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Single Molecule Analysis, Erwin J. G. Peterman


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Автор: Erwin J. G. Peterman
Название:  Single Molecule Analysis
ISBN: 9781493984374
Издательство: Springer
Классификация:

ISBN-10: 1493984373
Обложка/Формат: Soft cover
Страницы: 369
Вес: 0.73 кг.
Дата издания: 2018
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 46 illustrations, color; 42 illustrations, black and white; xi, 369 p. 88 illus., 46 illus. in color.
Размер: Book (Paperback Initiative)
Читательская аудитория: Professional & vocational
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Single Molecule Analysis: Methods and Protocols, Second Edition aims to be useful to many researchers, inspire them and help them to go single molecule. 
Дополнительное описание: Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions.- RNA Unzipping and Force Measurements with a Dual Optical Trap.- Protein Tethering for Folding Studies.- Combining Structure-Function and Single-Molecule Studies on Cy



Single Molecule Analysis

Автор: Erwin J. G. Peterman
Название: Single Molecule Analysis
ISBN: 1493972707 ISBN-13(EAN): 9781493972708
Издательство: Springer
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Цена: 20962.00 р.
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Описание:

Part I

1. Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions
Joost van Mameren, Gijs J. L. Wuite, and Iddo Heller

2. RNA Unzipping and Force Measurements with a Dual Optical Trap
Laurent Geffroy, Pierre Mangeol, Thierry Bizebard, and Ulrich Bockelmann

3. Protein Tethering for Folding Studies
Fatemeh Moayed, Roeland J. van Wijk, David P. Minde, and Sander J. Tans

4. Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein
Lu Rao, Maren Hьlsemann, and Arne Gennerich

5. A Brief Introduction to Single-Molecule Fluorescence Methods
Siet M. J. L. van den Wildenberg, Bram Prevo, and Erwin J. G. Peterman

PART II

6. Fluorescent Labeling of Proteins
Mauro Modesti

7. Single-Molecule Imaging of Escherichia coli Trans-Membrane Proteins
Aravindan Varadarajan, Felix Oswald, and Yves J. M. Bollen

8. Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans
Jaap van Krugten and Erwin J. G. Peterman

9. Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy
Roderick P. Tas, Trusanne G. A. A. Bos, and Lukas C. Kapitein

10. Fluorescence Microscopy of Nanochannel-Confined DNA
Fredrik Westerlund, Fredrik Persson, Joachim Fritsche, Jason P. Beech, and Jonas O. Tegenfeldt

11. Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin--Microtubule Complexes
Matthieu P. M. H. Benoit and Hernando Sosa

12. Single Molecule FRET Analysis of DNA Binding Proteins
Kathy R. Chaurasiya and Remus T. Dame

PART III

13. Atomic Force Microscopy: An Introduction
Melissa C. Piontek and Wouter H. Roos

14. Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy
Malgorzata Grosbart, Dejan Ristic, Humberto Sбnchez, and Claire Wyman

15. Atomic Force Microscopy of Protein Shells: Virus Capsids and Beyond
Alvaro Ortega-Esteban, N. Martнn-Gonzбlez, F. Moreno-Madrid, Aida Llaurу, Mercedes Hernando-Pйrez, and Pedro J. de Pablo

16. Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single Molecule Manipulation and Visualization
Yeonee Seol and Keir C. Neuman

17. Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Transcription Factor Interactions
Daniel T. Tovari, Yan Yan, Laura Finzi, and David Dunlap

18. Single-Molecule Measurements Using Acoustic Force Spectroscopy (AFS)
Douwe Kamsma, and Gijs J.L. Wuite

19. Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy
Daren Yang, and Wesley P. Wong

Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein

Автор: Sharona Cohen
Название: Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein
ISBN: 331937236X ISBN-13(EAN): 9783319372365
Издательство: Springer
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Цена: 11753.00 р.
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Описание: Inthis thesis single-molecule fluorescence resonance energy transfer(FRET)spectroscopy was used to study the folding of a protein that belongsto the large and important family of repeat proteins.

Single molecule fluorescence spectroscopy of the folding of a repeat protein

Автор: Cohen, Sharona
Название: Single molecule fluorescence spectroscopy of the folding of a repeat protein
ISBN: 3319095579 ISBN-13(EAN): 9783319095578
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Inthis thesis single-molecule fluorescence resonance energy transfer(FRET)spectroscopy was used to study the folding of a protein that belongsto the large and important family of repeat proteins.

Single Molecule Biology,

Автор: Alexander E. Knight
Название: Single Molecule Biology,
ISBN: 0123742277 ISBN-13(EAN): 9780123742278
Издательство: Elsevier Science
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Цена: 11789.00 р.
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Описание: Describes such single molecule techniques (that allow for manipulation and measurement of single biological molecules within a live cell) as single molecule fluorescence, optical tweezers, and scanning probe microscopy. This book is organized by biological system or molecule. It discusses applications in molecular diagnostics and drug discovery.

Single molecule studies of nucleic acids and their associated proteins

Автор: Bensimon, David (research Director And Professor, Lps-ens, Psl, Cnrs And Department Of Chemistry And Biochemistry, Ucla, Usa) Croquette, Vincent (re
Название: Single molecule studies of nucleic acids and their associated proteins
ISBN: 0198530927 ISBN-13(EAN): 9780198530923
Издательство: Oxford Academ
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Цена: 11246.00 р.
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Описание: This book is an introduction to the novel physical tools that allow single molecule manipulation and visualisation. It describes the use of these tools to understand the elastic properties of single molecules of DNA and RNA and to study their interactions with the many proteins that process them.

Small Molecule Microarrays

Автор: Mahesh Uttamchandani; Shao Q. Yao
Название: Small Molecule Microarrays
ISBN: 1493965824 ISBN-13(EAN): 9781493965823
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a significant collection of examples, which serve to guide researchers toward ways in which SMM technology may be effectively deployed for multiplexed screening, drug discovery and ligand identification. The volume is organized into three sections covering microarray fabrication, imaging modes, and discovery approaches. The chapters cover a range of small molecule library types, ranging from synthetic combinatorial libraries to peptide and carbohydrate libraries, with lessons and techniques that can be widely applied. Written in the highly successful Methods in Molecular Biology series, format, chapters include introductions of their respective topics, lists of the requisite materials and reagents, step-by-step and readily reproducible laboratory protocols as well as vital tips on troubleshooting and advice on avoiding known pitfalls. Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous insights into the successful applications of SMM, by experts in the field. Building on this collection of knowledge, the volume seeks to inspire a future generation of microarray practitioners to propel this significant technology to even greater heights

Single Molecule Enzymology

Автор: Gregory I. Mashanov; Christopher Batters
Название: Single Molecule Enzymology
ISBN: 149396190X ISBN-13(EAN): 9781493961900
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This an ideal guide for the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail. Experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles.

Single-molecule Studies of Proteins

Автор: Andres F. Oberhauser
Название: Single-molecule Studies of Proteins
ISBN: 1489991123 ISBN-13(EAN): 9781489991126
Издательство: Springer
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Цена: 22201.00 р.
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Описание: This book examines the successful application of single-molecule techniques to a wide range of biological events. It explores sophisticated advances in powerful new analytical, computational, and imaging methods.


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