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Plant Epigenetics: Methods and Protocols, Kovalchuk Igor


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Автор: Kovalchuk Igor
Название:  Plant Epigenetics: Methods and Protocols
ISBN: 9781493979561
Издательство: Springer
Классификация:
ISBN-10: 1493979566
Обложка/Формат: Paperback
Страницы: 253
Вес: 0.46 кг.
Дата издания: 07.07.2018
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 28 tables, color; 32 illustrations, color; 19 illustrations, black and white; x, 253 p. 51 illus., 32 illus. in color.
Размер: 25.40 x 17.78 x 1.40 cm
Читательская аудитория: General (us: trade)
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание:

1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis

Wanhui You, Stйphane Pien, and Ueli Grossniklaus

2. Chromatin Conformation Capture-Based Analysis of Nuclear Architecture

Stefan Grob and Ueli Grossniklaus

3. Metaanalysis of Genome-Wide Chromatin Data

Julia Engelhorn and Franziska Turck

4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes

Sara Hernбndez-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peсa

5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns

Andriy Bilichak and Igor Kovalchuk

6. Analysis of Global Genome Methylation Using the Cytosine-Extension Assay

Andriy Bilichak and Igor Kovalchuk

7. In Situ Analysis Of DNA Methylation in Plants

Palak Kathiria and Igor Kovalchuk

8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay

Andrey Golubov and Igor Kovalchuk

9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)

Marнa Бngeles Guevara, Nuria De Marнa, Enrique Sбez-Laguna, Marнa Dolores Vйlez, Marнa Teresa Cervera, and Josй Antonio Cabezas

10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes

Pavlina Sasheva Ueli Grossniklaus

11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays

Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot

12. Northern Blotting Techniques for Small RNAs

Todd Blevins

13. Stem-Loop QRT-PCR for the Detection of Plant Micrornas

Erika Varkonyi-Gasic

14. Profiling New Small RNA Sequences

Masayuki Tsuzuki and Yuichiro Watanabe

15. Small RNA Library Preparation and Illumina Sequencing in Plants

Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk

16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow

Slava Ilnytskyy and Andriy Bilichak

17. Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing

Andriy Bilichak and Igor Kovalchuk

18. The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks

Andriy Bilichak and Igor Kovalchuk

19. Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species

Christian Parisod




Plant Epigenetics

Автор: Nikolaus Rajewsky; Stefan Jurga; Jan Barciszewski
Название: Plant Epigenetics
ISBN: 3319555197 ISBN-13(EAN): 9783319555195
Издательство: Springer
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Цена: 34937.00 р.
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Описание: Discussing areas ranging from the complex regulation of stress and heterosis to the precise mechanisms of DNA and histone modifications, it presents breakthroughs in our understanding of complex phenotypic phenomena.

Plant Epigenetics

Автор: Kovalchuk
Название: Plant Epigenetics
ISBN: 1489977066 ISBN-13(EAN): 9781489977069
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Thisvolume provides a variety of protocols to analyze various epigeneticchanges, including differential expression of non-coding RNAs, changes in DNAmethylation, and histone modifications in plants. Chapters detail protocolswith different degrees of complexity, and describe bioinformatics approachesfor data processing and analysis. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative andcutting-edge, Plant Epigenetics: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field.

Epigenetics in Plants of Agronomic Importance: Fundamentals and Applications

Автор: Ra?l Alvarez-Venegas; Clelia De-la-Pe?a; Juan Arma
Название: Epigenetics in Plants of Agronomic Importance: Fundamentals and Applications
ISBN: 3030147592 ISBN-13(EAN): 9783030147594
Издательство: Springer
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Цена: 22359.00 р.
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Описание: Over the past few decades, chromatin modulation has emerged as an important regulator of gene expression. This second edition provides detailed information on the epigenetic mechanisms in plants, illustrating the value of this research in plants of agronomic importance. It examines recent advances regarding plants’ epigenetic regulation in response to abiotic and biotic types of stress; the epigenetic basis of plant immunity; evolution and functions of plant histones; epigenetic variation and plant breeding; and epigenome editing and crop improvement. The content is intended to promote the development of future biotechnologies to manipulate and selectively activate/inhibit proteins and metabolic pathways to counter pathogens, to treat important diseases, and to increase crop productivity. The development of new fields, like epigenome editing and RNA epigenetics, will certainly improve our understanding of currently known epigenetic modifications and their roles in e.g. host-pathogen interactions, crop productivity, and in response to environmental stimuli. This volume contains twelve new/revised chapters, written by an international team of experts on plant epigenetics, and addresses the needs of researchers and professionals in the fields of agronomics, crop breeding, epigenetics, plant biochemistry, plant developmental biology, and related disciplines.

Plant Epigenetics and Epigenomics: Methods and Protocols

Автор: Spillane Charles, McKeown Peter
Название: Plant Epigenetics and Epigenomics: Methods and Protocols
ISBN: 1071601784 ISBN-13(EAN): 9781071601785
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.

Plant Epigenetics and Epigenomics

Автор: Charles Spillane; Peter C. McKeown
Название: Plant Epigenetics and Epigenomics
ISBN: 1493962299 ISBN-13(EAN): 9781493962297
Издательство: Springer
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Цена: 14673.00 р.
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Описание: Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants.

Plant Epigenetics

Автор: Igor Kovalchuk; Franz J. Zemp
Название: Plant Epigenetics
ISBN: 1607616459 ISBN-13(EAN): 9781607616450
Издательство: Springer
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Цена: 20263.00 р.
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Описание: Epigenetics is one of the most popular and quickly evolving fields of modern science. In this book, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants.

Plant Epigenetics

Автор: Igor Kovalchuk; Franz J. Zemp
Название: Plant Epigenetics
ISBN: 1493957562 ISBN-13(EAN): 9781493957569
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
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Описание: The discovery of DNA as the genetic material brought great hope to scientists all over the world. It was believed that many of the lingering questions in genetics and the mechanisms of heredity would fnally be answered. However, as often is the case in science, more qu- tions arose out of this discovery. What defnes a gene? What are the mechanisms of gene regulation? Further discovery and technological innovations brought about sequencing techniques that allowed the study of complete genomes from many organisms, including Arabidopsis and humans. Despite all the excitement surrounding these technologies, many features of the genome remained unclear. Peculiar characteristics in genome composition such as signifcant redundancy consisting of many repetitive elements and noncoding sequences, active transcriptional units with no protein product, and unusual sequences in promoter regions added to the mysteries of genetic make-up and gene regulation. Indeed, the more we discovered about the genome, the more diffcult it became to understand the complexity of cellular function and regulation. Out of the study of the intricacies of the genome and gene regulation, arose a new science that was independent of actual DNA changes, but critical in maintaining gene regulation and genetic stability. Epigenetics, literally translated as “above genetics,” is the science that describes the mechanisms of heritable changes in gene regulation that does not involve modifcations of DNA sequence. These changes may last through somatic cell division and, in some cases, throughout multiple generations.

Plant Epigenetics and Epigenomics

Автор: Charles Spillane; Peter C. McKeown
Название: Plant Epigenetics and Epigenomics
ISBN: 1627037721 ISBN-13(EAN): 9781627037723
Издательство: Springer
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Цена: 20962.00 р.
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Описание: Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants.

Plant Epigenetics

Автор: Rajewsky Nikolaus, Jurga Stefan, Barciszewski Jan
Название: Plant Epigenetics
ISBN: 3319856847 ISBN-13(EAN): 9783319856841
Издательство: Springer
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Цена: 27950.00 р.
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Описание: This book presents, in 26 chapters, the status quo in epigenomic profiling. It discusses how functional information can be indirectly inferred and describes the new approaches that promise functional answers, collectively referred to as epigenome editing. It highlights the latest important advances in our understanding of the functions of plant epigenomics and new technologies for the study of epigenomic marks and mechanisms in plants. Topics include the deposition or removal of chromatin modifications and histone variants, the role of epigenetics in development and response to environmental signals, natural variation and ecology, as well as applications for epigenetics in crop improvement. Discussing areas ranging from the complex regulation of stress and heterosis to the precise mechanisms of DNA and histone modifications, it presents breakthroughs in our understanding of complex phenotypic phenomena.

Epigenetics and Breeding

Автор: Gallusci, Philippe
Название: Epigenetics and Breeding
ISBN: 0128154039 ISBN-13(EAN): 9780128154038
Издательство: Elsevier Science
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Цена: 24928.00 р.
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Описание:

Epigenetics and Breeding, Volume 88, the latest release in the Advances in Botanical Research series, brings together the experiences and critical information teachers, researchers and managers must consider from both scientific and legal points-of-view as they relate to biotechnology. New chapters in this updated volume include sections on Epigenetic Mechanisms in Plants, Epigenomic Diversity and Applications to Breeding, Epigenetics in Breeding, EpiRILs: Lessons from Arabidopsis, Transposable Elements as a Tool for Plant Improvement, Epigenome Editing, Epigenetics and Grafting, Sexual and Non-sexual reproduction, Epigenetics in Cereals, and more.

  • Encompasses various aspects of botanical research, including its historical background, current status, recent research outcomes and potential future developments
  • Written by highly competent authors from all continents
  • Provides data that is based on facts and written in a dispassionate and non-polemical tone
Epigenetics in Plants of Agronomic Importance: Fundamentals and Applications

Автор: Ra?l Alvarez-Venegas; Clelia De la Pe?a; Juan Arma
Название: Epigenetics in Plants of Agronomic Importance: Fundamentals and Applications
ISBN: 3319358154 ISBN-13(EAN): 9783319358154
Издательство: Springer
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Цена: 16977.00 р.
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Описание: Furthermore, we provide important insight on how the plant epigenome changes in response to developmental or environmental stimuli, how chromatin modifications are established and maintained, to which degree they are used throughout the genome, and how chromatin modifications influence each another.

Plant Gene Regulatory Networks: Methods and Protocols

Автор: Kaufmann Kerstin, Mueller-Roeber Bernd
Название: Plant Gene Regulatory Networks: Methods and Protocols
ISBN: 149398392X ISBN-13(EAN): 9781493983926
Издательство: Springer
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Цена: 22359.00 р.
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Описание:

Preface...
Table of Contents...

Contributing Authors...

1. From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants

Kerstin Kaufmann and Dijun Chen

Part I Experimental Approaches to Study Plant Gene-Regulatory Networks


2. Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis
Bennett Thomson, Emmanuelle Graciet, and Frank Wellmer


3. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting
Daniel Slane and Martin Bayer


4. Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation
On Sun Lau


5. Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries
Rocнo Sбnchez-Montesino and Luis Oсate-Sбnchez


6.
SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors
Cezary Smaczniak, Gerco C. Angenent and Kerstin Kaufmann


7. Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems
Sara Dнaz-Triviсo, Yuchen Long, Ben Scheres, and Ikram Blilou


8.
Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors
Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, and Klaus D. Grasser

9. Characterization of Mediator Complex and its Associated Proteins from Rice
Subhasis Samanta and Jitendra Kumar Thakur


10.
DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing
Sergei A. Filichkin and Molly Megraw


11. In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale
Chang Liu


12. Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems
Levi G. Lowder, Joseph W. Paul III, and Yiping Qi


13. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants
Tom Schreiber and Alain Tissier

Part II Computational Approaches to Study Plant Gene-Regulatory Networks


14.
Design of Knowledge Bases for Plant Gene Regulatory Networks
Eric Mukundi, Fabio Gomez-Cano, Wilberforce Zachary Ouma, and Erich Grotewold


15. AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species
Tak Lee and Insuk Lee


16. Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development
Dijun Chen and Kerstin Kaufmann


17. Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data
Chun-Ping Yu and Wen-Hsiung Li


18. Computational Approaches to Study Gene Regulatory Networks
Nooshin Omranian and Zoran Nikoloski

19. Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction
J. Davila-Velderrain, J.C. Martinez-Garcia, and R.E. Alvarez Buylla


20. ODE-Based Modeling of Complex Regulatory Circuits
Daniel D. Seaton

21. Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach
Maria Ang


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