Описание: 1. Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder Sophie S Abby and Eduardo PC Rocha
2. Protein Sorting Prediction
Henrik Nielsen
3. Cell Fractionation
Melissa Petiti, Laetitia Houot, and Denis Duchй
4. Defining Lipoprotein Localisation by Fluorescence Microscopy
Maria Guillermina Casabona, Mylиne Robert-Genthon, Didier Grunwald, and Ina Attrйe
5. Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate
Nienke Buddelmeijer
6. Defining Membrane Protein Localization by Isopycnic Density Gradients
Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow
7. Components Sub-cellular Localisation: Cell Surface Exposure
Anna Konovalova
8. Probing Inner Membrane Protein Topology by Proteolysis
Maxence S. Vincent and Eric Cascales
9. Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM)
Mikhail Bogdanov
10. Defining Membrane Protein Topology using pho-lac Reporter Fusions
Gouzel Karimova and Daniel Ladant
11. In vivo and in vitro Protein-peptidoglycan Interactions
Gang Li and S. Peter Howard
12. Measure of Peptidoglycan Hydrolase Activity
Yoann G. Santin and Eric Cascales
13. Protein-protein Interaction: Bacterial Two-Hybrid
Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant
14. Protein-protein Interactions: Yeast two-hybrid
Jer-Sheng Lin and Erh-Min Lai
15. Protein-protein Interactions: Cytology Two-hybrid
Krishnamohan Atmakuri
16. Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes
Laureen Logger, Abdelrahim Zoued, and Eric Cascales
17. Protein-protein Interactions: Co-immunoprecipitation
Jer-Sheng Lin and Erh-Min Lai
18. Protein-protein Interaction: Tandem Affinity Purification in Bacteria
Julie P.M. Viala and Emmanuelle Bouveret
19. Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes
Raffaele Ieva
20. Protein-protein Interactions: Pull-down Assays
Arthur Louche, Suzana P. Salcedo, and Sarah Bigot
21. Protein-protein interactions: Surface Plasmon Resonance
Badreddine Douzi
22. Assessing Energy-dependent Protein Conformational Changes in the TonB System
Ray A. Larsen
23. Defining Assembly Pathways by Fluorescence Microscopy
Abdelrahim Zoued and Andreas Diepold
24. Large Complexes: Cloning Strategy, Production, and Purification
Eric Durand and Roland Lloubes
25. Shearing and Enrichment of Extracellular Type IV Pili
Alba Katiria, Gonzalez Rivera, and Katrina T. Forest
26. Blue Native PAGE Analysis of Bacterial Secretion Complexes
Susann Zilkenat, Tobias Dietsche, Julia V. Monjarбs Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner
27. In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography
Gregor L. Weiss, Joгo M. Medeiros, and Martin Pilhofer
28. Structural Analysis of Protein Complexes by Cryo Electron Microscopy
Tiago R. D. Costa, Athanasios Ignatiou, and Elena V. Orlova
29. Bacterial Filamentous Appendages I
