Anandhakumar Chandran Advancing Development of Synthetic Gene Regulators 9789811348990

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Автор: Anandhakumar Chandran
Название: Advancing Development of Synthetic Gene Regulators
ISBN: 9789811348990
Издательство: Springer
Классификация:

ISBN-10: 9811348995
Обложка/Формат: Soft cover
Страницы: 114
Вес: 0.21 кг.
Дата издания: 2018
Серия: Springer Theses
Язык: English
Издание: Softcover reprint of
Иллюстрации: 44 illustrations, color; 5 illustrations, black and white; xv, 114 p. 49 illus., 44 illus. in color.
Размер: 234 x 156 x 7
Читательская аудитория: Professional & vocational
Основная тема: Chemistry
Подзаголовок: With the Power of High-Throughput Sequencing in Chemical Biology
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание:

This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.

Дополнительное описание: Overview of Next-Generation Sequencing Technologies and its application in Chemical Biology.- Next Generation Sequencing Studies Guide the Design of Pyrrole-Imidazole Polyamides with Improved Binding Specificity by the Addition of ?-alanine.- Genome-Wide

Advancing Development of Synthetic Gene Regulators, Anandhakumar Chandran