Автор: Robert Damoiseaux; Samuel Hasson Название: Reporter Gene Assays ISBN: 1493992686 ISBN-13(EAN): 9781493992683 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Поставка под заказ.
Описание:
This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.
Автор: Don Anson Название: Reporter Genes ISBN: 1627038620 ISBN-13(EAN): 9781627038621 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments.
Автор: Carl-Fredrik Mandenius; James A. Ross Название: Cell-Based Assays Using iPSCs for Drug Development and Testing ISBN: 149399476X ISBN-13(EAN): 9781493994762 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Поставка под заказ.
Описание:
The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.
Автор: Carl-Fredrik Mandenius; James A. Ross Название: Cell-Based Assays Using iPSCs for Drug Development and Testing ISBN: 1493994794 ISBN-13(EAN): 9781493994793 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Поставка под заказ.
Описание: The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.
Автор: Mohan C. Vemuri Название: Stem Cell Assays ISBN: 1627039376 ISBN-13(EAN): 9781627039376 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of “transplant-ready” or “clinical-grade” stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, through polymerase chain reaction approaches following transplantation, and more. The scientific progress that makes stem cell biology so exciting also threatens to suffocate researchers with an avalanche of information. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, the book Stem Cell Assays aims to present scientific advances in stem cell methods for a wider use by novice and expert scientists, through the Methods in Molecular Biology series.
Автор: Mohan C Vemuri; Lucas G. Chase; Mahendra S. Rao Название: Mesenchymal Stem Cell Assays and Applications ISBN: 1493957791 ISBN-13(EAN): 9781493957798 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book shows how to propagate, characterize, and engineer this special cell type. Using the Methods in Molecular Biology (TM) format, each chapter offers an introduction, step-by-step methods, materials list, and tips on troubleshooting and avoiding pitfalls.
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