Handbook of elispot, 

Автор: Kalyuzhny, Alexander E
Название: Handbook of ELISPOT methods and protocols
ISBN: 1588294692 ISBN-13(EAN): 9781588294692
Издательство: Springer
Цена: 11872.00 р.
Наличие на складе: Поставка под заказ.

Описание: In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

Автор: Kalyuzhny
Название: Handbook of ELISPOT
ISBN: 1493985663 ISBN-13(EAN): 9781493985661
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.”

Автор: Kalyuzhny Alexander E.
Название: Handbook of Elispot: Methods and Protocols
ISBN: 149396111X ISBN-13(EAN): 9781493961115
Издательство: Springer
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Цена: 19740.00 р.
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Описание: Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics.

Автор: Janetzki
Название: Elispot for Rookies (and Experts Too)
ISBN: 3319452932 ISBN-13(EAN): 9783319452937
Издательство: Springer
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Цена: 13060.00 р.
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Описание: This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators.The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation.

Автор: Janetzki Sylvia
Название: Elispot for Rookies (and Experts Too)
ISBN: 3319832603 ISBN-13(EAN): 9783319832609
Издательство: Springer
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Цена: 9781.00 р.
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Описание:

Preface.- Overview.- Historic Overview.- Principles of Elispot.- Modifications.- Simplifications.- Coating Step.- Detection Step.- Adaptations.- Detection of specific antibodies.- Detection of multiple analytes in one well.- Dual color Elispot.- Fluorospot.- Summary.- Important Assay Details.- Reagents and Materials.- Elispot plates.- Antibody choices.- Enzymatic conjugates and substrates.- Fluorescent dyes.- Culture media.- Blocking buffers.- Washing buffers.- Important laboratory supplies.- Pipettes.- Filters.- Other supplies.- Automated Elispot reader.- Sample preparation.- Overview.- Whole blood and PBMC.- Blood draw and anticoagulants.- PBMC isolation.- Time frame between blood draw and PBMC isolation.- Immune cells obtained from tissue.- Freezing of cells.- Thawing of cells.- Cell counting and viability.- Overnight resting.- Isolation of sub-populations of cells.- Expansion of cells.- Planning the experiment.- Controls.- Negative controls.- Positive controls.- External controls.- Plate layout.- Timing.- The Elispot assay.- Coating.- Blocking.- Effector cell preparation.- Stimulant preparation.- Peptides and peptide pools.- Proteins and lysate.- Antigen presenters.- Control stimulants.- Reference samples.- Plating out cells and stimulants.- Incubation.- Removal of cells.- Spot development.- Enumeration of spots.- Data analysis.- Quality control.- Reporting guidelines.- Troubleshooting.- References.