Basic Cloning Procedures, Valdis Berzins

Автор: Spangler
Название: Methods in Molecular Biology & Protein Chemistry - Cloning & Characterization of an Enterotoxin Subunit
ISBN: 0470843608 ISBN-13(EAN): 9780470843604
Издательство: Wiley
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Описание: The advances made in molecular biology have allowed scientists to manipulate DNA. This means that molecular biology has become a tool for answering scientific questions that may be quite unrelated to genetics and cell biology. The techniques have become a means to an end, rather than an end in themselves. This work deals with this area.

Автор: Hardin
Название: Cloning Gene Expression
ISBN: 0195132947 ISBN-13(EAN): 9780195132946
Издательство: Oxford Academ
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Описание: The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyzse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, southern and western blotting and cominatorial techniques are empsized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergraduate students, beginning graduates, and practising professionals engaged in a broad range of pursuits. Each experiment is accompanied by detailed process rationale.

Автор: Joel F. Habener
Название: Molecular Cloning of Hormone Genes
ISBN: 146129178X ISBN-13(EAN): 9781461291787
Издательство: Springer
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Цена: 8384.00 р.
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Описание: In addition to provid- ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors.

Автор: Bing-Yuan Chen; Harry W. Janes
Название: PCR Cloning Protocols
ISBN: 1617372811 ISBN-13(EAN): 9781617372810
Издательство: Springer
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Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White`s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.

Автор: R.D. O`Brien
Название: General Principles and Procedures
ISBN: 1468409816 ISBN-13(EAN): 9781468409819
Издательство: Springer
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Описание: The study of these molecules, which may well include such diverse items as the receptors for hormones, neurohumors, pheromones, taste, and many other chemical signals, have a great deal in common, so that the student of any one of them will wish to know the status of research about the others.

Автор: J. Jespersen; R.M. Bertina; F. Haverkate
Название: ECAT Assay Procedures A Manual of Laboratory Techniques
ISBN: 9401053308 ISBN-13(EAN): 9789401053303
Издательство: Springer
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Описание: This book offers a description of current and recently developed laboratory assays in the field of haemostasis and thrombosis. Included were patients with angina pectoris at risk from myocardial infarction, patients undergoing angioplasty at risk from re-stenosis, and patients receiving hip replacement at risk from deep venous thrombosis.

Автор: J.A. Washington
Название: Laboratory Procedures in Clinical Microbiology
ISBN: 1461295505 ISBN-13(EAN): 9781461295501
Издательство: Springer
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Описание: With contributions by Members of the Section of Clinical Microbiology, Department of Laboratory Medicine Mayo Clinic, Rochester, Minnesota

Автор: Zneimer
Название: Cytogenetic Laboratory Management - Chromosomal, FISH, and Microarray-Based Best Practices and Procedures
ISBN: 1119069742 ISBN-13(EAN): 9781119069744
Издательство: Wiley
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Описание: Cytogenetic Laboratory Management: Chromosomal, FISH and Microarray-Based Best Practices and Procedures is a practical guide that describes how to develop and implement best practice processes and procedures in the genetic laboratory setting.