Описание: This Volume presents applications of hydrocarbon microbiology in the context of environmental pollutant degradation, covering pollutants such as petroleum and related wastes (i.e.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366249129X ISBN-13(EAN): 9783662491294 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction: Single cell biotechnology for uncultured microorganisms.- Single-cell cultivation in microfluidic devices.- Single-cell bacterial bioreporter assays to measure hydrocarbons.- Whole genome amplification of single bacterial cells.- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria.- Localisation of proteins within intact bacterial cells using fluorescent protein fusions.- Raman-FISH.- Single-cell metabolomics.- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division.- Single cell biotechnology for uncultured microorganisms.- Single cell and systems biology tools for biofuel production.- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT).- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity.- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence.
Автор: McGenity Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504332 ISBN-13(EAN): 9783662504338 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume presents a comprehensive series of generic protocols for the genetic and genomic analysis of prokaryotic isolates. Genetic methods for functional analyses employ the latest cloning vectors, gene fusion methods and transposon mutagenesis systems, as well as systems for introducing protease-cleavage sequences into permissive sites in proteins under investigation. Genomic methods described include protocols for transcriptomics, shotgun proteomics, interactomics, metabolic profiling, and lipidomics. Bioinformatic tools for genome annotation, transcriptome display and the integration of transcriptomic data into genome-scale metabolic reconstructions are described. Protocols for 13C-based metabolic flux determinations and analysis of the hierarchical and metabolic regulation of fluxes through pathways are included. The Volume thus enables investigators to functionally analyse an isolate over the entire cellular range spanning the gene, the genome, the transcript repertoire, the proteome, the interactome, the metabolic network with its nodes and their regulatory hierarchies, and the metabolic fluxes and their physiological controls.Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662527766 ISBN-13(EAN): 9783662527764 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Название: Hydrocarbon and lipid microbiology protocols ISBN: 3662451786 ISBN-13(EAN): 9783662451786 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction to the cultivation of microbes involved in the cycling of hydrocarbons.- Cultivating fastidious microbes.- The essentials of cultivating aerobic hydrocarbon-degrading microbes.- Cultivating aerobic hydrocarbon-degrading microbes from microalgae.- Growing and maintaining hydrocarbon-degrading consortia.- Two-phase cultivation techniques for hydrocarbon-degrading organisms.- Cultivation of hydrocarbon-degrading fungi.- Growth of hydrocarbon-degrading bacteria in continuous culture.- Growth procedures at low substrate concentrations.- Cultivation of biofilm-forming hydrocarbon degraders.- Cultivation of asphaltene-degrading microbes.- Cultivation of microorganisms for fuel quality improvement.- Simulation of oxygen and redox oscillations in crude oil- degrading bioreactors.- Cultivation of anaerobic microorganisms with hydrocarbons as growth substrates.- Enrichment and isolation of metal respirers and hydrocarbon oxidizers.- Enrichment and isolation of oxyion-respiring hydrocarbon oxidizers.- Cultivation of methanogens.- Cultivation of methylotrophs.- Cultivation of methanotrophs.- Cultivation of extremophilic hydrocarbon degraders.- Cultivation of piezophilic hydrocarbon degraders.- Cultivation of oil-producing microalgae.- Cultivation of oleaginous yeasts.- Cultivation of oil-tolerant protists.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366253116X ISBN-13(EAN): 9783662531167 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume describes methods for investigating microbes in their natural environment and how to obtain representative samples and preserve them for subsequent analyses. Chapters are arranged according to the environments under investigation, which include: oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and their tailing ponds, lakes, rivers, leaves, polar seas and ice, the sea-surface microlayer, mud flats, microbialites, and deep-sea fauna. A variety of downstream analytical procedures are described, including: nucleic-acid extraction and preparation for high-throughput sequencing, fluorescence in-situ hybridisation, and cultivation of aerobic and anaerobic hydrocarbon-degrading microbes. Though most chapters focus on hydrocarbon-rich environments, many of the approaches used are generic, and as such will be of value to researchers embarking on studies of microbes and their processes in the field.Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Описание: This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities.
Автор: McGenity Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504308 ISBN-13(EAN): 9783662504307 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise “reducing power availability”, and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. The Volume also includes an overview of how synthetic biology approaches can be used to improve biocontainment.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366249132X ISBN-13(EAN): 9783662491324 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells.
Описание: Introduction to computer-assisted analysis in lipid and hydrocarbon microbiology.- Application of Ecological Network Theory.- Statistical tools for data analysis.- Statistical tools for study design and replication.- MG-RAST, a metagenomics service for analysis of microbial community structure and function.- Using QIIME to evaluate the microbial communities within hydrocarbon environments.- Biodegradation Prediction Tools.- Predicting protein interactions.- Syntax and Semantics of Coding in Python.- Protocols for calculating reaction kinetics and thermodynamics.- Modelling the environmental fate of hydrocarbons during bioremediation.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662531135 ISBN-13(EAN): 9783662531136 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Описание: Introduction to Microbial Quantitation, Community Profiling, and Array Approaches.- A cell extraction method for oily sediments.- Introduction to microplate MPN-enumeration of hydrocarbon degraders.- Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs.- Microbial biomass and community analysis by PLFA analysis.- Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities.- Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities.- Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis.- Microbial community profiling: SSCP and T-RFLP techniques.- Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) - preparation, data handling and ecological analysis.- Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment.- Studying protistan communities in hydrocarbon-contaminated environments.
ООО "Логосфера " Тел:+7(495) 980-12-10 www.logobook.ru
