobe-based immunoassay, chemiluminescence immunoassay, electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging for immunoassay, signal amplification for immunoassay, and so on.

Автор: Donald Armstrong
Название: Advanced Protocols in Oxidative Stress III
ISBN: 1493946404 ISBN-13(EAN): 9781493946402
Издательство: Springer
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Описание:

Part I: Reactive Oxygen and Nitrogen Techniques

1. Derivatization and Detection of Small Aliphatic and Lipid-Bound Carbonylated Lipid Peroxidation Products by ESI-MS

Ivana Milic and Maria Fedorova

2. Specific Imaging and Tracking of Mitochondria in Live Cells by a Photostable AIE Luminogen

Chris W.T. Leung, Yuning Hong, and Ben Zhong Tang

3. Analysis of Relationship Between Oxidized Phospholipid Structure and Interaction with the Class B Scavenger Receptors

Detao Gao, Larry W. Sayre, and Eugene A. Podrez

4. A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA

Takayuki Shibamoto

5. Nitric Oxide Availability as a Marker of Oxidative Stress

Dan Pierini and Nathan S. Bryan

6. Quantification of DNA Repair Capacity Towards Oxidatively Damaged DNA in Subcellular and Cellular Systems by a Non-Radioactive Cleavage Assay

Ingrit Hamann and Andrea Hartwig

7. Chiral Phase-HPLC Separation of Hydroperoxyoctadecenoic Acids and Their Biosynthesis by Fatty Acid Dioxygenases

Ernst H. Oliw and Anneli Wennman

8. Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and H₂S Repair

Zhangrong Lou, Peng Li, and Keli Han

9. Evaluation of Physical Integrity of Lipid Bilayer Under Oxidative Stress: Application of Fluorescence Microscopy and Digital Image Processing

Ran Liang, Jian-Ping Zhang, and Leif H. Skibsted

10. Electrochemical Detection of Glutathione S-Transferase: An Important Enzyme in the Cell Protective Mechanism Against Oxidative Stress

Manuel C. Martos-Maldonado, Juan M. Casas-Solvas, Antonio Vargas-Berenguel, and Luis Garcнa-Fuentes

11. Using Nanosensors for In Situ Monitoring and Measurement of Nitric Oxide and Peroxynitrite in a Single Cell

Tadeusz Malinski

Part II: Antioxidant Technology and Application

12. Curcuma longa Attenuates Carbon Tetrachloride-Induced Oxidative Stress in T Lymphocyte Subpopulations

H.A. Abu-Rizq, Mohamed H. Mansour, and Mohammad Afzal

13. Prolongation of the Lag Time Preceding Peroxidation of Serum Lipids: A Measure of Antioxidant Capacity

Ilya Pinchuk and Dov Lichtenberg

14. Identification of (Anti-Oxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements

Eric Deconinck, Deborah Custers, and Jacques Omer De Beer

15. Simultaneous Determination of Ascorbic Acid, Aminothiols, and Methionine in Biological Matrices Using Ion-Pairing RP-HPLC Coupled with Electrochemical Detector

Muhammad Imran Khan, Zafar Iqbal, and Abad Khan

16. Portable Nanoparticle Based Sensors for Antioxidant Analysis

Erica Sharpe and Silvana Andreescu

17. Fe³⁺-Fe²⁺ Transformation Method: An Important Antioxidant Assay

İlhami Gьlзin

18. Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity

Augustin C. Mot, Cristina Bischin, Grigore Damian, and Radu Silaghi-Dumitrescu

19. Computational Studies on Conformation, Electron Density Distributions, and Antioxidant Properties of Anthocyanidins

Ricardo A. Mosquera, Laura Estйvez, and Mercedes Garcнa-Bugarнn

20. Automatic Flow Injection Analysis (FIA) Determination of Total Reducing Capacity in Serum and Urine Samples

Marcela A. Segundo, Ildikу V. Tуth, Luнs M. Magalhгes, and Salette Reis

21. Mass Spectrometry Detection of Isolevuglandin Adduction to Specific P

Автор: Denys J. Charles
Название: Antioxidant Properties of Spices, Herbs and Other Sources
ISBN: 1489994785 ISBN-13(EAN): 9781489994783
Издательство: Springer
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Описание: The scientific world and modern society today is experiencing the dawning of an era of herbal medicine. Extensive research has shown that aromatic plants are important anti-inflammatory, antioxidant, anti aging and immune boosting delectable foods, with the magic and miracle to boost our immune system providing us with extended and an improved quality of life. Apart from making bland recipes into welcoming or interesting victories, herbs and spices have stirred the minds of the research community to look deeper into its active components from a functional perspective. It is essential to present the scientific and medicinal aspect of herbs and spices together with the analysis of constituents, its medicinal application, toxicology and its physiological effects. Herbs and spices with high levels of antioxidants are in great demand as they tend to promote health and prevent diseases naturally assuring increased safety and reliability for consumers. Herbs and spices are not only known for taste and flavor, but today research has opened up a new realm in which the antioxidant properties of these aromatic plants provide preservation for foods and health benefits for consumers who look forward to concrete scientific research to guide them further and explore herbal medicine. The aim of this book is to create awareness in society about the reliability of medicinal properties of certain herbs and spices through scientific and scholarly research.

Автор: Harndan
Название: Cancer Biomarkers. 2007
ISBN: 0471745162 ISBN-13(EAN): 9780471745167
Издательство: Wiley
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Описание: Tools, techniques, and progress in cancer biomarkers discovery The completion of a number of gene sequencing projects, recent advances in genomic and proteomic technologies, and the availability of powerful bioinformatics tools have led to promising new avenues and approaches in the search for cancer biomarkers.

Автор: Donald Armstrong
Название: Advanced Protocols in Oxidative Stress II
ISBN: 1493957031 ISBN-13(EAN): 9781493957033
Издательство: Springer
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Описание: This second volume of Advanced Protocols in Oxidative Stress focuses on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data.

Автор: Adrianne Bendich; Marshall Phillips; Robert P. Ten
Название: Antioxidant Nutrients and Immune Functions
ISBN: 1461278635 ISBN-13(EAN): 9781461278634
Издательство: Springer
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Описание: Proceedings of the Agricultural and Food Chemistry Division of the American Chemical Society Symposium held in Los Angeles, California, September 29, 1988