Handbook of Elispot: Methods and Protocols, Kalyuzhny Alexander E.

Автор: Kalyuzhny, Alexander E
Название: Handbook of ELISPOT methods and protocols
ISBN: 1588294692 ISBN-13(EAN): 9781588294692
Издательство: Springer
Цена: 11872.00 р.
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Описание: In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

Автор: Kalyuzhny
Название: Handbook of ELISPOT
ISBN: 1493985663 ISBN-13(EAN): 9781493985661
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.”

Название: Handbook of elispot
ISBN: 1493993283 ISBN-13(EAN): 9781493993284
Издательство: Springer
Рейтинг:
Цена: 16769.00 р.
Наличие на складе: Поставка под заказ.

Описание: This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.”

Автор: Janetzki Sylvia
Название: Elispot for Rookies (and Experts Too)
ISBN: 3319832603 ISBN-13(EAN): 9783319832609
Издательство: Springer
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Цена: 9781.00 р.
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Описание:

Preface.- Overview.- Historic Overview.- Principles of Elispot.- Modifications.- Simplifications.- Coating Step.- Detection Step.- Adaptations.- Detection of specific antibodies.- Detection of multiple analytes in one well.- Dual color Elispot.- Fluorospot.- Summary.- Important Assay Details.- Reagents and Materials.- Elispot plates.- Antibody choices.- Enzymatic conjugates and substrates.- Fluorescent dyes.- Culture media.- Blocking buffers.- Washing buffers.- Important laboratory supplies.- Pipettes.- Filters.- Other supplies.- Automated Elispot reader.- Sample preparation.- Overview.- Whole blood and PBMC.- Blood draw and anticoagulants.- PBMC isolation.- Time frame between blood draw and PBMC isolation.- Immune cells obtained from tissue.- Freezing of cells.- Thawing of cells.- Cell counting and viability.- Overnight resting.- Isolation of sub-populations of cells.- Expansion of cells.- Planning the experiment.- Controls.- Negative controls.- Positive controls.- External controls.- Plate layout.- Timing.- The Elispot assay.- Coating.- Blocking.- Effector cell preparation.- Stimulant preparation.- Peptides and peptide pools.- Proteins and lysate.- Antigen presenters.- Control stimulants.- Reference samples.- Plating out cells and stimulants.- Incubation.- Removal of cells.- Spot development.- Enumeration of spots.- Data analysis.- Quality control.- Reporting guidelines.- Troubleshooting.- References.

Автор: Janetzki
Название: Elispot for Rookies (and Experts Too)
ISBN: 3319452932 ISBN-13(EAN): 9783319452937
Издательство: Springer
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Цена: 13060.00 р.
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Описание: This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators.The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation.

Автор: B. Paul Morgan
Название: Complement Methods and Protocols
ISBN: 1617371165 ISBN-13(EAN): 9781617371165
Издательство: Springer
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Цена: 21661.00 р.
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Описание: The complement system, first described more than a century ago, was for many years the ugly duckling of the immunology world, but no more.

Автор: Yan Qing
Название: Psychoneuroimmunology: Methods and Protocols
ISBN: 1493962604 ISBN-13(EAN): 9781493962600
Издательство: Springer
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Цена: 20263.00 р.
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Описание: As with its stable mates in the Methods in Molecular Biology series, this new book offers reproducible laboratory protocols and expert tips in the field of developing integrative and individualized therapeutics, with applications from drugs to behaviors.

Автор: Clausen Bjцrn E., Laman Jon D.
Название: Inflammation: Methods and Protocols
ISBN: 1493983008 ISBN-13(EAN): 9781493983001
Издательство: Springer
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Цена: 16070.00 р.
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Описание: Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated inflammatory disease models affecting barrier organs to the environment;

Автор: Albitar Maher
Название: Monoclonal antibodies / methods and protocols
ISBN: 1588295672 ISBN-13(EAN): 9781588295675
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins. Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.

Автор: Michael Steinitz (Editor)
Название: Human Monoclonal Antibodies: Methods and Protocols
ISBN: 1627035850 ISBN-13(EAN): 9781627035859
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This book details readily reproducible protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies. It also includes articles on related topics of human monoclonal and polyclonal antibodies.

Автор: Fox Christopher B.
Название: Vaccine Adjuvants: Methods and Protocols
ISBN: 1493982028 ISBN-13(EAN): 9781493982028
Издательство: Springer
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Цена: 16070.00 р.
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Описание: "Published In: United States, 08 October 2016" --Distributor`s website.

Автор: Almeida de Sousa
Название: DNA Vaccines: Methods and Protocols
ISBN: 1071608711 ISBN-13(EAN): 9781071608715
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.