MicroRNA Protocols, Shao-Yao Ying

Автор: Zhang
Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
ISBN: 1607617684 ISBN-13(EAN): 9781607617686
Издательство: Springer
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Цена: 22359.00 р.
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Описание: This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.

Автор: Rani
Название: MicroRNA Profiling
ISBN: 1493965220 ISBN-13(EAN): 9781493965229
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

Автор: Zhiguo Wang; Baofeng Yang
Название: MicroRNA Expression Detection Methods
ISBN: 3642425186 ISBN-13(EAN): 9783642425189
Издательство: Springer
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Цена: 28732.00 р.
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Описание: This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression as well as their application in miRNA research and their potential as tools for clinical diagnosis and prognosis.

Автор: Baohong Zhang; Edmund J. Stellwag
Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
ISBN: 149395749X ISBN-13(EAN): 9781493957491
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.

Автор: Zhiguo Wang
Название: MicroRNA Interference Technologies
ISBN: 3642101518 ISBN-13(EAN): 9783642101519
Издательство: Springer
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Цена: 23508.00 р.
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Описание: This book presents an overview of up-to-date knowledge of MicroRNAs (miRNAs) biology and their potential as therapeutic targets for human disease. It also gives an introduction of the new concept of miRNA interference (miRNAi).

Автор: Mettinger
Название: Exosomes, Stem Cells and MicroRNA
ISBN: 3319744690 ISBN-13(EAN): 9783319744698
Издательство: Springer
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Цена: 22359.00 р.
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Описание: This volume provides insight into the pivotal roles of stem cells, exosomes and other microvesicles in biofunction and molecular mechanisms and their therapeutic potential in translational nanomedicine. It further highlights evidence from recent studies as to how stem cell derived exosomes and microRNAs may restore and maintain tissue homeostasis, enable cells to recover critical cellular functions and begin repair regeneration. These early studies in animal models of aging also show evidence of improved immune, cardiovascular and cognitive functions as well as improved health span and life span. The use of exosomes from body fluids to define specific biomarkers for various tumors may also clear the path to patient-targeted treatments by developing exosome-derived microRNA based cancer therapeutics. It is essential reading for graduate students, research fellow and biomedical researchers in academia or the pharmaceutical or biotech industries.

Автор: Karl L. Mettinger; Pranela Rameshwar; Vinod Kumar
Название: Exosomes, Stem Cells and MicroRNA
ISBN: 3030089991 ISBN-13(EAN): 9783030089993
Издательство: Springer
Рейтинг:
Цена: 22359.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume provides insight into the pivotal roles of stem cells, exosomes and other microvesicles in biofunction and molecular mechanisms and their therapeutic potential in translational nanomedicine. It further highlights evidence from recent studies as to how stem cell derived exosomes and microRNAs may restore and maintain tissue homeostasis, enable cells to recover critical cellular functions and begin repair regeneration. These early studies in animal models of aging also show evidence of improved immune, cardiovascular and cognitive functions as well as improved health span and life span. The use of exosomes from body fluids to define specific biomarkers for various tumors may also clear the path to patient-targeted treatments by developing exosome-derived microRNA based cancer therapeutics. It is essential reading for graduate students, research fellow and biomedical researchers in academia or the pharmaceutical or biotech industries.

Автор: Zengrong Liu; Jianwei Shen; Shuiming Cai; Fang Yan
Название: MicroRNA Regulatory Network: Structure and Function
ISBN: 9402415750 ISBN-13(EAN): 9789402415759
Издательство: Springer
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Цена: 20962.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book discusses topics related to the topological structure and biological function of gene networks regulated by microRNAs. It focuses on analyzing the relation between topological structure and biological function, applying these theoretical results to gene networks involving microRNA, illustrating their biological mechanisms, and identifying the roles of microRNA in controlling various phenomena emerging from the networks. In addition, the book explains how to control the complex biological phenomena using mathematical tools and offers a new perspective on studying microRNA. It is a useful resource for graduate students and researchers who are working on or interested in microRNAs and gene network.