Hydrocarbon and Lipid Microbiology Protocols: Synthetic and Systems Biology - Applications, McGenity Terry J., Timmis Kenneth N., Nogales Fernбndez Balbina
Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina Название: Hydrocarbon and Lipid Microbiology Protocols: Activities and Phenotypes ISBN: 366256985X ISBN-13(EAN): 9783662569856 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction.- Protocols for the measurement of bacterial chemotaxis to hydrocarbons.- Determining the tendency of microorganisms to interact with hydrocarbon phases.- Protocols for measuring biosurfactant production in microbial cultures.- Protocol for the measurement of hydrocarbon transport in bacteria.- Bacterial solvent response and tolerance: cis-trans isomerization.- Analysis of PHB metabolism applying Tn5 mutagenesis in Ralstonia eutropha.- Phenotyping microarrays for the characterization of environmental microorganisms.- Control of the concentration of aquatic hydrocarbons by microorganisms.- Protocols for measuring methanogenesis.- Respiration rate determined by phosphorescence-based sensors.- Measuring the impact of hydrocarbons on rates of nitrogen fixation.- Protocols for measuring activity of sulphate-reducing bacteria in water injection systems.- Protocol for evaluating the biological stability of fuel formulations and their relationship to carbon steel biocorrosion.- Laboratory protocols for investigating microbial souring and potential treatments in crude oil reservoirs.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662491389 ISBN-13(EAN): 9783662491386 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes’ more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described.<
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: McGenity Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504332 ISBN-13(EAN): 9783662504338 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume presents a comprehensive series of generic protocols for the genetic and genomic analysis of prokaryotic isolates. Genetic methods for functional analyses employ the latest cloning vectors, gene fusion methods and transposon mutagenesis systems, as well as systems for introducing protease-cleavage sequences into permissive sites in proteins under investigation. Genomic methods described include protocols for transcriptomics, shotgun proteomics, interactomics, metabolic profiling, and lipidomics. Bioinformatic tools for genome annotation, transcriptome display and the integration of transcriptomic data into genome-scale metabolic reconstructions are described. Protocols for 13C-based metabolic flux determinations and analysis of the hierarchical and metabolic regulation of fluxes through pathways are included. The Volume thus enables investigators to functionally analyse an isolate over the entire cellular range spanning the gene, the genome, the transcript repertoire, the proteome, the interactome, the metabolic network with its nodes and their regulatory hierarchies, and the metabolic fluxes and their physiological controls.Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: McGenity Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504278 ISBN-13(EAN): 9783662504277 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662531135 ISBN-13(EAN): 9783662531136 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Описание: This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology.
Описание: This Volume presents applications of hydrocarbon microbiology in the context of environmental pollutant degradation, covering pollutants such as petroleum and related wastes (i.e.
Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina Название: Hydrocarbon and Lipid Microbiology Protocols: Isolation and Cultivation ISBN: 3662568780 ISBN-13(EAN): 9783662568781 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction to the cultivation of microbes involved in the cycling of hydrocarbons.- Cultivating fastidious microbes.- The essentials of cultivating aerobic hydrocarbon-degrading microbes.- Cultivating aerobic hydrocarbon-degrading microbes from microalgae.- Growing and maintaining hydrocarbon-degrading consortia.- Two-phase cultivation techniques for hydrocarbon-degrading organisms.- Cultivation of hydrocarbon-degrading fungi.- Growth of hydrocarbon-degrading bacteria in continuous culture.- Growth procedures at low substrate concentrations.- Cultivation of biofilm-forming hydrocarbon degraders.- Cultivation of asphaltene-degrading microbes.- Cultivation of microorganisms for fuel quality improvement.- Simulation of oxygen and redox oscillations in crude oil- degrading bioreactors.- Cultivation of anaerobic microorganisms with hydrocarbons as growth substrates.- Enrichment and isolation of metal respirers and hydrocarbon oxidizers.- Enrichment and isolation of oxyion-respiring hydrocarbon oxidizers.- Cultivation of methanogens.- Cultivation of methylotrophs.- Cultivation of methanotrophs.- Cultivation of extremophilic hydrocarbon degraders.- Cultivation of piezophilic hydrocarbon degraders.- Cultivation of oil-producing microalgae.- Cultivation of oleaginous yeasts.- Cultivation of oil-tolerant protists.
Описание: Introduction: Single cell biotechnology for uncultured microorganisms.- Single-cell cultivation in microfluidic devices.- Single-cell bacterial bioreporter assays to measure hydrocarbons.- Whole genome amplification of single bacterial cells.- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria.- Localisation of proteins within intact bacterial cells using fluorescent protein fusions.- Raman-FISH.- Single-cell metabolomics.- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division.- Single cell biotechnology for uncultured microorganisms.- Single cell and systems biology tools for biofuel production.- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT).- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity.- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 3662504499 ISBN-13(EAN): 9783662504499 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities.
Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga Название: Hydrocarbon and Lipid Microbiology Protocols ISBN: 366252791X ISBN-13(EAN): 9783662527917 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function.
Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina Название: Hydrocarbon and Lipid Microbiology Protocols: Meso- And Microcosms ISBN: 3662571129 ISBN-13(EAN): 9783662571125 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: < Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function.
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