Xenopus Protocols, X. John? Liu

Автор: G. Bernardini; M. Prati; E. Bonetti; G. Scari
Название: Atlas of Xenopus Development
ISBN: 8847029074 ISBN-13(EAN): 9788847029071
Издательство: Springer
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Цена: 15672.00 р.
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Описание: Developmental biology attracts scientists from many different areas of biology, and the amphibian Xenopus holds a special place among the organisms studied as a model of vertebrate development.

Автор: Donald Armstrong
Название: Advanced Protocols in Oxidative Stress III
ISBN: 1493914405 ISBN-13(EAN): 9781493914401
Издательство: Springer
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Цена: 25155.00 р.
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Описание: Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.

Автор: Armstrong Donald
Название: Oxidative Stress Biomarkers and Antioxidant Protocols
ISBN: 0896038505 ISBN-13(EAN): 9780896038509
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Автор: Phouthone Keohavong; Stephen G. Grant
Название: Molecular Toxicology Protocols
ISBN: 1627037381 ISBN-13(EAN): 9781627037389
Издательство: Springer
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Цена: 30745.00 р.
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Описание: This book details techniques for analyzing genotoxic exposure and the resulting biological effects, including intermediate biomarkers such as DNA and chromosomal damage, mutations in reporter and oncogenes, and the earliest possible detection of cancer.

Автор: Aguilar Marie-Isabel
Название: HPLC of Peptides and Proteins / Methods and Protocols
ISBN: 0896039773 ISBN-13(EAN): 9780896039773
Издательство: Springer
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Цена: 23757.00 р.
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Описание: Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.